Artículo

Toscano, M.A.; Campagna, L.; Molinero, L.L.; Cerliani, J.P.; Croci, D.O.; Ilarregui, J.M.; Fuertes, M.B.; Nojek, I.M.; Fededa, J.P.; Zwirner, N.W.; Costas, M.A.; Rabinovich, G.A. "Nuclear factor (NF)-κB controls expression of the immunoregulatory glycan-binding protein galectin-1" (2011) Molecular Immunology. 48(15-16):1940-1949
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Abstract:

The inflammatory response is a self-limiting process which involves the sequential activation of signaling pathways leading to the production of both pro- and anti-inflammatory mediators. Galectin-1 (Gal-1), an endogenous lectin found in peripheral lymphoid organs and inflammatory sites, elicits a broad spectrum of biological functions predominantly by acting as a potent anti-inflammatory factor and as a suppressive agent for T-cell responses. However, the molecular pathways underlying Gal-1 expression and function remain poorly understood. Here we identified a regulatory loop linking Gal-1 expression and function to NF-κB activation. NF-κB-activating stimuli increased Gal-1 expression on T cells, an effect which could be selectively prevented by inhibitors of NF-κB signaling. Accordingly, transient transfection of the p65 subunit of NF-κB was sufficient to induce high Gal-1 expression. Using in silico studies and chromatin immunoprecipitation analysis we have identified a functional NF-κB binding site within the first intron of the LGALS1 gene. In addition, our results show that exogenous Gal-1 can attenuate NF-κB activation, as shown by inhibition of IκB-α degradation induced by pro-inflammatory stimuli, higher cytoplasmic retention of p65, lower NF-κB DNA binding activity and impaired transcriptional activation of target genes. The present study suggest a novel regulatory loop by which NF-κB induces expression of Gal-1, which in turn may lead to negative control of NF-κB signaling. © 2011 Elsevier Ltd.

Registro:

Documento: Artículo
Título:Nuclear factor (NF)-κB controls expression of the immunoregulatory glycan-binding protein galectin-1
Autor:Toscano, M.A.; Campagna, L.; Molinero, L.L.; Cerliani, J.P.; Croci, D.O.; Ilarregui, J.M.; Fuertes, M.B.; Nojek, I.M.; Fededa, J.P.; Zwirner, N.W.; Costas, M.A.; Rabinovich, G.A.
Filiación:Laboratorio de Inmunopatología, Instituto de Biología y Medicina Experimental, Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET), Buenos Aires, Argentina
Laboratorio de Inmunogenética, Hospital de Clínicas José de San Martín, Universidad de Buenos Aires, Buenos Aires, Argentina
Laboratorio de Biología Molecular y Apoptosis, Instituto de Investigaciones Médicas Alfredo Lanari (IDIM), Consejo Nacional de Investigaciones Cientificasy Tecnologicas (CONICET), Universidad de Buenos Aires, Buenos Aires, Argentina
Departamento de Fisiología, Biología Molecular y Celular, IFIBYNE-UBA Consejo Nacional de Investigaciones Cientificas y Técnicas of Argentina, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Departamento de Microbiología, Parasitología e Inmunología, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Museo Argentino de Ciencias Naturales Bernardino Rivadavia, Av. Ángel Gallardo 470, C1405DJR, Buenos Aires, Argentina
Department of Medicine, The University of Chicago, MC0930, Chicago, IL 60637, United States
Institute of Biochemistry, Swiss Federal Institute of Technology Zurich (ETHZ), Schafmattstrasse 18, CH-8093 Zurich, Switzerland
Palabras clave:Galectin-1; Inflammation; NF-κB; T cells; galectin 1; glycan; I kappa B alpha; immunoglobulin enhancer binding protein; protein subunit; synaptotagmin I; tacrolimus; tumor necrosis factor alpha; article; binding site; cell differentiation; chromatin immunoprecipitation; chromosome 22; controlled study; cytoplasm; DNA binding; down regulation; gene targeting; genetic transfection; human; human cell; immunoregulation; inflammation; inhibition kinetics; intron; peripheral blood mononuclear cell; priority journal; protein analysis; protein degradation; protein expression; protein function; protein protein interaction; signal transduction; Th1 cell; Th2 cell; transcription initiation; transcription initiation site; Western blotting; Binding Sites; Blotting, Western; Cells, Cultured; Chromatin Immunoprecipitation; Electrophoretic Mobility Shift Assay; Feedback, Physiological; Galectin 1; Gene Expression; Gene Expression Regulation; Humans; Microscopy, Confocal; NF-kappa B; Reverse Transcriptase Polymerase Chain Reaction; Signal Transduction; T-Lymphocytes; Transfection
Año:2011
Volumen:48
Número:15-16
Página de inicio:1940
Página de fin:1949
DOI: http://dx.doi.org/10.1016/j.molimm.2011.05.021
Título revista:Molecular Immunology
Título revista abreviado:Mol. Immunol.
ISSN:01615890
CODEN:IMCHA
CAS:I kappa B alpha, 151217-48-0; galectin 1, 258495-34-0; tacrolimus, 104987-11-3; Galectin 1; LGALS1 protein, human; NF-kappa B
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01615890_v48_n15-16_p1940_Toscano

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Citas:

---------- APA ----------
Toscano, M.A., Campagna, L., Molinero, L.L., Cerliani, J.P., Croci, D.O., Ilarregui, J.M., Fuertes, M.B.,..., Rabinovich, G.A. (2011) . Nuclear factor (NF)-κB controls expression of the immunoregulatory glycan-binding protein galectin-1. Molecular Immunology, 48(15-16), 1940-1949.
http://dx.doi.org/10.1016/j.molimm.2011.05.021
---------- CHICAGO ----------
Toscano, M.A., Campagna, L., Molinero, L.L., Cerliani, J.P., Croci, D.O., Ilarregui, J.M., et al. "Nuclear factor (NF)-κB controls expression of the immunoregulatory glycan-binding protein galectin-1" . Molecular Immunology 48, no. 15-16 (2011) : 1940-1949.
http://dx.doi.org/10.1016/j.molimm.2011.05.021
---------- MLA ----------
Toscano, M.A., Campagna, L., Molinero, L.L., Cerliani, J.P., Croci, D.O., Ilarregui, J.M., et al. "Nuclear factor (NF)-κB controls expression of the immunoregulatory glycan-binding protein galectin-1" . Molecular Immunology, vol. 48, no. 15-16, 2011, pp. 1940-1949.
http://dx.doi.org/10.1016/j.molimm.2011.05.021
---------- VANCOUVER ----------
Toscano, M.A., Campagna, L., Molinero, L.L., Cerliani, J.P., Croci, D.O., Ilarregui, J.M., et al. Nuclear factor (NF)-κB controls expression of the immunoregulatory glycan-binding protein galectin-1. Mol. Immunol. 2011;48(15-16):1940-1949.
http://dx.doi.org/10.1016/j.molimm.2011.05.021