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Abstract:

Previous studies in our laboratory indicated that immunization of male and female Wistar and Lewis rats with epididymal protein DE, resulted in the development of anti-DE antibodies in over 90% of the animals, with a significant and reversible reduction of fertility. In the present study, ELISA assays performed to analyze the evolution of the immune response indicated that antibody levels in the sera of immunized animals reached a maximum at 8 weeks after the initial injection and then gradually decreased, returning to control values by the end of the sixth month. Western blot experiments demonstrated that the immune sera specifically recognized DE in epididymal sperm extracts and epididymal cytosol, while no reaction was observed with different reproductive and essential organs. The immune sera were also capable of recognizing DE on the surface of both fresh and capacitated sperm as indicated by indirect immunofluorescence experiments. Finally, the exposure of sperm to immune sera prior to uterine insemination resulted in a significant (P < 0.05) reduction in the percentage of fertilized eggs compared to controls, with no effect on sperm motility and viability, nor on their ability to undergo capacitation. Together, these results support the participation of the raised antibodies as mediators of the antifertility effect and suggest a specific interference at the sperm-egg interaction level. © 1995.

Registro:

Documento: Artículo
Título:Potential contraceptive use of epididymal proteins: evidence for the participation of specific antibodies against rat epididymal protein DE in male and female fertility inhibition
Autor:Martinez, S.P.; Conesa, D.; Cuasnicú, P.S.
Filiación:Instituto de Biología y Medicina Experimental, Obligado 2490, 1428 Buenos Aires, Argentina
Palabras clave:Contraception; Epididymal proteins; Immunization; Spermatozoa; antibody; animal cell; animal experiment; animal tissue; article; artificial insemination; contraception; enzyme linked immunosorbent assay; epididymis; evolution; female; female fertility; immune response; immunization; immunoblotting; immunofluorescence; male; nonhuman; priority journal; rat; spermatozoon; spermatozoon capacitation; spermatozoon motility; Animal; Blotting, Western; Contraception, Immunologic; Epididymal Secretory Proteins; Epididymis; Female; Immune Sera; Immunization; Isoantibodies; Male; Metalloproteins; Rats; Rats, Inbred Lew; Rats, Wistar; Sperm Agglutination; Sperm Capacitation; Sperm Motility; Sperm-Ovum Interactions; Support, Non-U.S. Gov't; Testicular Hormones
Año:1995
Volumen:29
Número:1
Página de inicio:31
Página de fin:45
DOI: http://dx.doi.org/10.1016/0165-0378(95)00927-D
Título revista:Journal of Reproductive Immunology
Título revista abreviado:J. Reprod. Immunol.
ISSN:01650378
CODEN:JRIMD
CAS:Epididymal Secretory Proteins; Immune Sera; Isoantibodies; Metalloproteins; Testicular Hormones
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01650378_v29_n1_p31_Martinez

Referencias:

  • Allen, Bourne, Interaction of immunoglobulin fragments with the mammalian sperm acrosome (1978) J. Exp. Zool., 203, pp. 271-276
  • Bigazzi, Kosuda, Harnick, Sperm autoantibodies in vasectomized rats of different inbred strains (1977) Science, 197, pp. 1282-1283
  • Cuasnicú, Conesa, Rochwerger, Potential contraceptive use of an epididymal protein that participates in fertilization (1990) Gamete Interaction. Prospects for Immunocontraception, pp. 143-153. , N.J. Alexander, D. Griffin, J.M. Spieler, G.M.H. Waites, Wiley-Liss, New York
  • Cuasnicú, Rochwerger, Redistribution of a sperm epididymal protein after capacitation and its potential participation in sperm-egg fusion (1991) Comparative Spermatology: 20 Years After, 75, pp. 555-560. , B. Bachetti, Raven Press, New York
  • Cuasnicú, Gonzalez Echeverría, Piazza, Blaquier, Addition of androgens to cultured hamster epididymis increases zona recognition by immature spermatozoa (1984) J. Reprod. Fertil., 70, pp. 541-547
  • Cuasnicú, Gonzalez Echeverría, Piazza, Piñeiro, Blaquier, Epididymal proteins mimic the androgenic effect on zona pellucida recognition by immature hamster spermatozoa (1984) J. Reprod. Fertil., 71, pp. 427-431
  • Cuasnicú, Gonzalez Echeverría, Piazza, Cameo, Blaquier, Antibody against epididymal glicoproteins blocks fertilizing ability in rats (1984) J. Reprod. Fertil., 72, pp. 467-471
  • Friberg, A simple and sensitive micro-method for demonstration of sperm agglutinating activity in serum from infertile men and women (1974) Acta Obstetricia et Gynecologica Scandinavica, 36, p. 21. , (Suppl.)
  • Goldberg, Current status of research on sperm antigens: potential applications as contraceptive vaccines (1983) Research Frontiers in Fertility Regulation, 2, pp. 1-11. , G.I. Zatuchni, Program for Applied Research on Fertility Regulation, Chicago, IL, (6)
  • Gonzalez Echeverria, Cuasnicú, Piazza, Blaquier, Addition of an androgen-free epididymal protein extract increases the ability of immature hamster spermatozoa to fertilize in vivo and in vitro (1984) J. Reprod. Fertil., 71, pp. 433-437
  • Hames, An introduction to polyacrilamide gel electrophoresis (1981) Gel Electrophoresis of Proteins, pp. 1-88. , B.D. Hames, D. Rickwood, IRL Press, Oxford Washington DC
  • Herr, Flickinger, Howards, Scottyarbro, Spell, Caloras, Gallien, Temporal appearance of antisperm autoantibodies in Lewis rats following vasectomy (1987) J. Androl., 8, pp. 253-258
  • Kohane, Cameo, Piñeiro, Blaquier, Distribution and site of production of specific proteins in the rat epididymis (1980) Biol. Reprod., 23, pp. 181-187
  • Kohane, Gonzalez Echeverría, Piñeiro, Blaquier, Interaction of proteins of epididymal origin with spermatozoa (1980) Biol. Reprod., 23, pp. 737-742
  • Lee, Zhang, Wong, Chow, Yang, Leung, Sex difference of antifertility effect by passively immunized monoclonal sperm antibodies (1987) Am. J. Reprod. Immunol. Microbiol., 13, pp. 9-14
  • Mettler, Czuppon, Tinneberg, Immunization with spermatozoal peptides antigens resulting in immuno-suppression of fertility rates in female rats (1983) Andrología, 15, pp. 670-675
  • Moore, Glycoprotein secretions of the epididymis in the rabbit and hamster localization on epididymal spermatozoa and the effect of specific antibodies on fertilization in vivo (1981) Journal of Experimental Zoology, 2, pp. 77-85
  • Moore, Hartman, In vitro development of the fertilizing ability of hamster epididymal spermatozoa following co-culture with epithelium from proximal cauda epididymis (1986) J. Reprod. Fertil., 78, pp. 347-352
  • Naz, The fertilization antigen (FA-1) causes a reduction of fertility in actively immunized female rabbits (1987) J. Reprod. Immunol., 11, pp. 117-133
  • Naz, Bhargava, Antibodies to sperm surface fertilization antigen (FA-1): their specificities and the site of interaction with sperm in male genital tract (1990) Mol. Reprod. Dev., 26, pp. 175-183
  • Orgebin-Crist, Fournier Delpech, Sperm-egg interaction. Evidence for maturational changes during epididymal transit (1982) J. Androl., 3, pp. 429-433
  • Primakoff, Lathrop, Woolman, Cowan, Myles, Fully effective contraception in male and female guinea pigs immunized with the sperm protein PH-20 (1988) Nature, 335, pp. 543-546
  • Richards, Witkin, Non-immune IgG binding to the surface of spermatozoa by disulphide rearrangement (1984) Clin. Exp. Immunol., 58, pp. 493-501
  • Rochwerger, Cohen, Cuasnicú, Mammalian sperm-egg fusion: The rat egg has complementary sites for a sperm protein that mediates gamete fusion (1992) Dev. Biol., 153, pp. 83-90
  • Rochwerger, Cuasnicú, Redistribution of a rat sperm epididymal glycoprotein after in vitro and in vivo capacitation (1992) Mol. Reprod. Dev., 31, pp. 34-41
  • Rumke, Titus, Spermagglutinin formation in male rats by subcutaneously injected syngeneic epididymal spermatozoa and by vasoligation or vasectomy (1970) Reproduction, 21, pp. 69-70
  • Shaha, Suri, Talwar, Induction of infertility in female rats after active immunization with 24 kDa antigens from rat testes (1990) Int. J. Androl., 13, pp. 17-25
  • Swanson, Edwards, Young, Immunofluorescence technique and the isoantigeneicity of mammalian spermatozoa (1962) J. Reprod. Fertil., 4, pp. 103-111
  • Vanage, Lu, Tam, Koide, Infertility induced in rats by immunization with synthetic peptide segments of a sperm protein (1992) Biochem. Biophys. Res. Commun., 183, pp. 538-543
  • Weininger, Fisher, Rifkin, Bedford, Experimental studies on the passage of specific IgG to the lumen of the rabbit epididymis (1982) J. Reprod. Fertil., 66, pp. 251-258
  • Witkin, Shahani, Gupta, Good, Day, Demonstration of IgG Fc receptors on spermatozoa and their utilization for the detection of circulating immune complexes in human serum (1980) Clin. Exp. Immunol., 41, pp. 441-452

Citas:

---------- APA ----------
Martinez, S.P., Conesa, D. & Cuasnicú, P.S. (1995) . Potential contraceptive use of epididymal proteins: evidence for the participation of specific antibodies against rat epididymal protein DE in male and female fertility inhibition. Journal of Reproductive Immunology, 29(1), 31-45.
http://dx.doi.org/10.1016/0165-0378(95)00927-D
---------- CHICAGO ----------
Martinez, S.P., Conesa, D., Cuasnicú, P.S. "Potential contraceptive use of epididymal proteins: evidence for the participation of specific antibodies against rat epididymal protein DE in male and female fertility inhibition" . Journal of Reproductive Immunology 29, no. 1 (1995) : 31-45.
http://dx.doi.org/10.1016/0165-0378(95)00927-D
---------- MLA ----------
Martinez, S.P., Conesa, D., Cuasnicú, P.S. "Potential contraceptive use of epididymal proteins: evidence for the participation of specific antibodies against rat epididymal protein DE in male and female fertility inhibition" . Journal of Reproductive Immunology, vol. 29, no. 1, 1995, pp. 31-45.
http://dx.doi.org/10.1016/0165-0378(95)00927-D
---------- VANCOUVER ----------
Martinez, S.P., Conesa, D., Cuasnicú, P.S. Potential contraceptive use of epididymal proteins: evidence for the participation of specific antibodies against rat epididymal protein DE in male and female fertility inhibition. J. Reprod. Immunol. 1995;29(1):31-45.
http://dx.doi.org/10.1016/0165-0378(95)00927-D