Artículo
Couto, A.S.; Uhrig, M.L.; Agusti, R.; Befumo, M.F.; Zingales, B.; Colli, W.; De Lederkremer, R.M. "Trypanosoma cruzi's incorporation of [3H]-palmitic acid and [3H] -galactose into components shed by trypomastigotes" (1991) Biochemistry International. 24(6):991-1002
Estamos trabajando para incorporar este artículo al repositorio
Consulte la política de Acceso Abierto del editor
Abstract:
Trypomastigotes were metabolically labeled with [3H]-palmitic acid or [3H] -galactose and labeled components were detected in the culture medium. Thin layer chromatography of the shed material showed several lipids in the [3H] -palmitic acid labeled sample while the sugar was mainly incorporated into macromolecules. The material incorporated with the lipidic precursor was fractionated by DEAE-Sephadex (acetate form) and the amount of radioactivity was ten times higher in the acidic lipids than in the neutral lipids. When acidic lipids were further separated by Unisil, 73% of the radioactivity was recovered in the less polar fraction. Different patterns were obtained on comparison of the shed components with the lipids remaining in the parasite.
Registro:
| Documento: | Artículo |
| Título: | Trypanosoma cruzi's incorporation of [3H]-palmitic acid and [3H] -galactose into components shed by trypomastigotes |
| Autor: | Couto, A.S.; Uhrig, M.L.; Agusti, R.; Befumo, M.F.; Zingales, B.; Colli, W.; De Lederkremer, R.M. |
| Filiación: | Depto de Quimica Organica, Fac de Cienc Exac y Naturales, Universidad de Buenos Aires, Pabellon 11, Argentina |
| Palabras clave: | endogenous compound; galactose; glycoconjugate; lipid; membrane lipid; palmitic acid; parasite antigen; galactose; glycoconjugate; lipid; palmitic acid derivative; article; cell culture; cell surface; culture medium; macromolecule; membrane vesicle; nonhuman; ph; priority journal; thin layer chromatography; trypanosoma cruzi; trypomastigote; animal; chemistry; culture medium; fractionation; gel chromatography; metabolism; Trypanosoma cruzi; Trypanosoma; Trypanosoma cruzi; Animal; Chemical Fractionation; Chromatography, Gel; Chromatography, Thin Layer; Culture Media; Galactose; Glycoconjugates; Lipids; Palmitic Acid; Palmitic Acids; Support, Non-U.S. Gov't; Trypanosoma cruzi |
| Año: | 1991 |
| Volumen: | 24 |
| Número: | 6 |
| Página de inicio: | 991 |
| Página de fin: | 1002 |
| Título revista: | Biochemistry International |
| Título revista abreviado: | BIOCHEM. INT. |
| ISSN: | 01585231 |
| CODEN: | BIIND |
| CAS: | galactose, 26566-61-0, 50855-33-9, 59-23-4; lipid, 66455-18-3; palmitic acid, 57-10-3; Culture Media; Galactose, 26566-61-0; Glycoconjugates; Lipids; Palmitic Acid, 57-10-3; Palmitic Acids |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01585231_v24_n6_p991_Couto |
Citas:
---------- APA ----------
Couto, A.S., Uhrig, M.L., Agusti, R., Befumo, M.F., Zingales, B., Colli, W. & De Lederkremer, R.M. (1991) . Trypanosoma cruzi's incorporation of [3H]-palmitic acid and [3H] -galactose into components shed by trypomastigotes. Biochemistry International, 24(6), 991-1002.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01585231_v24_n6_p991_Couto [ ]
---------- CHICAGO ----------
Couto, A.S., Uhrig, M.L., Agusti, R., Befumo, M.F., Zingales, B., Colli, W., et al. "Trypanosoma cruzi's incorporation of [3H]-palmitic acid and [3H] -galactose into components shed by trypomastigotes" . Biochemistry International 24, no. 6 (1991) : 991-1002.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01585231_v24_n6_p991_Couto [ ]
---------- MLA ----------
Couto, A.S., Uhrig, M.L., Agusti, R., Befumo, M.F., Zingales, B., Colli, W., et al. "Trypanosoma cruzi's incorporation of [3H]-palmitic acid and [3H] -galactose into components shed by trypomastigotes" . Biochemistry International, vol. 24, no. 6, 1991, pp. 991-1002.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01585231_v24_n6_p991_Couto [ ]
---------- VANCOUVER ----------
Couto, A.S., Uhrig, M.L., Agusti, R., Befumo, M.F., Zingales, B., Colli, W., et al. Trypanosoma cruzi's incorporation of [3H]-palmitic acid and [3H] -galactose into components shed by trypomastigotes. BIOCHEM. INT. 1991;24(6):991-1002.Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01585231_v24_n6_p991_Couto [ ]
