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Abstract:

High affinity binding of [3H]dihydroergocryptine (DHE) to crude membrane preparations of bovine and rat pineal glands was examined by a rapid filtration procedure through Whatman GFB paper. Scatchard analysis of bovine pineal membranes revealed a single population of binding sites with Kd = (4.8 ± 1.9) × 10−8M (mean±S.E.M., n= 3 and binding site concentration= 834 ± 103 fmol/mg of protein. When various α- and β-adrenergic agonists or antagonists were tested for their ability to inhibit [3H]DHE binding the following Ki values (μM) were obtained: DHE (0.039), phentolamine (0.102), phenoxybenzomine (0.262), methysergide (0.670), l-epinephrine (2.27), serotonin (3.09), l-norepinephrine (3.20), octopamine (30.3), l-propranolol (1710), l-isoproterenol (〉2000). In order to obtain information on the pre- or postsynaptic location of pineal DHE binding sites, pineal 900 g supernatants were prepared from rats subjected to bilateral superior cervical ganglionectomy or sham-operation 7 days earlier. Ganglionectomy caused a 53% increase in the number of binding sites without changing significantly their Kd (5.2 × 10−8 M and 5.5 × 10−8 M in ganglionectomized and control rats, respectively). These results are compatible with the view that postsynaptic α-adrenoceptors are present in the pineal gland. © 1980, Elsevier/North-Holland Biomedical Press. All rights reserved.

Registro:

Documento: Artículo
Título:Dihydroergocryptine binding sites in bovine and rat pineal glands
Autor:Vacas, M.I.; Lowenstein, P.R.; Cardinali, D.P.
Filiación:Centro de Estudios Farmacolo´gicos y de Principios Naturales (CEFAPRIN), V. de Obligado 2490, Buenos Aires, 1428, Argentina
Palabras clave:alpha-adrenoceptors; pineal gland; superior cervical ganglion; [3H]dihydroergocryptine binding; adrenalin; alpha adrenergic receptor; dihydroergocryptine h 3; drug receptor; isoprenaline; methysergide; noradrenalin; octopamine; oxidopamine; phenoxybenzamine; phentolamine; postsynaptic receptor; propranolol; radioisotope; serotonin; unclassified drug; animal experiment; article; autonomic nervous system; cattle; endocrine system; in vitro study; pharmacokinetics; pineal body; rat; Scatchard plot; superior cervical ganglion
Año:1980
Volumen:2
Número:4
Página de inicio:305
Página de fin:313
DOI: http://dx.doi.org/10.1016/0165-1838(80)90029-6
Título revista:Journal of the Autonomic Nervous System
Título revista abreviado:J. Auton. Nerv. Syst.
ISSN:01651838
CAS:isoprenaline, 299-95-6, 51-30-9, 6700-39-6, 7683-59-2; methysergide, 16509-15-2, 361-37-5, 62288-72-6; noradrenalin, 1407-84-7, 51-41-2; octopamine, 104-14-3; oxidopamine, 1199-18-4, 28094-15-7, 636-00-0; phenoxybenzamine, 59-96-1, 63-92-3; phentolamine, 50-60-2, 73-05-2; propranolol, 13013-17-7, 318-98-9, 3506-09-0, 4199-09-1, 525-66-6; serotonin, 50-67-9
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01651838_v2_n4_p305_Vacas

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Citas:

---------- APA ----------
Vacas, M.I., Lowenstein, P.R. & Cardinali, D.P. (1980) . Dihydroergocryptine binding sites in bovine and rat pineal glands. Journal of the Autonomic Nervous System, 2(4), 305-313.
http://dx.doi.org/10.1016/0165-1838(80)90029-6
---------- CHICAGO ----------
Vacas, M.I., Lowenstein, P.R., Cardinali, D.P. "Dihydroergocryptine binding sites in bovine and rat pineal glands" . Journal of the Autonomic Nervous System 2, no. 4 (1980) : 305-313.
http://dx.doi.org/10.1016/0165-1838(80)90029-6
---------- MLA ----------
Vacas, M.I., Lowenstein, P.R., Cardinali, D.P. "Dihydroergocryptine binding sites in bovine and rat pineal glands" . Journal of the Autonomic Nervous System, vol. 2, no. 4, 1980, pp. 305-313.
http://dx.doi.org/10.1016/0165-1838(80)90029-6
---------- VANCOUVER ----------
Vacas, M.I., Lowenstein, P.R., Cardinali, D.P. Dihydroergocryptine binding sites in bovine and rat pineal glands. J. Auton. Nerv. Syst. 1980;2(4):305-313.
http://dx.doi.org/10.1016/0165-1838(80)90029-6