Artículo
Paladini, A.A.; Erijman, L. "New methods for spectrofluorometer monochromator wavelength calibration" (1988) Journal of Biochemical and Biophysical Methods. 17(1):61-66
Estamos trabajando para incorporar este artículo al repositorio
Consulte la política de Acceso Abierto del editor
Abstract:
A method is presented for wavelength calibration of spectrofluorometer monochromators. It is based on the distortion that the characteristics absorption bands of glass filters (holmium or didymium oxide), commonly used for calibration of spectrophotometers, introduce in the emitted fluorescence of fluorophores like indole, diphenyl hexatriene, xylene or rhodamine 6G. Those filters or a well characterized absorber with sharp bands like benzene vapor can be used for the same purpose. The wavelength calibration accuracy obtained with this method is better than 0.1 nm, and requires no modification in the geometry of the spectrofluorometer sample compartment. © 1988.
Registro:
| Documento: | Artículo |
| Título: | New methods for spectrofluorometer monochromator wavelength calibration |
| Autor: | Paladini, A.A.; Erijman, L. |
| Filiación: | INGEBI (UBA, FCEN-CONICET), Obligado 2490, 1428 Buenos Aires, Argentina |
| Palabras clave: | Calibration; Didymium filter; Holmium filter; Instrumentation; Monochrmator; Wavelength; article; calibration; instrumentation; spectrofluorometry; standard; Calibration; Spectrometry, Fluorescence; Weights and Measures |
| Año: | 1988 |
| Volumen: | 17 |
| Número: | 1 |
| Página de inicio: | 61 |
| Página de fin: | 66 |
| DOI: | http://dx.doi.org/10.1016/0165-022X(88)90079-6 |
| Título revista: | Journal of Biochemical and Biophysical Methods |
| Título revista abreviado: | J. Biochem. Biophys. Methods |
| ISSN: | 0165022X |
| CODEN: | JBBMD |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0165022X_v17_n1_p61_Paladini |
Referencias:
- Miller, Techniques in Visible and UV Spectrometry, vol. 2: Standards in Fluorescence Spectrometry (1981) Monochromator Wavelength Calibrations, , Chapman & Hall, New York, ch. 2
- Mielenz, Optical Radiation Measurements, Vol. 3: Measurements of Photoluminescence, , Academic Press, New York
- Chen, (1967) Anal. Biochem., 20, pp. 339-357
- (1983) Annual Book of ASTM Standards, pp. 785-791. , ASTMA, Philadelphia, method E925-83
- McNeirney, Slavin, (1962) Applied Optics, 1, pp. 365-367
- Pye, (1969) Technical Manual, publ. No. 299184, (1). , Cambridge, England
- Friedel, Orchin, (1951) Ultraviolet Spectra of Aromatic Compounds, , John Wiley and Sons, New York
Citas:
---------- APA ----------
Paladini, A.A. & Erijman, L. (1988) . New methods for spectrofluorometer monochromator wavelength calibration. Journal of Biochemical and Biophysical Methods, 17(1), 61-66.http://dx.doi.org/10.1016/0165-022X(88)90079-6
---------- CHICAGO ----------
Paladini, A.A., Erijman, L. "New methods for spectrofluorometer monochromator wavelength calibration" . Journal of Biochemical and Biophysical Methods 17, no. 1 (1988) : 61-66.http://dx.doi.org/10.1016/0165-022X(88)90079-6
---------- MLA ----------
Paladini, A.A., Erijman, L. "New methods for spectrofluorometer monochromator wavelength calibration" . Journal of Biochemical and Biophysical Methods, vol. 17, no. 1, 1988, pp. 61-66.http://dx.doi.org/10.1016/0165-022X(88)90079-6
---------- VANCOUVER ----------
Paladini, A.A., Erijman, L. New methods for spectrofluorometer monochromator wavelength calibration. J. Biochem. Biophys. Methods. 1988;17(1):61-66.http://dx.doi.org/10.1016/0165-022X(88)90079-6
