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Pérez, G.; Garbossa, G.; Di Risio, C.; Vittori, D.; Nesse, A. "Disturbance of cellular iron uptake and utilisation by aluminium" (2001) Journal of Inorganic Biochemistry. 87(1):21-27
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Abstract:

Aluminium (Al) affects erythropoiesis but the real mechanism of action is still unknown. Transferrin receptors (TfR) in K562 cells are able to bind Tf, when carrying either iron (Fe) or Al, with similar affinity. Then, the aim of this work was to determine whether Al could interfere with the cellular Fe uptake and utilisation. K562 cells were induced to erythroid differentiation by either haemin (H) or sodium butyrate (B) and cultured with and without Al. The effect of Al on cellular Fe uptake, Fe incorporation to haem and cell differentiation was studied. H- and B-stimulated cells grown in the presence of 10 μM Al showed a reduction in the number of haemoglobinised cells (by 18% and 56%, respectively) and high amounts of Al content. Al2Tf inhibited both the 59Fe cellular uptake and its utilisation for haem synthesis. The removal of Al during the 59Fe pulse, after a previous incubation with the metal, allowed the cells to acquire Fe quantities in the normal range or even exceeding the amounts incorporated by the respective control cells. However, the Fe incorporated to haem could not reach control values in B-stimulated cells despite enough Fe acquisition was observed after removing Al. Present results suggest that Al might exert either reversible or irreversible effects on the haemoglobin synthesis depending on cellular conditions. © 2001 Elsevier Science B.V. All rights reserved.

Registro:

Documento: Artículo
Título:Disturbance of cellular iron uptake and utilisation by aluminium
Autor:Pérez, G.; Garbossa, G.; Di Risio, C.; Vittori, D.; Nesse, A.
Filiación:Laboratorio de Análisis Biológicos, Departamento de Química Biológica, Pabellón II, Piso 4, 1428 Buenos Aires, Argentina
Laboratorio de Radioquímica, Departamento de Química Inorgánica, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:Aluminium toxicity; Haem synthesis; Haemoglobin synthesis; Iron metabolism; K562 cell line; aluminum; butyric acid; heme; hemin; iron 59; transferrin receptor; binding affinity; cell count; cell culture; cell differentiation; cell growth; cell level; cell metabolism; cell stimulation; cell strain; conference paper; controlled study; erythroid cell; erythropoiesis; heme synthesis; hemoglobin synthesis; human; human cell; iron transport; receptor binding; Aluminum; Apoproteins; Barbiturates; Biological Transport; Cell Differentiation; Heme; Hemin; Hemoglobins; Humans; Iron; K562 Cells; Leukemia, Erythroblastic, Acute; Radioisotopes; Transferrin
Año:2001
Volumen:87
Número:1
Página de inicio:21
Página de fin:27
DOI: http://dx.doi.org/10.1016/S0162-0134(01)00310-5
Título revista:Journal of Inorganic Biochemistry
Título revista abreviado:J. Inorg. Biochem.
ISSN:01620134
CODEN:JIBID
CAS:Aluminum, 7429-90-5; Apoproteins; apotransferrin; Barbiturates; barbituric acid, 67-52-7; Heme, 14875-96-8; Hemin, 16009-13-5; Hemoglobins; Iron, 7439-89-6; Radioisotopes; Transferrin, 11096-37-0
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01620134_v87_n1_p21_Perez

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Citas:

---------- APA ----------
Pérez, G., Garbossa, G., Di Risio, C., Vittori, D. & Nesse, A. (2001) . Disturbance of cellular iron uptake and utilisation by aluminium. Journal of Inorganic Biochemistry, 87(1), 21-27.
http://dx.doi.org/10.1016/S0162-0134(01)00310-5
---------- CHICAGO ----------
Pérez, G., Garbossa, G., Di Risio, C., Vittori, D., Nesse, A. "Disturbance of cellular iron uptake and utilisation by aluminium" . Journal of Inorganic Biochemistry 87, no. 1 (2001) : 21-27.
http://dx.doi.org/10.1016/S0162-0134(01)00310-5
---------- MLA ----------
Pérez, G., Garbossa, G., Di Risio, C., Vittori, D., Nesse, A. "Disturbance of cellular iron uptake and utilisation by aluminium" . Journal of Inorganic Biochemistry, vol. 87, no. 1, 2001, pp. 21-27.
http://dx.doi.org/10.1016/S0162-0134(01)00310-5
---------- VANCOUVER ----------
Pérez, G., Garbossa, G., Di Risio, C., Vittori, D., Nesse, A. Disturbance of cellular iron uptake and utilisation by aluminium. J. Inorg. Biochem. 2001;87(1):21-27.
http://dx.doi.org/10.1016/S0162-0134(01)00310-5