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Abstract:

We studied the toxicity of a glyphosate formulation and provide evidence of metabolic alterations due to oxidative stress caused in a Chlorella kessleri tolerant strain by exposure to the herbicide. After 96h of exposure to increasing concentrations of the herbicide (0-70mgL-1) with alkylaryl polyglycol ether surfactant, growth was inhibited (EC50-96h 55.62mgL-1). Glyphosate increased protein and malondialdehyde content which was significantly higher than in the control at 50-70mgL-1. Superoxide dismutase and catalase activities and reduced glutathione levels increased in a concentration-dependant manner. Morphological studies showed increases in vacuolisation and in cell and sporangia sizes. The glyphosate formulation studied has a cytotoxic effect on C. kessleri through a mechanism that would involve the induction of oxidative stress. Upon glyphosate exposure, oxidative stress parameters such as SOD and CAT activities and MDA level could be more sensitive biomarkers than usually tested growth parameters in C. kessleri. © 2010 Elsevier Inc.

Registro:

Documento: Artículo
Título:Oxidative stress induced by a commercial glyphosate formulation in a tolerant strain of Chlorella kessleri
Autor:Romero, D.M.; Ríos de Molina, M.C.; Juárez, Á.B.
Filiación:Laboratorio de Enzimología, Estrés Oxidativo y Metabolismo, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas, Buenos Aires, Argentina
Departamento de Biodiversidad y Biología Experimental, Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Intendente Güiraldes 2160, C1428EGA Buenos Aires, Argentina
Palabras clave:Antioxidant defences; Chlorella kessleri; Glyphosate; Microalgae; Oxidative stress; algal protein; biological marker; catalase; glutathione; glyphosate; malonaldehyde; superoxide dismutase; surfactant; biomarker; concentration (composition); ecotoxicology; enzyme activity; free radical; glyphosate; green alga; inhibition; microalga; oxidation; pollution exposure; surfactant; algal growth; antioxidant activity; article; biomass; cell size; cell structure; cell vacuole; Chlorella; Chlorella kessleri; chlorophyll content; controlled study; cytotoxicity test; enzyme activity; growth inhibition; growth rate; herbicide tolerance; lipid peroxidation; nonhuman; oxidative stress; plant cell; plant metabolism; protein content; sensitivity analysis; sporangium; Biological Markers; Catalase; Chlorella; Glutathione; Glutathione Peroxidase; Glutathione Reductase; Glycine; Herbicides; Lipid Peroxidation; Malondialdehyde; Oxidative Stress; Superoxide Dismutase; Thiobarbituric Acid Reactive Substances; Water Pollutants, Chemical; Parachlorella kessleri
Año:2011
Volumen:74
Número:4
Página de inicio:741
Página de fin:747
DOI: http://dx.doi.org/10.1016/j.ecoenv.2010.10.034
Título revista:Ecotoxicology and Environmental Safety
Título revista abreviado:Ecotoxicol. Environ. Saf.
ISSN:01476513
CODEN:EESAD
CAS:catalase, 9001-05-2; glutathione, 70-18-8; glyphosate, 1071-83-6; malonaldehyde, 542-78-9; superoxide dismutase, 37294-21-6, 9016-01-7, 9054-89-1; Biological Markers; Catalase, 1.11.1.6; Glutathione, 70-18-8; Glutathione Peroxidase, 1.11.1.9; Glutathione Reductase, 1.8.1.7; Glycine, 56-40-6; Herbicides; Malondialdehyde, 542-78-9; Superoxide Dismutase, 1.15.1.1; Thiobarbituric Acid Reactive Substances; Water Pollutants, Chemical; glyphosate, 1071-83-6
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01476513_v74_n4_p741_Romero

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Citas:

---------- APA ----------
Romero, D.M., Ríos de Molina, M.C. & Juárez, Á.B. (2011) . Oxidative stress induced by a commercial glyphosate formulation in a tolerant strain of Chlorella kessleri. Ecotoxicology and Environmental Safety, 74(4), 741-747.
http://dx.doi.org/10.1016/j.ecoenv.2010.10.034
---------- CHICAGO ----------
Romero, D.M., Ríos de Molina, M.C., Juárez, Á.B. "Oxidative stress induced by a commercial glyphosate formulation in a tolerant strain of Chlorella kessleri" . Ecotoxicology and Environmental Safety 74, no. 4 (2011) : 741-747.
http://dx.doi.org/10.1016/j.ecoenv.2010.10.034
---------- MLA ----------
Romero, D.M., Ríos de Molina, M.C., Juárez, Á.B. "Oxidative stress induced by a commercial glyphosate formulation in a tolerant strain of Chlorella kessleri" . Ecotoxicology and Environmental Safety, vol. 74, no. 4, 2011, pp. 741-747.
http://dx.doi.org/10.1016/j.ecoenv.2010.10.034
---------- VANCOUVER ----------
Romero, D.M., Ríos de Molina, M.C., Juárez, Á.B. Oxidative stress induced by a commercial glyphosate formulation in a tolerant strain of Chlorella kessleri. Ecotoxicol. Environ. Saf. 2011;74(4):741-747.
http://dx.doi.org/10.1016/j.ecoenv.2010.10.034