Artículo

La versión final de este artículo es de uso interno. El editor solo permite incluir en el repositorio el artículo en su versión post-print. Por favor, si usted la posee enviela a
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Chlorpyrifos is an organophosphate insecticide used for pest control on a number of food crops in many parts of the world. In recent years, there has been an important decrease in the number of organisms of Planorbarius corneus. Since the presence of pesticides in the water can be one of the reasons for this decrease, it is very important to study the effect of subchronic exposure to environmental concentrations of pesticides on these organisms. The aim of the present work was to investigate different effects of the subchronic exposure to low concentrations of the organophosphate chlorpyrifos in P. corneus and the possibility to use these as biomarkers. To this end, we have exposed the organisms to 0.4 and 5μgL-1 of chlorpyrifos for 14 days and recorded the number of egg masses, the number of eggs per mass, the number of eggs without embryo, the time for hatching, and the % of hatching and survival. We have also determined the activities of cholinesterases, carboxylesterases and glutathione S-transferase in whole organism soft tissue and in the gonads. A 14 days exposure to 0.4μgL-1 caused an increase in the number of egg masses without eggs and a decrease in carboxylesterases measured with p-nitrophenyl butyrate. However the exposure to 5μgL-1 also caused an increase in the time for hatching, a decrease in the % of hatching and survival and also inhibition of cholinesterases and carboxylesterases with p-nitrophenyl acetate and butyrate. In contrast, the glutathione S-transferase has not been modified with the tested concentrations. We concluded that when P. corneus exposed to chlorpyrifos for 14 days, the CES determined with p-nitrophenyl butyrate proved to be the most sensitive biomarker. However, exposure to environmental concentrations showed a decrease in the reproduction ability which could cause a decrease in the number of organisms of this species. © 2012 Elsevier Inc.

Registro:

Documento: Artículo
Título:Different effects of subchronic exposure to low concentrations of the organophosphate insecticide chlorpyrifos in a freshwater gastropod
Autor:Rivadeneira, P.R.; Agrelo, M.; Otero, S.; Kristoff, G.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Nuñez, 1428 Buenos Aires, Argentina
IQUIBICEN-CONICET, Ciudad Universitaria, Nuñez, 1428 Buenos Aires, Argentina
Palabras clave:Carboxylesterases; Chlorpyrifos; Cholinesterases; Glutathione S-transferase; Planorbarius corneus; Reproduction; carboxylesterase; chlorpyrifos; cholinesterase; fresh water; glutathione transferase; biomarker; chlorpyrifos; concentration (composition); gastropod; hatching; inhibition; insecticide; organophosphate; pesticide; survival; animal tissue; article; chronic toxicity; concentration response; controlled study; egg size; embryo; environmental exposure; enzyme activity; enzyme inhibition; gastropod; gonad; hatching; nonhuman; Planorbarius corneus; progeny; reproductive toxicity; soft tissue; survival rate; toxicity testing; water pollution indicator; Animals; Carboxylic Ester Hydrolases; Chlorpyrifos; Enzyme Activation; Enzymes; Fresh Water; Gastropoda; Gonads; Oviposition; Water Pollutants, Chemical
Año:2013
Volumen:90
Página de inicio:82
Página de fin:88
DOI: http://dx.doi.org/10.1016/j.ecoenv.2012.12.013
Título revista:Ecotoxicology and Environmental Safety
Título revista abreviado:Ecotoxicol. Environ. Saf.
ISSN:01476513
CODEN:EESAD
CAS:carboxylesterase, 59536-71-9, 83380-83-0, 9016-18-6, 9028-01-7; chlorpyrifos, 2921-88-2; cholinesterase, 9001-08-5; glutathione transferase, 50812-37-8; Carboxylic Ester Hydrolases, 3.1.1.-; Chlorpyrifos, 2921-88-2; Enzymes; Water Pollutants, Chemical
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01476513_v90_n_p82_Rivadeneira

Referencias:

  • Amanullah, B., Stalin, A., Prabu, P., Dhanapal, S., Analisis of AChE and LDH in mollusc, Lamellidens marginalis after exposure to chlorpirifos (2010) J. Environ. Biol., 31, pp. 417-419
  • Arufe, M.I., Arellano, J.M., García, L., Albendín, G., Sarasquete, C., Cholinesterase activity in gilthead seabream (Sparus aurata) larvae: characterization and sensitivity to the organophosphate azinphosmethyl (2007) Aquat. Toxicol., 84, pp. 328-336
  • Barata, C., Solayan, A., Porte, C., Role of B-esterases in assessing toxicity of organophosphorus (chlorpyrifos, malathion) and carbamate (carbofuran) pesticides to Daphia magna (2004) Aquat. Toxicol., 66, pp. 125-139
  • Barile, F.A., (2008) Principles of Toxicology Testing, , Taylor and Francis Group, CRC Press, USA
  • Barron, M.G., Woodburn, K.B., Ecotoxicology of chlorpyrifos (1995) Rev. Environ. Contam. Toxicol., 144, pp. 1-93
  • Basack, S.B., Oneto, M.L., Fuchs, J.S., Wood, E.J., Kesten, E.M., Esterases of Corbicula fluminea as biomarkers of exposure to organophosphorus pesticides (1998) Bull. Environ. Contam. Toxicol., 61, pp. 569-576
  • Bocquené, G., Roig, A., Fournier, D., Cholinesterases from the common oyster (Crassostrea gigas). Evidence for the presence of a soluble acetylcholinesterase insentive to organophosphate and carbamate inhibitors (1997) FEBS Lett., 407, pp. 261-266
  • Cacciatore, L.C., Kristoff, G., Verrengia Guerrero, N., Cochón, A., Binary mixtures of azinphos-methyl oxon and chlorpyrifos oxon produce in vitro synergistic cholinesterase inhibition in Planorbarius corneus (2012) Chemosphere, 88, pp. 450-458
  • Cacciatore, L.C., Villar, M.R., Kristoff, G., Verrengia Guerrero, N.R., Cochón, A., Inhibición in vivo de la actividad colinesterasa en tejidos blandos de un gastrópodo de agua dulce por el carbaril y mezclas binarias del mismo con metilazinfos o clorpirifos (2011) Acta Toxicol. Argent., (19), pp. 98-99
  • Clarke, N., Routledge, E.J., Garner, A., Casey, D., Benstead, R., Walker, D., Watermann, B., Jobling, S., Exposure to treated sewage effluent disrupts reproduction and development in the seasonally breeding ramshorn snail (Subclass: Pulmonata, Planorbarius corneus) (2009) Environ. Sci. Technol., 43, pp. 2092-2098
  • Collins Baker, F., (1945) The Molluscan Family Planorbidae, , The University of Illinois Press, Urbana, United States of America
  • Cooper, N.L., Bidwell, J.R., Cholinesterase inhibition and impacts on behavior of the Asian clam, Corbicula fluminea, after exposure to an organophosphate insecticide (2006) Aquat. Toxicol., 76, pp. 258-267
  • De Silva, P.M.C.S., Samayawardhena, L.A., Effects of chlorpyrifos on reproductive performances of guppy (Poecilia reticulata) (2005) Chemosphere, 58, pp. 1293-1299
  • Domingues, I., Guilhermino, L., Soares, A.M.V.M., Nogueira, A.J.A., Assessing dimethoate contamination in temperate and tropical climates: potencial use of biomarkers in bioassays with two chironomid species (2007) Chemosphere, (69), pp. 145-154
  • Edwards, C.A., Fisher, S.W., The use of cholinesterase measurements in assessing the impacts of pesticides on terrestrial and aquatic invertebrates (1991) Cholinesterase Inhibiting Insecticides: Their Impact on Wildlife and the Environment, pp. 255-275. , Elsevier, Amsterdam, Holand, P. Mineau (Ed.)
  • Ellman, G.L., Courtney, K.D., Andres, V., Featherstone, R.M., A new and rapid colorimetric determination of acetylcholinesterase activity (1961) Biochem. Pharmacol., 7, pp. 88-95
  • (2006), E.P.A. USEPA: Interim Registration Eligibility Decision for Chlorpyrifos; Escartin, E., Porte, C., Bioaccumulation, metabolism, and biochemical effects of the organphosphorus pesticide fenitrothion in Procambarus clarkii. Environ (1996) Toxicol. Chem., 15, pp. 915-920
  • Farag, A.T., Radwan, A.H., Sorour, F., El-Okazy, A., El-Agamy, E.S., El-Sebal, A.E.K., Chlorpyrifos induced reproductive toxicity in male mice (2010) Reprod. Toxicol., 29, pp. 80-85
  • Ferrari, A., Venturino, A., Pechen, D.'., Angelo, A.M., Time course of brain cholinesterase inhibition and recovery following acute and subacute azinphos methyl, parathion and carbaryl exposure in the goldfish (Carassius auratus). (2004) Ecotoxicol. Environ. Saf., 57, pp. 420-425
  • Ferrari, A., Venturino, A., Pechen, D.'., Angelo, A.M., Effects of carbaryl and azinphos methyl on juveniles rainbow trout (Oncorhynchus mykiss) detoxifying enzymes (2007) Pestic. Biochem. Phys., 88, pp. 134-142
  • Gagnaire, B., Geffard, O., Xuereb, B., Margoum, C., Garric, J., Cholinesterase activities as potential biomarkers: characterization in two freshwater snails, Potamopyrgus antipodarum (Mollusca, Hydrobiidae, Smith 1889) and Valvata piscinalis (Mollusca, Valvatidae, Muller 1774) (2008) Chemosphere, 71, pp. 553-560
  • Habig, W.H., Pabst, M.J., Kakoby, W.B., Glutathione S-transferases. The first enzymatic step in mercapturic acid formation (1974) J. Biol. Chem., 249, pp. 7130-7139
  • Hannam, M.L., Hagger, J.A., Jones, M.B., Galloway, T.S., Characterisation of esterases as potential biomarkers of pesticide exposure in the lugworm Arenicola marina (Annelida: Polychaeta) (2008) Environ. Pollut., 152, pp. 342-350
  • Hutchinson, T.H., Shillabeer, N., Winter, M.J., Pickford, D.B., Acute and chronic effects of carrier solvents in aquatic organisms: a critical review (2006) Aquat. Toxicology, 76, pp. 69-92
  • Jager, T., Crommentuijn, T., van Gestel, C.A.M., Kooijman, S.A.L.M., Chronic exposure to chlorpyrifos reveals two modes of action in the springtail Folsomia candida (2007) Environ. Pollut., 145, pp. 452-458
  • Jokanovic, M., Biotransformation of organophosphorus compounds (2001) Toxicology, 166, pp. 139-160
  • Jopp, F., Comparative studies on the dispersal of the Great Ramshorn (Planorbarius corneus L.): a modelling approach (2006) Limnologica, 36, pp. 17-25
  • Korochkin, L.I., Genetic regulation of isozyme patterns in Drosophila during development (1980) Isozymes: Current Tropics in Medical and Biological Research, 304, pp. 159-202. , Alan R. Liss, New York
  • Kristoff, G., Estudio comparativo de biomarcadores en los invertebrados acuáticos Biomphalaria glabrata y Lumbriculus variegatus expuestos a pesticidas de relevancia ambiental (2010), Tesis de Doctorado, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina; Kristoff, G., Cacciatore, L.C., Verrengia Guerrero, N.R., Cochón, A.C., Effects of the organophosphate insecticide azinphos-methyl on the reproduction and cholinesterase activity of Biomphalaria glabrata (2011) Chemosphere, 84, pp. 585-591
  • Kristoff, G., Chiny Barrionuevo, D., Cacciatore, L.C., Verrengia Guerrero, N.R., Cochón, A., In vivo studies on inhibition and recuperation of B-esterase activity in Biomphalaria glabrata exposed to azinphos-methyl: analysis of enzyme, substrate and tissue dependence (2012) Aquat. Toxicol., 112-113, pp. 19-26
  • Kristoff, G., Verrengia Guerrero, N., Cochón, A., Effects of azinphos-methyl exposure on enzymatic and non-enzymatic antioxidant defenses in Biomphalaria glabrata and Lumbriculus variegatus (2008) Chemosphere, 72, pp. 1333-1339
  • Kristoff, G., Verrengia Guerrero, N.R., Pechén de D'Angelo, A.M., Cochón, A.C., Inhibition of cholinesterase activity by azinphos-methyl in two freshwater invertebrates: Biomphalaria glabrata and Lumbriculus variegatus (2006) Toxicology, 222, pp. 185-194
  • Kubli, E., The sex peptide (1992) Bio Essays, 14, pp. 779-784
  • Kumar, A., Doan, H., Barnes, M., Chapman, J.C., Kookana, R.S., Response and recovery of acethylcholinesterase activity in freshwater shrimp Paratya australiensis (Decapoda: Atyidae) exposed to selected anti-cholinesterase insecticides (2010) Ecotoxicol. Environ. Saf, pp. 1503-1510
  • Lacorte, S., Lartiges, S.B., Garrigues, P., Barcelo, D., Degradation of organophosphorus pesticides and their transformation products in estuarine waters (1995) Environ. Sci. Technol., 29, pp. 431-438
  • Li-Xia, K., Yi-Long, X., Chun Wang, Z., Li-Li, D., Effects of three organophosphorus pesticides on population, growth and sexual reproduction of rotifer Brachionus calyciflorus Pallas (2009) Acta Entomol. Sinica., 29, pp. 182-185
  • Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J., Protein measurement with the folin phenol reagent (1951) J. Biol. Chem., 193, pp. 265-275
  • Mikhailov, A.I., Torrado, M., Korochkin, L.I., Kopantzeva, M.A., Méndez, J., Male predominant carboxylesterase expression in the reproductive system of molluscs and insects: immunochemical and biochemical similarity between Mytilus male associated polypeptide (MAP) and Drosophila sex-specific esterase S (1997) Comp. Biochem. Physiol., 118, pp. 197-208
  • Moore, M.T., Schulz, R., Cooper, C.M., Smith, S., Rodgers, J.H., Mitigation of chlorpyrifos run off using constructed wetlands (2002) Chemosphere, 46, pp. 827-835
  • Mora, P., Michel, X., Narbonne, J.-F., Cholinesterase activity as potencial biomarker in two bivalves (1999) Environ. Toxicol. Pharmacol., 7, pp. 253-260
  • Palma, P., Palma, V.L., Fernández, R.M., Bohn, A., Soares, A.M.V.M., Barbosa, I.R., Embryo-toxic effects of environmental concentrations of chlorpyrifos in the crustacean Daphnia magna (2009) Ecotoxicol. Environ. Saf, (72), pp. 1714-1718
  • Environmental fate of chlorpyrifos (1993) Rev. Environ. Contam. Toxicol., 131, pp. 1-150. , Racke
  • Rodríguez, J., Efecto del insecticida clorpirifos sobre las actividades de esterasas-B en Biomphalaria glabrata y Lumbriculus variegatus (2009) Tesis de licenciatura en Ciencias Biológicas, , Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
  • Roex, E.W.M., Keijzus, R., Van Gestel, C.A.M., Acetylcholinesterase inhibition and increased food consumption rale in zebrafish, Danio rerio after chronic exposure to parathion (2003) Aquat. Toxicol., 64, pp. 451-460
  • Sanchez-Hernandez, J.C., Ecotoxicological perspectives of B-esterases in the assessment of pesticide contamination (2007) Environmental Pollution: New Research, pp. 1-45. , Nova Science Publishers, Inc., N.Y., USA, R.H. Plattenberg (Ed.)
  • Strange, R.C., Jones, P.W., Fryer, A.A., Glutathione S-transferase: genetics and role in toxicology (2000) Toxicol. Lett., pp. 357-363
  • Strong, E.E., Gargominy, O., Ponder, W.F., Bouchet, P., Global diversity of gastropods (Gastropoda; Mollusca) in freshwater (2008) Hydrobiologia, 595, pp. 149-166
  • Talesa, V., Contenti, S., Mangiabene, C., Pascolini, R., Rosi, G., Principato, G.B., Propionylcholinesterase from Murex brandaris: comparison with other invertebrate cholinesterases (1990) Comp. Biochem. Physiol., 96, pp. 39-43
  • Timbrell, J.A., (2000) Principles of Biochemical Toxicology, , Taylor and Francis, London
  • Tripathi, P.K., Singh, A., Carbaryl induced alterations in the reproduction and metabolism of the freshwater snail Lymnaea acuminata (2004) Pest. Biochem. Physiol., 79, pp. 1-9
  • Van der Oost, R., Beyer, J., Vermeulen, N.P.E., Fish bioaccumulation and biomarkers in environmental risk assessment: a review (2003) Environ. Toxicol. Pharmacol., 13, pp. 57-149
  • Van Wijngaarden, R.P.A., Brock, T.C.M., Van den Brink, P.J., Threshold levels for effects of insecticides in freshwater ecosystems: a review (2005) Ecotoxicology, 14, pp. 355-380
  • Van Wijngaarden, R., Leeuwangh, P., Lucassen, W.G.H., Romijn, K., Ronday, R., Van der Velde, R., Willigenburg, W., Acute toxicity of chlorpyrifos to fish, a newt and aquatic invertebrates (1993) Bull. Environ. Contam. Toxicol., 51, pp. 716-723
  • Varó, I., Amat, F., Navarro, J.C., Barreda, M., Pitarch, E., Serrano, R., Assessment of the efficacy of Artemia sp. (Crustacea) cysts chorion as barrier to chlorpyrifos (organophosphorus pesticide) exposure. Effect on hatching and survival (2006) Sci. Total Environ., 366, pp. 148-153
  • Varó, I., Navarro, J.C., Amat, F., Guilhermino, L., Characterization of cholinesterases and evaluation of the inhibitory potential of chlorpyrifos and dichlorvos to Artemia salina and Artemia parthenogenetica (2002) Chemosphere, 48, pp. 563-569
  • Vioque-Fernández, A., Alves de Almeida, E., López-Barea, J., Esterases as pesticide biomarkers in crayfish (Procambarus clarkii, Crustacea): tissue distribution, sensitivity to model compounds and recovery from inactivation (2007) Comp. Biochem. Physiol., 145, pp. 404-412
  • Walker, C.H., Hopkin, S.P., Sibly, R.M., Peakall, D.B., (2001) Principles of Ecotoxicology, , Taylor and Francis, London
  • Organophosphorus insecticides: a General introduction (1986) Environmental Health Criteria, 63. , WHO, Geneva, WHO
  • Wiese, V., Nonmarine mollusca of Schleswig-Holstein (northernmost Germany) (2005), http://www.mollbase.org/sh/, (February 2005). MOLLBASE-National Database of German Land-and Freswater-Mollusks.01.06.2005; Wood, B., Starck, J.D., Acute toxicity of drainage ditch water from Washington State cranberry-growing region to Daphnia pulex in laboratory bioassays (2002) Ecotoxicol. Environ. Saf., 53, pp. 273-280
  • Xuereb, B., Noury, P., Felten, V., Garric, J., Geffard, O., Cholinesterase activity in Gammarus pulex (Crustacea Amphipoda): characterization and effects of chlorpyrifos (2007) Toxicology, 236, pp. 178-189
  • Zaliznick, L., Nugegoda, D., Effect of sublethal concentrations of chlorpyrifos on three successive generations of Daphnia carinata (2006) Ecotoxicol. Environ. Saf., 64, pp. 207-214

Citas:

---------- APA ----------
Rivadeneira, P.R., Agrelo, M., Otero, S. & Kristoff, G. (2013) . Different effects of subchronic exposure to low concentrations of the organophosphate insecticide chlorpyrifos in a freshwater gastropod. Ecotoxicology and Environmental Safety, 90, 82-88.
http://dx.doi.org/10.1016/j.ecoenv.2012.12.013
---------- CHICAGO ----------
Rivadeneira, P.R., Agrelo, M., Otero, S., Kristoff, G. "Different effects of subchronic exposure to low concentrations of the organophosphate insecticide chlorpyrifos in a freshwater gastropod" . Ecotoxicology and Environmental Safety 90 (2013) : 82-88.
http://dx.doi.org/10.1016/j.ecoenv.2012.12.013
---------- MLA ----------
Rivadeneira, P.R., Agrelo, M., Otero, S., Kristoff, G. "Different effects of subchronic exposure to low concentrations of the organophosphate insecticide chlorpyrifos in a freshwater gastropod" . Ecotoxicology and Environmental Safety, vol. 90, 2013, pp. 82-88.
http://dx.doi.org/10.1016/j.ecoenv.2012.12.013
---------- VANCOUVER ----------
Rivadeneira, P.R., Agrelo, M., Otero, S., Kristoff, G. Different effects of subchronic exposure to low concentrations of the organophosphate insecticide chlorpyrifos in a freshwater gastropod. Ecotoxicol. Environ. Saf. 2013;90:82-88.
http://dx.doi.org/10.1016/j.ecoenv.2012.12.013