Abstract:
Cruzipain, purified by conventional methods, and Ag163B6, isolated by affinity chromatography with a monoclonal antibody raised against a T. cruzi extract, are glycoproteins with a similar electrophoretic mobility, which reacted with sera from most chronic chagasic patients. Their behaviour in SDS-PAGE, Western blotting, isoelectric focusing, two-dimensional electrophoresis (IEF and SDS-PAGE), Ouchterlony's double diffusion, and enzyme activity in SDS-PAGE gels containing 0.1% gelatin suggests that they are identical. © 1993.
Registro:
Documento: |
Artículo
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Título: | Identity of the major cysteine proteinase (cruzipain) from Trypanosoma cruzi and an antigen (Ag163B6) isolated with a monoclonal antibody |
Autor: | Malchiodi, E.L.; Chiaramonte, M.G.; Martinez, J.A.; Zwirner, N.W.; Margni, R.A.; Cazzulo, J.J. |
Filiación: | Instituto de Estudios de la Immunidad Humoral (CONICET/UBA), Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina Instituto de Investigaciones Bioquímicas Luis F. Leloir, Fundación Campomar (CONICET), Facultad de Ciencias Exactas y Naturales, Buenos Aires, Argentina
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Palabras clave: | Ag163B6; Cruzipain; monoclonal antibody; parasite antigen; affinity chromatography; article; mouse; nonhuman; priority journal; trypanosoma cruzi; Animal; Antibodies, Monoclonal; Antigens, Protozoan; Blotting, Western; Cysteine Endopeptidases; Electrophoresis, Gel, Two-Dimensional; Immunodiffusion; Isoelectric Focusing; Support, Non-U.S. Gov't; Trypanosoma cruzi |
Año: | 1993
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Volumen: | 35
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Número: | 1
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Página de inicio: | 59
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Página de fin: | 62
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DOI: |
http://dx.doi.org/10.1016/0165-2478(93)90148-U |
Título revista: | Immunology Letters
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Título revista abreviado: | Immunol. Lett.
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ISSN: | 01652478
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CODEN: | IMLED
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CAS: | Antibodies, Monoclonal; Antigens, Protozoan; cruzipain, EC 3.4.22.-; Cysteine Endopeptidases, EC 3.4.22
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01652478_v35_n1_p59_Malchiodi |
Referencias:
- Cazzulo, (1991) Biochemical Protozoology, pp. 191-199. , G.H. Coombs, M.J. North, Taylor & Francis, London
- Martinez, Campetella, Frasch, Cazzulo, (1991) Infect. Immun., 59, p. 4275
- Bontempi, Cazzulo, (1990) FEMS Microbiol. Lett., 70, p. 337
- Souto-Padrón, Campetella, Cazzulo, de Souza, (1990) J. Cell Sci., 96, p. 485
- Martínez, Cazzulo, (1992) FEMS Microbiol. Lett., 95, p. 225
- Carbonetto, Malchiodi, Chiaramonte, Durante de isola, Fossati, Margni, (1990) Clin. Exp. Immunol., 82, p. 93
- Cazzulo, Couso, Raimondi, Wernstedt, Hellman, (1989) Mol. Biochem. Parasitol., 33, p. 33
- Campetella, Martínez, Cazzulo, (1990) FEMS Microbiol. Lett., 67, p. 145
- Ouchterlony, (1958) Prog. Allergy, 5, p. 1
- Laemmli, (1970) Nature, 227, p. 680
- Merril, Goldman, Sedman, Ebert, (1981) Science, 211, p. 1437
- Towbin, Staehelin, Gordon, (1979) Proc. Natl. Acad. Sci. USA, 76, p. 4350
- Righetti, (1983) Isoelectric focusing: theory, methodology and applications, , T.S. Work, R.H. Burdon, Elsevier Science Publishers, Amsterdam
- Stott, (1989) J. Immunol. Methods, 119, p. 153
- O'Farrell, Goodman, O'Farrell, (1977) Cell, 12, p. 1133
- Campetella, Henriksson, Åslund, Frasch, Pettersson, Cazzulo, (1992) Mol. Biochem. Parasitol., 50, p. 225
- Scharfstein, Schechter, Senna, Peralta, Mendonça-Previato, Miles, (1988) J. Immunol., 137, p. 1336
- Murta, Persechini, de Souto Padrón, de Souza, Guimaraes, Scharfstein, (1990) Mol. Biochem. Parasitol., 43, p. 27
Citas:
---------- APA ----------
Malchiodi, E.L., Chiaramonte, M.G., Martinez, J.A., Zwirner, N.W., Margni, R.A. & Cazzulo, J.J.
(1993)
. Identity of the major cysteine proteinase (cruzipain) from Trypanosoma cruzi and an antigen (Ag163B6) isolated with a monoclonal antibody. Immunology Letters, 35(1), 59-62.
http://dx.doi.org/10.1016/0165-2478(93)90148-U---------- CHICAGO ----------
Malchiodi, E.L., Chiaramonte, M.G., Martinez, J.A., Zwirner, N.W., Margni, R.A., Cazzulo, J.J.
"Identity of the major cysteine proteinase (cruzipain) from Trypanosoma cruzi and an antigen (Ag163B6) isolated with a monoclonal antibody"
. Immunology Letters 35, no. 1
(1993) : 59-62.
http://dx.doi.org/10.1016/0165-2478(93)90148-U---------- MLA ----------
Malchiodi, E.L., Chiaramonte, M.G., Martinez, J.A., Zwirner, N.W., Margni, R.A., Cazzulo, J.J.
"Identity of the major cysteine proteinase (cruzipain) from Trypanosoma cruzi and an antigen (Ag163B6) isolated with a monoclonal antibody"
. Immunology Letters, vol. 35, no. 1, 1993, pp. 59-62.
http://dx.doi.org/10.1016/0165-2478(93)90148-U---------- VANCOUVER ----------
Malchiodi, E.L., Chiaramonte, M.G., Martinez, J.A., Zwirner, N.W., Margni, R.A., Cazzulo, J.J. Identity of the major cysteine proteinase (cruzipain) from Trypanosoma cruzi and an antigen (Ag163B6) isolated with a monoclonal antibody. Immunol. Lett. 1993;35(1):59-62.
http://dx.doi.org/10.1016/0165-2478(93)90148-U