Artículo

Mateu, C.G.; Recalde, M.P.; Artuso, M.C.; Hermida, G.; Linero, F.N.; Scolaro, L.A.; Damonte, E.B.; Pujol, C.A.; Carlucci, M.J. "Emergence of herpes simplex virus-1 syncytial variants with altered virulence for mice after selection with a natural carrageenan" (2011) Sexually Transmitted Diseases. 38(6):555-561
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Abstract:

Background: Antiviral therapy against herpes simplex virus based on sulfated polysaccharides, like carrageenans, represents a new alternative for genital herpes infections treatment and arises the concern about the appearance of resistant viral populations. Methods: We characterized the F strain of herpes simplex virus-1 passaged in the presence of a natural carrageenan isolated from the red seaweed Gigartina skottbergii in view of the virulence for mice of isolated viral clones. Results: Viral clones (syn14-1 and syn17-2) showed a syncytial phenotype and a mild resistance to carrageenan, heparin, acyclovir, and brivudine. Both clones were avirulent for BALB/c mice when inoculated intravaginally, whereas F strain produced high mortality. Attenuation correlated with low levels of TNF-α, interleukin-6, and IFN-γ in vaginal lavages although virus titers were similar to those obtained for F strain. On the contrary, they showed a marked virulence when inoculated intranasally leading to a generalized spreading of virus. Conclusions: Results confirm the hypothesis that selection of herpes simplex virus-1 with a carrageenan in vitro leads to the emergence of variants with a differential virulence when compared to the original virus. This finding should be addressed when an antiviral therapy against genital herpes infection employing a natural carrageenan is under consideration. © 2011 American Sexually Transmitted Diseases Association All rights reserved.

Registro:

Documento: Artículo
Título:Emergence of herpes simplex virus-1 syncytial variants with altered virulence for mice after selection with a natural carrageenan
Autor:Mateu, C.G.; Recalde, M.P.; Artuso, M.C.; Hermida, G.; Linero, F.N.; Scolaro, L.A.; Damonte, E.B.; Pujol, C.A.; Carlucci, M.J.
Filiación:Departamento de Química Biológica, Laboratorio de Virologia, Universidad de Buenos Aires, Pab. 2, Piso 4, 1428EGA Buenos Aires, Argentina
Departamento de Química Orgánica-CIHIDECAR (CONICET-UBA), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Departamento de Biodiversidad y Biología Experimental, Laboratorio de Histología Animal, Universidad de Buenos Aires, Buenos Aires, Argentina
Palabras clave:5 (2 bromovinyl) 2' deoxyuridine; aciclovir; carrageenan; gamma interferon; heparin; interleukin 10; interleukin 1beta; interleukin 6; tumor necrosis factor alpha; animal tissue; antiviral susceptibility; article; Bagg albino mouse; controlled study; female; Gigartina skottbergii; Herpes simplex virus 1; IC 50; in vitro study; mouse; nonhuman; phenotype; seaweed; vaginal lavage; virus attenuation; virus isolation; virus resistance; virus strain; virus virulence; Animals; Antiviral Agents; Carrageenan; Cercopithecus aethiops; Female; Genetic Variation; Giant Cells; Herpes Genitalis; Herpes Simplex; Herpesvirus 1, Human; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Microbial Sensitivity Tests; Rhodophyta; Seaweed; Selection, Genetic; Vero Cells; Virulence
Año:2011
Volumen:38
Número:6
Página de inicio:555
Página de fin:561
DOI: http://dx.doi.org/10.1097/OLQ.0b013e3182084d99
Título revista:Sexually Transmitted Diseases
Título revista abreviado:Sex. Transm. Dis.
ISSN:01485717
CODEN:STRDD
CAS:5 (2 bromovinyl) 2' deoxyuridine, 69304-47-8, 82768-44-3; aciclovir, 59277-89-3; carrageenan, 9000-07-1, 9049-05-2, 9061-82-9, 9064-57-7; gamma interferon, 82115-62-6; heparin, 37187-54-5, 8057-48-5, 8065-01-8, 9005-48-5; Antiviral Agents; Carrageenan, 9000-07-1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01485717_v38_n6_p555_Mateu

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Citas:

---------- APA ----------
Mateu, C.G., Recalde, M.P., Artuso, M.C., Hermida, G., Linero, F.N., Scolaro, L.A., Damonte, E.B.,..., Carlucci, M.J. (2011) . Emergence of herpes simplex virus-1 syncytial variants with altered virulence for mice after selection with a natural carrageenan. Sexually Transmitted Diseases, 38(6), 555-561.
http://dx.doi.org/10.1097/OLQ.0b013e3182084d99
---------- CHICAGO ----------
Mateu, C.G., Recalde, M.P., Artuso, M.C., Hermida, G., Linero, F.N., Scolaro, L.A., et al. "Emergence of herpes simplex virus-1 syncytial variants with altered virulence for mice after selection with a natural carrageenan" . Sexually Transmitted Diseases 38, no. 6 (2011) : 555-561.
http://dx.doi.org/10.1097/OLQ.0b013e3182084d99
---------- MLA ----------
Mateu, C.G., Recalde, M.P., Artuso, M.C., Hermida, G., Linero, F.N., Scolaro, L.A., et al. "Emergence of herpes simplex virus-1 syncytial variants with altered virulence for mice after selection with a natural carrageenan" . Sexually Transmitted Diseases, vol. 38, no. 6, 2011, pp. 555-561.
http://dx.doi.org/10.1097/OLQ.0b013e3182084d99
---------- VANCOUVER ----------
Mateu, C.G., Recalde, M.P., Artuso, M.C., Hermida, G., Linero, F.N., Scolaro, L.A., et al. Emergence of herpes simplex virus-1 syncytial variants with altered virulence for mice after selection with a natural carrageenan. Sex. Transm. Dis. 2011;38(6):555-561.
http://dx.doi.org/10.1097/OLQ.0b013e3182084d99