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Abstract:

Cruzipain, purified by conventional methods, and Ag163B6, isolated by affinity chromatography with a monoclonal antibody raised against a T. cruzi extract, are glycoproteins with a similar electrophoretic mobility, which reacted with sera from most chronic chagasic patients. Their behaviour in SDS-PAGE, Western blotting, isoelectric focusing, two-dimensional electrophoresis (IEF and SDS-PAGE), Ouchterlony's double diffusion, and enzyme activity in SDS-PAGE gels containing 0.1% gelatin suggests that they are identical. © 1993.

Registro:

Documento: Artículo
Título:Identity of the major cysteine proteinase (cruzipain) from Trypanosoma cruzi and an antigen (Ag163B6) isolated with a monoclonal antibody
Autor:Malchiodi, E.L.; Chiaramonte, M.G.; Martinez, J.A.; Zwirner, N.W.; Margni, R.A.; Cazzulo, J.J.
Filiación:Instituto de Estudios de la Immunidad Humoral (CONICET/UBA), Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina
Instituto de Investigaciones Bioquímicas Luis F. Leloir, Fundación Campomar (CONICET), Facultad de Ciencias Exactas y Naturales, Buenos Aires, Argentina
Palabras clave:Ag163B6; Cruzipain; monoclonal antibody; parasite antigen; affinity chromatography; article; mouse; nonhuman; priority journal; trypanosoma cruzi; Animal; Antibodies, Monoclonal; Antigens, Protozoan; Blotting, Western; Cysteine Endopeptidases; Electrophoresis, Gel, Two-Dimensional; Immunodiffusion; Isoelectric Focusing; Support, Non-U.S. Gov't; Trypanosoma cruzi
Año:1993
Volumen:35
Número:1
Página de inicio:59
Página de fin:62
DOI: http://dx.doi.org/10.1016/0165-2478(93)90148-U
Título revista:Immunology Letters
Título revista abreviado:Immunol. Lett.
ISSN:01652478
CODEN:IMLED
CAS:Antibodies, Monoclonal; Antigens, Protozoan; cruzipain, EC 3.4.22.-; Cysteine Endopeptidases, EC 3.4.22
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01652478_v35_n1_p59_Malchiodi

Referencias:

  • Cazzulo, (1991) Biochemical Protozoology, pp. 191-199. , G.H. Coombs, M.J. North, Taylor & Francis, London
  • Martinez, Campetella, Frasch, Cazzulo, (1991) Infect. Immun., 59, p. 4275
  • Bontempi, Cazzulo, (1990) FEMS Microbiol. Lett., 70, p. 337
  • Souto-Padrón, Campetella, Cazzulo, de Souza, (1990) J. Cell Sci., 96, p. 485
  • Martínez, Cazzulo, (1992) FEMS Microbiol. Lett., 95, p. 225
  • Carbonetto, Malchiodi, Chiaramonte, Durante de isola, Fossati, Margni, (1990) Clin. Exp. Immunol., 82, p. 93
  • Cazzulo, Couso, Raimondi, Wernstedt, Hellman, (1989) Mol. Biochem. Parasitol., 33, p. 33
  • Campetella, Martínez, Cazzulo, (1990) FEMS Microbiol. Lett., 67, p. 145
  • Ouchterlony, (1958) Prog. Allergy, 5, p. 1
  • Laemmli, (1970) Nature, 227, p. 680
  • Merril, Goldman, Sedman, Ebert, (1981) Science, 211, p. 1437
  • Towbin, Staehelin, Gordon, (1979) Proc. Natl. Acad. Sci. USA, 76, p. 4350
  • Righetti, (1983) Isoelectric focusing: theory, methodology and applications, , T.S. Work, R.H. Burdon, Elsevier Science Publishers, Amsterdam
  • Stott, (1989) J. Immunol. Methods, 119, p. 153
  • O'Farrell, Goodman, O'Farrell, (1977) Cell, 12, p. 1133
  • Campetella, Henriksson, Åslund, Frasch, Pettersson, Cazzulo, (1992) Mol. Biochem. Parasitol., 50, p. 225
  • Scharfstein, Schechter, Senna, Peralta, Mendonça-Previato, Miles, (1988) J. Immunol., 137, p. 1336
  • Murta, Persechini, de Souto Padrón, de Souza, Guimaraes, Scharfstein, (1990) Mol. Biochem. Parasitol., 43, p. 27

Citas:

---------- APA ----------
Malchiodi, E.L., Chiaramonte, M.G., Martinez, J.A., Zwirner, N.W., Margni, R.A. & Cazzulo, J.J. (1993) . Identity of the major cysteine proteinase (cruzipain) from Trypanosoma cruzi and an antigen (Ag163B6) isolated with a monoclonal antibody. Immunology Letters, 35(1), 59-62.
http://dx.doi.org/10.1016/0165-2478(93)90148-U
---------- CHICAGO ----------
Malchiodi, E.L., Chiaramonte, M.G., Martinez, J.A., Zwirner, N.W., Margni, R.A., Cazzulo, J.J. "Identity of the major cysteine proteinase (cruzipain) from Trypanosoma cruzi and an antigen (Ag163B6) isolated with a monoclonal antibody" . Immunology Letters 35, no. 1 (1993) : 59-62.
http://dx.doi.org/10.1016/0165-2478(93)90148-U
---------- MLA ----------
Malchiodi, E.L., Chiaramonte, M.G., Martinez, J.A., Zwirner, N.W., Margni, R.A., Cazzulo, J.J. "Identity of the major cysteine proteinase (cruzipain) from Trypanosoma cruzi and an antigen (Ag163B6) isolated with a monoclonal antibody" . Immunology Letters, vol. 35, no. 1, 1993, pp. 59-62.
http://dx.doi.org/10.1016/0165-2478(93)90148-U
---------- VANCOUVER ----------
Malchiodi, E.L., Chiaramonte, M.G., Martinez, J.A., Zwirner, N.W., Margni, R.A., Cazzulo, J.J. Identity of the major cysteine proteinase (cruzipain) from Trypanosoma cruzi and an antigen (Ag163B6) isolated with a monoclonal antibody. Immunol. Lett. 1993;35(1):59-62.
http://dx.doi.org/10.1016/0165-2478(93)90148-U