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Abstract:

Previously we have demonstrated a molecular relationship between H-2 class I antigens and β adrenoceptors from cardiac tissue. Here we show this type of interaction taking place with β adrenoceptors from splenic cells and their purified membranes and the participation of cytoskeletal proteins in the phenomenon. Alloimmune, as well as anti-class I but not anti-class II, antibodies were able to inhibit in a competitive manner the binding of (-)-[3 H]dihydroalprenolol to splenic lymphocytes and their purified membranes, and to increase cyclic AMP levels in intact cells as a consequence of β adrenoceptor activation. Furthermore, colchicine (a microtubule disrupting drug), but not cytochalasine B (a microfilament disrupting drug), was able to abrogate alloimmune antibody inhibition over the β radioligand binding to its receptor on both intact splenocytes and their membranes. Alloantibody actions were significantly diminished by peripheral protein solubilization in purified spleen cell membranes. These data pointed indirectly to the participation of a colchicine binding protein in class I antigen and hormone-receptor associations. © 1989.

Registro:

Documento: Artículo
Título:Colchicine blocks β adrenoceptor and class I antigen-specific interactions
Autor:Cremaschi, G.; Genaro, A.M.; Sterin-Borda, L.
Filiación:Centre de Estudios Farmacológicos y de Principios Naturales, CONICET, Serrano 665, 1414 Buenos Aires, Argentina
Palabras clave:alloantibody; alloantigens; colchicine; cytoskeletal proteins; β adrenoceptor; alloantibody; beta adrenergic receptor; colchicine; cyclic amp; cytoskeleton protein; hormone receptor; immunoglobulin g; major histocompatibility antigen class 1; animal cell; animal experiment; biological model; cell culture; cytochemistry; heart; immunization; lymphocyte; mouse; nonhuman; priority journal; spleen cell; Animal; Binding, Competitive; Colchicine; Cyclic AMP; Cytochalasin B; H-2 Antigens; Immunoglobulin G; Isoantibodies; Mice; Mice, Inbred BALB C; Mice, Inbred C3H; Receptors, Adrenergic, beta; Spleen; Support, Non-U.S. Gov't
Año:1989
Volumen:26
Número:7
Página de inicio:601
Página de fin:609
DOI: http://dx.doi.org/10.1016/0161-5890(89)90042-4
Título revista:Molecular Immunology
Título revista abreviado:Mol. Immunol.
ISSN:01615890
CODEN:IMCHA
CAS:colchicine, 64-86-8; cyclic AMP, 60-92-4; immunoglobulin G, 97794-27-9; Colchicine, 64-86-8; Cyclic AMP, 60-92-4; Cytochalasin B, 14930-96-2; H-2 Antigens; Immunoglobulin G; Isoantibodies; Receptors, Adrenergic, beta
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01615890_v26_n7_p601_Cremaschi

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Citas:

---------- APA ----------
Cremaschi, G., Genaro, A.M. & Sterin-Borda, L. (1989) . Colchicine blocks β adrenoceptor and class I antigen-specific interactions. Molecular Immunology, 26(7), 601-609.
http://dx.doi.org/10.1016/0161-5890(89)90042-4
---------- CHICAGO ----------
Cremaschi, G., Genaro, A.M., Sterin-Borda, L. "Colchicine blocks β adrenoceptor and class I antigen-specific interactions" . Molecular Immunology 26, no. 7 (1989) : 601-609.
http://dx.doi.org/10.1016/0161-5890(89)90042-4
---------- MLA ----------
Cremaschi, G., Genaro, A.M., Sterin-Borda, L. "Colchicine blocks β adrenoceptor and class I antigen-specific interactions" . Molecular Immunology, vol. 26, no. 7, 1989, pp. 601-609.
http://dx.doi.org/10.1016/0161-5890(89)90042-4
---------- VANCOUVER ----------
Cremaschi, G., Genaro, A.M., Sterin-Borda, L. Colchicine blocks β adrenoceptor and class I antigen-specific interactions. Mol. Immunol. 1989;26(7):601-609.
http://dx.doi.org/10.1016/0161-5890(89)90042-4