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Abstract:

Species of Polybetes are known exclusively from South America. Currently there are 13 described species, 9 occurring in Argentina. Cytogenetic studies in spiders are scarce; the cytogenetics of only about 1% of nearly 39,500 described species are known. Within the Sparassidae, 38 species out of 1,009 (< 4%) have been cytogenetically analyzed; the most frequent chromosome number is 2n = 43/46 (male/female), n = 20 + X1X2X 3, present in almost half of the species studied. Female diploid chromosome number is only known for four species: Heteropoda venatoria (Linnaeus 1767) (2n = 44); Pediana regina (L. Koch 1875), Isopeda sp. and Olios sp. (2n = 46). Within the genus Polybetes, only P. pythagoricus (Holmberg 1875) had been previuosly cytogenetically analyzed. In the present work, the karyotype, heterochromatin content and distribution, and silver stained nucleolus-organizer regions of P. pythagoricus, P. rapidus (Keyserling 1880) and P. punctulatus Mello-Leitão 1944 are described and compared. In P. pythagoricus the identification of the chromosome pairs by means of G-banding is also performed. Females of the three species show a chromosome complement of 44 telocentric chromosomes, with a similar karyotype. Males of P. pythagoricus show 42 telocentric chromosomes, the two sex chromosomes being the largest and of different size. In the three species, two pairs of telomeric NORs and small pericentromeric positive C-bands in all chromosomes were detected. This C-banding pattern seems to be characteristic of spiders. Comparative analysis of chromosome complements in Sparassidae indicates that 2n = 42/44 (X 1X20/X1X1X2X 2) (male/female) may represent the ancestral karyotype for Polybetes.

Registro:

Documento: Artículo
Título:Cytogenetics in three species of Polybetes Simon 1897 from Argentina (Araneae, sparassidae) I. Karyotype and chromosome banding pattern
Autor:Rodríguez-Gil, S.G.; Merani, M.S.; Scioscia, C.L.; Mola, L.M.
Filiación:Departamento de Ecología, Genética y Evolución, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Autonoma de Buenos Aires, Argentina
Centro de Investigaciones en Reproducción, Facultad de Medicina, Universidad de Buenos Aires, Paraguay 2155, Ciudad Autonoma de Buenos Aires, Argentina
División Aracnología, Museo Argentino de Ciencias Naturales Bernardino Rivadavia, Av. Ángel Gallardo 470, Ciudad Autonoma de Buenos Aires, Argentina
Palabras clave:Chromosome number; Heterochromatin; Nucleolus-organizing regions; Telocentric chromosomes; Araneae; Heteropoda venatoria; Olios; Sparassidae
Año:2007
Volumen:35
Número:2
Página de inicio:227
Página de fin:237
DOI: http://dx.doi.org/10.1636/S05-69.1
Título revista:Journal of Arachnology
Título revista abreviado:J. Arachnol.
ISSN:01618202
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01618202_v35_n2_p227_RodriguezGil

Referencias:

  • Appels, R., Morris, R., Gill, B.S., May, C.E., (1998) Chromosome Biology, , Kluwer Academic Publishers, Boston, Massachusetts. 401 pp
  • Baldanza, F., Gaudio, L., Viggiani, G., Cytotaxonomic studies of Encarsia Förster (Hymenoptera: Aphelinidae) (1999) Bulletin of Entomological Research, 89, pp. 209-215
  • Benavente, R., Wettstein, R., Ultrastructural cytogenetics of the sex determination mechanisms of araneids (1978) Revista de Microscopía Electrónica, 5, pp. 320-321
  • Bole-Gowda, B.N., Studies on the chromosomes and the sex-determining system in four hunting spiders (Sparassidae) (1952) Proceedings of the Zoological Society, 5, pp. 51-70. , Bengal
  • Brum-Zorrilla, N., Postiglioni, A., Kariological studies on Uruguayan spiders. I. Banding pattern in chromosomes of Lycosa species (Araneae-Lycosidae) (1980) Genetica, 54, pp. 149-153
  • Brum-Zorrilla, N., Cazenave, A.M., Heterochromatin localization in the chromosomes of Lycosa malitiosa (Arachnida) (1974) Experientia, 30, pp. 94-95
  • Datta, S.N., Chatterjee, K., Chromosome number and sex-determining system in fifty-two species of spiders for north-east India (1983) Chromosome Information Service, 35, pp. 6-8
  • Datta, S.N., Chatterjee, K., Chromosome and sex determination in 13 araneid spiders of North-Eastern India (1988) Genetica, 76, pp. 91-99
  • De Araujo, D., Cella, D.M., Brescovit, A.D., Cytogenetic analysis of the neotropical spider Nephilengys cruentala (Araneomorphae, Tetragnathidae): Standard staining, Nors, C-Bands and base-specific fluorochromes (2005) Brazilian Journal of Biology, 65, pp. 193-202
  • De Araujo, D., Cella, D.M., Brescovit, A.D., Variabilidade cromossômica em très espécies de Scytodes Latreille, 1804 (Araneae, Haplogynae, Scytodidae) (2005) Primer Congreso Latinoamericano de Aracnología (Minas, p. 107. , Uruguay Actas
  • Díaz, M., Sáez, F.A., (1966) Karyotypes of South American Araneida, 33, pp. 153-154. , Memórias do Instituto Butantán Commemorativo
  • Díaz, M., Sáez, F.A., Investigaciones citogenéticas sobre algunas especies de araneidos uruguayos (1966) Anales (II) Congreso Latinoamericano de Zoología, pp. 3-9. , San Pablo
  • Gorlova, O.Y., Gorlov, J.P., Nevo, E., Logunov, D.V., Cytogenetic studies on seventeen spider species from Israel (1997) Bulletin of the British Arachnological Society, 10, pp. 249-252
  • Hackman, W., Chromosomenstudien an Araneen mit besonderer Berücksichtigung der Gechlechtschromosomen (1948) Acta Zoologica Fennica, 54, pp. 1-101
  • Hancock, A.J., Rowell, D.M., A chromosomal hybrid zone in the Australian huntsman spider, Delena cancerides (Araneae: Sparassidae). Evidence for a hybrid zone near Canberra, Australia (1995) Australian Journal of Zoology, 43, pp. 173-180
  • Howell, W.M., Black, D.A., Controlled silver staining of nucleolus organizer regions with a protective colloidal developer: A 1-step method (1980) Experientia, 36, pp. 1014-1015
  • Lorite, P., Chica, E., Palomeque, T., G-banding and chromosome condensation in the ant, Tapinoma nigerrimum (1996) Chromosome Research, 4, pp. 77-79
  • Mittal, O.P. 1961. Chromosome number and sex mechanism in twenty-one species of the Indian spiders. Research Bulletin (N. S.) of the Panjab University 12:271-273; Mittal, O.P., Karyological studies on Indian spiders. IV. Chromosomes in relation to taxonomy in Eusprassidae, Selenopidae and Thomisidae (1966) Genetica, 37, pp. 225-234
  • Olivera, G. 1978. Estudio citogenetico en Polybetes pythagoricus (Araneomorphae-Sparassidae). XX. Semana Univisitaria Gaucha de Debates Bioló gicos, Porto Allegre 24(Abstracts):251-253; Parida, B.B., Sharma, G.P., Karyotype and spermatogonia in an Indian hunting spider, Sparassus sp. (Sparassidae: Arachnida) with multiple sex chromosomes (1986) Chromosome Information Service, 40, pp. 28-30
  • Parida, B.B., Sharma, G.P., Chromosome number, sex mechanism and genome size in twenty seven species of Indian spiders (1987) Chromosome Information Service, 43, pp. 11-13
  • http://research.amnh.org/ entomology/spiders/catalog/index.html, Platnick, N.I. 2006. The World Spider Catalog, version 7.0. American Museum of Natural History, New York. Online at; http://www.colostate.edu/Depts/ Biology/Micromeasure, Reeves, A. & J. Tear. 2000. Micromeasure for Windows. Version 3.3 free program distributed by the author from; Rodrigrave;guez-Gil, S.G. 2006. Citogenética de arañas sinantrópicas de Argentina (Arachnida: Araneae). Ph. D. Thesis. Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires. 155 pp; Rowell, D.M., Complex sex-linked fusion heterozygosity in the Australian huntsman spider Delena cancerides (Araneae: Sparassidae) (1985) Chromosoma (Berlin), 93, pp. 169-176
  • Rowell, D.M., Fixed fusion heterozygosity in Delena cancerides Walck. (Araneae: Sparassidae): an alternative to speciation by monobranchial fusion (1990) Genetica, 80, pp. 139-157
  • Rowell, D.M., Chromosomal fusion and meiotic behaviour in Delena cancerides (Araneae: Sparassidae). I. Pairing behaviour and X-chromosome segregation (1991) Genome, 34, pp. 561-566
  • Rowell, D.M., Chromosomal fusion and meiotic behaviour in Delena cancerides (Araneae: Sparassidae). II. Chiasma position and its implications for speciation (1991) Genome, 34, pp. 567-573
  • Silva, R.W., D.D.R. Klisiowicz, D.M. Cella, O.C. Mangili & I.J. Sbalqueiro. 2002. Differential distribution of constitutive heterochromatin in two species of brown spider: Loxosceles intermedia and L. laeta (Araneae, Sicariidae), from the metropolitan region of Curitiba, PR (Brazil). Acta Biológica Paranaense, Curitiba 31:123-136; Srivastava, S.C., Shukla, S., Chromosome number and sex determining mechanism in forty-seven species of Indian spiders (1986) Chromosome Information Service, 41, pp. 23-26
  • Sumner, A.T., A simple technique for demonstrating centromeric heterochromatin (1972) Experimental Cell Research, 75, pp. 304-305
  • Sumner, A.T., (2003) Chromosomes: Organization and Function, , Blackwell Publishing, Oxford, UK. 287 pp
  • Suzuki, S., Sex determining mechanism and karyotypes in spiders (1950) Zoological Magazine (Tokyo), 59, pp. 31-32
  • Suzuki, S., Cytological Studies in spiders II. Chromosomal investigation in the twenty two species of spiders belonging to the four families, Clubionidae, Sparassidae, Thomisidae, and Oxyopidae, which constitute Clubionoidea, with special reference to sex chromosomes (1952) Journal of Science of the Hiroshima University, Series B, Division, 1, pp. 1-52. , 13
  • Suzuki, S., Okada, A., A study on the chromosomes of a spider, Heteropoda venatoria, with special reference to X1-, X2- and X3- chromosomes (1950) Series B. Journal of Science of the Hiroshima University, 1, pp. 1-47. , Division, 11

Citas:

---------- APA ----------
Rodríguez-Gil, S.G., Merani, M.S., Scioscia, C.L. & Mola, L.M. (2007) . Cytogenetics in three species of Polybetes Simon 1897 from Argentina (Araneae, sparassidae) I. Karyotype and chromosome banding pattern. Journal of Arachnology, 35(2), 227-237.
http://dx.doi.org/10.1636/S05-69.1
---------- CHICAGO ----------
Rodríguez-Gil, S.G., Merani, M.S., Scioscia, C.L., Mola, L.M. "Cytogenetics in three species of Polybetes Simon 1897 from Argentina (Araneae, sparassidae) I. Karyotype and chromosome banding pattern" . Journal of Arachnology 35, no. 2 (2007) : 227-237.
http://dx.doi.org/10.1636/S05-69.1
---------- MLA ----------
Rodríguez-Gil, S.G., Merani, M.S., Scioscia, C.L., Mola, L.M. "Cytogenetics in three species of Polybetes Simon 1897 from Argentina (Araneae, sparassidae) I. Karyotype and chromosome banding pattern" . Journal of Arachnology, vol. 35, no. 2, 2007, pp. 227-237.
http://dx.doi.org/10.1636/S05-69.1
---------- VANCOUVER ----------
Rodríguez-Gil, S.G., Merani, M.S., Scioscia, C.L., Mola, L.M. Cytogenetics in three species of Polybetes Simon 1897 from Argentina (Araneae, sparassidae) I. Karyotype and chromosome banding pattern. J. Arachnol. 2007;35(2):227-237.
http://dx.doi.org/10.1636/S05-69.1