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Abstract:

In order to search for early changes induced by the application of human immunoglobulin G (IgG) on motor nerve terminals, IgG from patients with amyotrophic lateral sclerosis (ALS) and control subjects was injected subcutaneously into the levator auris muscle of mice. A week or a month after the last injection, endplate potentials were recorded. No changes in quantal content of transmitter release were observed. In control and ALS IgG-treated muscles, neurotransmitter release remained sensitive to P/Q-type and insensitive to N-type voltage-sensitive calcium channel (VSCC) blockers as in untreated muscles. In contrast, IgG from 5 of 8 different ALS patients induced a significant reduction in quantal content of the evoked response after incubation with nitrendipine, indicating that a novel sensitivity to this calcium channel blocker appears in these motor nerve terminals. These results indicate that ALS IgG induces plastic changes at nerve terminals. The expression of transmitter release coupled to L-type VSCC indicate that ALS IgGs are capable of inducing plastic changes at the nerve terminals that may participate in the process leading to neuronal death. (C) 2000 John Wiley and Sons, Inc.

Registro:

Documento: Artículo
Título:Amyotrophic lateral sclerosis IgG-treated neuromuscular junctions develop sensitivity to L-type calcium channel blocker
Autor:Fratantoni, S.A.; Weisz, G.; Pardal, A.M.; Reisin, R.C.; Uchitel, O.D.
Filiación:Inst. Biol. Cel. y Neurociencias P., Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina
Lab. de Fisiol. y Biologia Molecular, Facultad de Ciencias Exactas, Universidad de Buenos Aires, Buenos Aires, Argentina
Hospital Británico, Buenos Aires, Argentina
Inst. Fisiologia y Biologia Molec., Facultad de Ciencias Exastas, Pab. II, 2 piso, Capital Federal 1428, Argentina
Palabras clave:Amyotrophic lateral sclerosis; Calcium channels; Neuromuscular junction; Nitrendipine; Transmitter release; calcium channel blocking agent; immunoglobulin G; nitrendipine; adult; aged; amyotrophic lateral sclerosis; animal cell; animal model; animal tissue; article; calcium transport; clinical article; controlled study; female; human; human cell; human tissue; immunotherapy; male; mouse; nerve cell necrosis; neurotransmitter release; nonhuman; priority journal; protein expression; voltage clamp; Adult; Aged; Animals; Calcium Channel Blockers; Calcium Channels, L-Type; Evoked Potentials; Female; Humans; Immunoglobulin G; Male; Mice; Middle Aged; Motor Neuron Disease; Muscle, Skeletal; Neuromuscular Junction; Nitrendipine; omega-Agatoxin IVA; omega-Conotoxin GVIA; Reference Values
Año:2000
Volumen:23
Número:4
Página de inicio:543
Página de fin:550
DOI: http://dx.doi.org/10.1002/(SICI)1097-4598(200004)23:4<543::AID-MUS13>3.0.CO;2-S
Título revista:Muscle and Nerve
Título revista abreviado:Muscle Nerve
ISSN:0148639X
CODEN:MUNED
CAS:Calcium Channel Blockers; Calcium Channels, L-Type; Immunoglobulin G; Nitrendipine, 39562-70-4; omega-Agatoxin IVA; omega-Conotoxin GVIA, 92078-76-7
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0148639X_v23_n4_p543_Fratantoni

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Citas:

---------- APA ----------
Fratantoni, S.A., Weisz, G., Pardal, A.M., Reisin, R.C. & Uchitel, O.D. (2000) . Amyotrophic lateral sclerosis IgG-treated neuromuscular junctions develop sensitivity to L-type calcium channel blocker. Muscle and Nerve, 23(4), 543-550.
http://dx.doi.org/10.1002/(SICI)1097-4598(200004)23:4<543::AID-MUS13>3.0.CO;2-S
---------- CHICAGO ----------
Fratantoni, S.A., Weisz, G., Pardal, A.M., Reisin, R.C., Uchitel, O.D. "Amyotrophic lateral sclerosis IgG-treated neuromuscular junctions develop sensitivity to L-type calcium channel blocker" . Muscle and Nerve 23, no. 4 (2000) : 543-550.
http://dx.doi.org/10.1002/(SICI)1097-4598(200004)23:4<543::AID-MUS13>3.0.CO;2-S
---------- MLA ----------
Fratantoni, S.A., Weisz, G., Pardal, A.M., Reisin, R.C., Uchitel, O.D. "Amyotrophic lateral sclerosis IgG-treated neuromuscular junctions develop sensitivity to L-type calcium channel blocker" . Muscle and Nerve, vol. 23, no. 4, 2000, pp. 543-550.
http://dx.doi.org/10.1002/(SICI)1097-4598(200004)23:4<543::AID-MUS13>3.0.CO;2-S
---------- VANCOUVER ----------
Fratantoni, S.A., Weisz, G., Pardal, A.M., Reisin, R.C., Uchitel, O.D. Amyotrophic lateral sclerosis IgG-treated neuromuscular junctions develop sensitivity to L-type calcium channel blocker. Muscle Nerve. 2000;23(4):543-550.
http://dx.doi.org/10.1002/(SICI)1097-4598(200004)23:4<543::AID-MUS13>3.0.CO;2-S