Artículo
Mazzetti, M.B.; Tomio, J.M. "Kinetic and molecular parameters of human hepatic porphobilinogen deaminase" (1990) Biochemistry International. 21(3):463-471
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Abstract:
A basal level of human liver porphobilinogen deaminase of 3.66 units/g wet weight was found in adult tissue. Activity in neonatal liver was at least three fold higher. Physico-chemical studies revealed that the enzyme has the approximate form of a symmetrical molecule and exhibits hyperbolic kinetics with a Km value of 3.6 μM at pH 7.6. Two ionizable groups with pK values of 7.35 and 8.90 are prominent for catalysis. A set of pI (5.8 - 4.9) were observed under different conditions. Results demonstrate the existence of a single protein differentially charged with multiple molecular forms in adult liver.
Registro:
| Documento: | Artículo |
| Título: | Kinetic and molecular parameters of human hepatic porphobilinogen deaminase |
| Autor: | Mazzetti, M.B.; Tomio, J.M. |
| Filiación: | Departamento de Quimica Biologica, Facultae de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellon 2, Buenos Aires 1428, Argentina |
| Palabras clave: | liver enzyme; porphobilinogen deaminase; article; human; human cell; priority journal; Adult; Human; Hydrogen-Ion Concentration; Hydroxymethylbilane Synthase; Infant, Newborn; Isoelectric Focusing; Kinetics; Liver; Support, Non-U.S. Gov't |
| Año: | 1990 |
| Volumen: | 21 |
| Número: | 3 |
| Página de inicio: | 463 |
| Página de fin: | 471 |
| Título revista: | Biochemistry International |
| Título revista abreviado: | BIOCHEM. INT. |
| ISSN: | 01585231 |
| CODEN: | BIIND |
| CAS: | Hydroxymethylbilane Synthase, EC 4.3.1.8 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01585231_v21_n3_p463_Mazzetti |
Citas:
---------- APA ----------
Mazzetti, M.B. & Tomio, J.M. (1990) . Kinetic and molecular parameters of human hepatic porphobilinogen deaminase. Biochemistry International, 21(3), 463-471.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01585231_v21_n3_p463_Mazzetti [ ]
---------- CHICAGO ----------
Mazzetti, M.B., Tomio, J.M. "Kinetic and molecular parameters of human hepatic porphobilinogen deaminase" . Biochemistry International 21, no. 3 (1990) : 463-471.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01585231_v21_n3_p463_Mazzetti [ ]
---------- MLA ----------
Mazzetti, M.B., Tomio, J.M. "Kinetic and molecular parameters of human hepatic porphobilinogen deaminase" . Biochemistry International, vol. 21, no. 3, 1990, pp. 463-471.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01585231_v21_n3_p463_Mazzetti [ ]
---------- VANCOUVER ----------
Mazzetti, M.B., Tomio, J.M. Kinetic and molecular parameters of human hepatic porphobilinogen deaminase. BIOCHEM. INT. 1990;21(3):463-471.Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01585231_v21_n3_p463_Mazzetti [ ]
