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Abstract:

Long-term pregestational ethanol exposure induced altered fertilization and preimplantation embryogenesis. We evaluated preimplantational embryo-trophoblast differentiation, growth and invasiveness after perigestational ethanol 10% ingestion for 15 days preceding and up to day 4 (treated females [TF]: TF-D4 group) or 5 (TF-D5) of CD-1 gestation (control females [CF] with water). In TF-D4, expanded and hatched blastocyst numbers were significantly reduced (p<0.05) versus CF-D4. Abnormal embryos and percentage of pyknotic nuclei were increased, and early blastocyst growth (nuclear number/embryo) and mitotic index was reduced (p<0.05) versus CF-D4. On day 5 of gestation, TF-D5 presented significantly reduced total embryos and advanced embryo type 3 number versus CF-D5 (p<0.05). During in vitro development, up to 72-hour culture, TF-D5 had reduced embryo type 1 (the least developed) and 3 percentages (p<0.05) versus controls, whereas embryo type 2 percentage increased (p<0.05) versus CF-D5. Embryo-trophoblast growth was studied during culture by morphometry. Embryo size ranges were classified as small, medium and large embryos. At 48-hour culture, small and medium embryos of TF had significantly increased mean area versus CF (p<0.05), whereas large embryos had reduced mean area at 24-hour culture. Perigestational alcohol exposure up to days 4-5 induced embryo differentiation retardation, abnormal blastocyst growth and alterations of embryo-trophoblast growth and expansion during implantation, suggesting impaired regulation of trophoblast invasion and a relation with early pregnancy loss after mouse perigestational alcohol consumption. © 2014 Informa Healthcare USA, Inc. All rights reserved: reproduction in whole or part not permitted.

Registro:

Documento: Artículo
Título:Peri-implantational in vivo and in vitro embryo-trophoblast development after perigestational alcohol exposure in the CD-1 mouse
Autor:Pérez-Tito, L.; Bevilacqua, E.; Cebral, E.
Filiación:Laboratorio de Reproducción y Fisiopatología Materno-Embrionaria, Instituto de Fisiología, Biología SMolecular y Neurociencias (IFIBYNE-CONICET), Buenos Aires, Argentina
Departamento de Biodiversidad y Biología Experimental (DBBE), Facultad de Ciencias Exactas y Naturales (FCEyN), Universidad de Buenos Aires (UBA), Buenos Aires, Argentina
Department of Cell and Developmental Biology, Institute of Biomedical Science, University of Sao Paulo (USP), Sao Paulo, Brazil
Palabras clave:Alcohol; Blastocyst; Differentiation; Growth; Mouse; Trophoblast; alcohol; animal experiment; article; blastocyst; body weight; caloric intake; cell differentiation; cell division; cell expansion; cell growth; cell invasion; controlled study; embryo; embryo development; embryo growth; female; fluid intake; food intake; in vitro study; in vivo study; inner cell mass; micronucleus; mitosis index; morphology; mouse; nidation; nonhuman; preimplantation embryo; prenatal exposure; trophoblast; Alcohol Drinking; Animals; Blastocyst; Embryo Implantation; Embryo, Mammalian; Embryonic Development; Female; Fertilization; Maternal Exposure; Mice; Pregnancy; Time Factors; Trophoblasts
Año:2014
Volumen:37
Número:2
Página de inicio:184
Página de fin:197
DOI: http://dx.doi.org/10.3109/01480545.2013.834358
Título revista:Drug and Chemical Toxicology
Título revista abreviado:Drug Chem. Toxicol.
ISSN:01480545
CODEN:DCTOD
CAS:alcohol, 64-17-5
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01480545_v37_n2_p184_PerezTito

Referencias:

  • Antezak, M., Van Blerkom, J., Oocyte influence on early development: The regulatory proteins leptin and Stat-3 proteins are polarised in mouse and human oocytes and differentially distributed within the cells of the pre-implantation embryo (1997) Mol Human Reprod, 3, pp. 1067-1086
  • Astley, S.J., Fetal alcohol syndrome prevention in Washington State: Evidence of success (2004) Paediatr Perinat Epidemiol, 18, pp. 344-351
  • Bevilacqua, E.M., Abrahamsohn, P.A., Ultrastructure of trophoblast giant cell transformation during the invasive stage of implantation of the mouse embryo (1988) J Morphol, 198, pp. 341-351
  • Brison, D.R., Leese, H.J., The role of exogenous energy substrates in blastocoele fluid accumulation in the rat (1994) Zygote, 2, pp. 69-77
  • Cebral, E., Faletti, A.B., Jawerbaum, A., Paz, D.A., Periconceptional alcohol consumption-induced changes in embryonic prostaglandin e levels in mouse organogenesis Modulation by nitric oxide (2007) Prostaglandins Leukot Essent Fatty Acids, 76, pp. 141-151
  • Cebral, E., Lasserre, A., Motta, A., Maf, G., Mouse oocyte quality and prostaglandin synthesis by cumulus oocyte complex after moderate chronic ethanol intake (1998) Prostaglandins Leukot Essent Fatty Acids, 58, pp. 381-387
  • Cebral, E., Lasserre, A., Rettori, V., Maf, G., Alterations in preimplantation in vivo development alter preconceptional chronic moderate alcohol consumption in female mice (2000) Alcohol Alcohol, 35, pp. 336-343
  • Cebral, E., Rettori, V., Gimeno, M.A., Impact of chronic low-dose alcohol ingestion during sexual maturation of female mice on in-vitro and in-vivo embryo development (2001) Reprod Toxicol, 15, pp. 123-129
  • Cebral, E., Lasserre, A., Rettori, V., Deleterious effects of chronic moderate alcohol intake by female mice on preimplantation embryo growth in vitro (1999) Alcohol Alcohol, 34, pp. 551-558
  • Cebral, E., Lasserre, A., Rettori, V., Impaired mouse fertilization by low chronic alcohol treatment (1997) Alcohol Alcohol, 32, pp. 563-572
  • Cebral, E., Motta, A., Moquet, M., Effects of low chronic ethanol exposure on prostaglandin e synthesis by preimplantation mouse embryos (1998) Prostaglandins Leukot Essent Fatty Acids, 58, pp. 249-255
  • Coll, T.A., Perez-Tito, L., Cma, S., Cebral, E., Embryo developmental disruption during organogenesis produced by CF-1 murine periconceptional alcohol consumption (2011) Birth Defects Res B, 92, pp. 560-574
  • Hard, M.L., Einarson, T.R., Koren, G., The role of acetaldehyde in pregnancy outcome after prenatal alcohol exposure (2001) Theor Drug Monit, 4, pp. 427-434
  • Hardy, K., Winston, R.M., Handyside, A.H., Binucleate blastomeres in preimplantation human embryos in vitro: Failure of cytokinesis during early cleavage (1993) J Reprod Fertil, 98, pp. 549-558
  • Haycock, P.C., Ramsay, M., Exposure of mouse embryos to ethanol during preimplantation development: Effect on DNA methylation in the H19 imprinting control region (2009) Biol Reprod, 81, pp. 618-627
  • Hoyme, H.E., May, P.A., Kalberg, W.O., A practical clinical approach to diagnosis of fetal alcohol spectrum disorders: Clarification of the 1996 Institute of Medicine criteria (2005) Pediatrics, 115, pp. 39-47
  • Hu, D., Cross, J.C., Development and function of trophoblast giant cells in the rodent placenta (2010) Int J Dev Biol, 54, pp. 341-354
  • Kalmus, G.W., Buckenmaier, C.C., Effects of ethanol and acetaldehyde on cultured pre-implantation mouse embryos (1989) Experientia, 45, pp. 484-487
  • Kaufman, M.H., (1990) Early Mammalian Development: Parthenogenetic Studies, pp. 144-145. , Cambridge, UK: Cambridge University Press
  • Kaufman, M.H., The teratogenic effects of alcohol following exposure during pregnancy and its influence on the chromosome constitution of pre-ovulatory egg (1997) Alcohol Alcohol, 32, pp. 113-128
  • Kennedy, T.G., Evidence for a role for prostaglandins in the initiation of blastocyst implantation in the rat (1977) Biol Reprod, 16, pp. 286-291
  • Khong, T.Y., De Wolf, F., Robertson, W.B., Brosens, I., Inadequate maternal vascular response to placentation in pregnancies complicated by pre-eclampsia and by small-for-gestational age infants (1986) Br J Obstet Gynaec, 93, pp. 1049-1059
  • Khong, T.Y., Liddell, H.S., Robertson, W.B., Defective haemochorial placentation as a cause of miscarriage: A preliminary study (1987) Br J Obstet Gynaecol, 94, pp. 649-655
  • Kowalczyk, J.J., Stachecki, J.F., Schultz, R.E., Effects of alcohols on murine preimplantation development: Relationship to relative membrane disordering potency (1996) Alcohol Clin Exp Res, 20, pp. 566-571
  • Lau, C.F., Vogel, R., Obe, G., Spielmann, H., Embryologic and cytogenetic effects of ethanol on preimplantation mouse embryos in vitro (1991) Reprod Toxicol, 5, pp. 405-410
  • Leach, R.E., Stachecki, J.J., Randall, A., Development of in vitro fertilized mouse embryos exposed to ethanol during the preimplantation period: Accelerated embryogenesis at subtoxic levels (1993) Teratology, 47, pp. 57-64
  • Musacchio, A., Hardwick, K.G., The spindle checkpoint: Structural insights into dynamic signaling (2002) Nat Rev Mol Cell Biol, 3, pp. 731-741
  • Pringle, K.G., Roberts, C.T., New light on early post-implantation pregnancy in the mouse: Roles for insulin-like growth factor-II (IGFII)? (2007) Placenta, 28, pp. 286-296
  • Rout, U.K., Armant, D.R., Expression of genes for alcohol and aldehyde metabolizing enzymes in mouse oocytes and preimplantation embryos (2002) Reprod Toxicol, 16, pp. 253-258
  • Sasaki, H., Mechanisms of trophectoderm fate specification in preimplantation mouse development (2010) Dev Growth Differ, 52, pp. 263-273
  • Shiina, Y., Kaneda, M., Matsuyama, K., Role of the extracellular Ca2 on the intracellular Ca2 changes in fertilized and activated mouse oocytes (1993) J Reprod Fertil, 97, pp. 143-150
  • Simmons, D.G., Fortier, A.L., Cross, J.C., Diverse subtypes and developmental origins of trophoblast giant cells in the mouse placenta (2007) Dev Biol, 304, pp. 567-578
  • Stachecki, J., Yelian, F.D., Leach, R.E., Mouse blastocyst outgrowth and implantation rates following exposure to ethanol or A23187 during culture in vitro (1994) J Reprod Fertil, 101, pp. 611-617
  • Stachecki, J.J., Yelian, F.D., Schultz, J.F., Blastocyst cavitation is accelerated by ethanol- or ionophore-induced elevation of intracellular calcium (1994) Biol Reprod, 50, pp. 1-9
  • Staun-Ram, E., Shalev, E., Human trophoblast function during the implantation process (2005) Reprod Biol Endocrinol, 3, pp. 56-68
  • Swann, A.C., Ethanol and (Na,K)-ATPase: Alteration of Na- K selectivity (1990) Alcohol Clin Exp Res, 14, pp. 922-927
  • Takase, K., Ishikawa, M., Hoshiai, H., Apoptosis on degeneration process of unfertilized mouse ova (1995) Tohoku J Exp Med, 175, pp. 69-76
  • Tarkowski, A.K., An air drying method for chromosome preparations from mouse eggs (1966) Cytogenetics (Basel), 5, pp. 394-400
  • Theiler, K., (1989) The House Mouse: Atlas of Embryonic Development, , New York: Springer-Verlag
  • Tournaye, H., Van Der Linden, M., Van Den Abbeel, E., Effect of pentoxifyline on implantation and post-implantation development of mouse embryos in vitro (1993) Human Reprod, 8, pp. 1948-1954
  • Yelian, F.D., Yang, Y., Hirata, J.D., Molecular interactions between fibronectin and integrins during mouse blastocyst outgrowth (1995) Mol Reprod Dev, 41, pp. 435-448

Citas:

---------- APA ----------
Pérez-Tito, L., Bevilacqua, E. & Cebral, E. (2014) . Peri-implantational in vivo and in vitro embryo-trophoblast development after perigestational alcohol exposure in the CD-1 mouse. Drug and Chemical Toxicology, 37(2), 184-197.
http://dx.doi.org/10.3109/01480545.2013.834358
---------- CHICAGO ----------
Pérez-Tito, L., Bevilacqua, E., Cebral, E. "Peri-implantational in vivo and in vitro embryo-trophoblast development after perigestational alcohol exposure in the CD-1 mouse" . Drug and Chemical Toxicology 37, no. 2 (2014) : 184-197.
http://dx.doi.org/10.3109/01480545.2013.834358
---------- MLA ----------
Pérez-Tito, L., Bevilacqua, E., Cebral, E. "Peri-implantational in vivo and in vitro embryo-trophoblast development after perigestational alcohol exposure in the CD-1 mouse" . Drug and Chemical Toxicology, vol. 37, no. 2, 2014, pp. 184-197.
http://dx.doi.org/10.3109/01480545.2013.834358
---------- VANCOUVER ----------
Pérez-Tito, L., Bevilacqua, E., Cebral, E. Peri-implantational in vivo and in vitro embryo-trophoblast development after perigestational alcohol exposure in the CD-1 mouse. Drug Chem. Toxicol. 2014;37(2):184-197.
http://dx.doi.org/10.3109/01480545.2013.834358