Abstract:
The aim of this work was to assess the effects of 1 week copper exposure (6.2, 108, 210 and 414 μM) on Scenedesmus vacuolatus and Chlorella kessleri. The strains showed different susceptibility to copper. Copper content was determined in both strains by total X-ray reflection fluorescence analysis (TXRF). In S. vacuolatus, the increase of medium copper concentration induced an augmentation of protein and MDA content, and a significant decrease in the chlorophyll a/chlorophyll b ratio. S. vacuolatus showed a significant increase of catalase activity in 210 and 414 μM of copper, and a significant increment of SOD activity and GSH content only in 414 μM of copper. On the contrary, C. kessleri did not show significant differences in these parameters between 6.2 and 108 μM of copper. Increased copper in the environment evokes oxidative stress and an increase in the antioxidant defenses of S. vacuolatus. © 2009 Elsevier Inc. All rights reserved.
Registro:
Documento: |
Artículo
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Título: | Oxidative stress and antioxidant defenses in two green microalgae exposed to copper |
Autor: | Sabatini, S.E.; Juárez, A.B.; Eppis, M.R.; Bianchi, L.; Luquet, C.M.; Ríos de Molina, M.d.C. |
Filiación: | Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, 2 Pabellón, CP 1428, Buenos Aires, Argentina Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Av. Rivadavia, 1917 Buenos Aires, Argentina Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pab. II, Ciudad Universitaria, 1428 Buenos Aires, Argentina U.A. Combustibles Nucleares, C.A.C. Comisión Nacional de Energía Atómica, Av. Gral, Paz 1499, 1650, Pcia, Buenos Aires, Argentina Laboratorio de Ecotoxicología Acuática, INIBIOMA (CONICET-Universidad Nacional del Comahue) Pasaje de la Paz 235 San Martin de los Andes, 8370 Neuquén, Argentina
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Palabras clave: | Catalase; Chlorella kessleri; Copper content; Lipid peroxidation; Metabolic damage; Oxidative stress; Reduced glutathione; Scenedesmus vacuolatus; Superoxide dismutase; Total reflection X-ray fluorescence (TXRF); chlorophyll a; chlorophyll b; copper; antioxidant; chlorophyll; copper; enzyme activity; green alga; lipid; microalga; peptide; pollution effect; pollution exposure; protein; X-ray fluorescence; antioxidant responsive element; article; Chlorella; green alga; oxidative stress; Scenedesmus; water contamination; X ray fluorescence; Algal Proteins; Antioxidants; Catalase; Chelating Agents; Chlorella; Chlorophyll; Copper; Edetic Acid; Glutathione; Lipid Peroxidation; Malondialdehyde; Models, Statistical; Oxidation-Reduction; Oxidative Stress; Scenedesmus; Spectrometry, X-Ray Emission; Superoxide Dismutase; Parachlorella kessleri; Scenedesmus vacuolatus |
Año: | 2009
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Volumen: | 72
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Número: | 4
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Página de inicio: | 1200
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Página de fin: | 1206
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DOI: |
http://dx.doi.org/10.1016/j.ecoenv.2009.01.003 |
Título revista: | Ecotoxicology and Environmental Safety
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Título revista abreviado: | Ecotoxicol. Environ. Saf.
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ISSN: | 01476513
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CODEN: | EESAD
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CAS: | chlorophyll a, 479-61-8; chlorophyll b, 519-62-0; copper, 15158-11-9, 7440-50-8; Algal Proteins; Antioxidants; Catalase, 1.11.1.6; Chelating Agents; Chlorophyll, 1406-65-1; Copper, 7440-50-8; Edetic Acid, 60-00-4; Glutathione, 70-18-8; Malondialdehyde, 542-78-9; Superoxide Dismutase, 1.15.1.1
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01476513_v72_n4_p1200_Sabatini |
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Citas:
---------- APA ----------
Sabatini, S.E., Juárez, A.B., Eppis, M.R., Bianchi, L., Luquet, C.M. & Ríos de Molina, M.d.C.
(2009)
. Oxidative stress and antioxidant defenses in two green microalgae exposed to copper. Ecotoxicology and Environmental Safety, 72(4), 1200-1206.
http://dx.doi.org/10.1016/j.ecoenv.2009.01.003---------- CHICAGO ----------
Sabatini, S.E., Juárez, A.B., Eppis, M.R., Bianchi, L., Luquet, C.M., Ríos de Molina, M.d.C.
"Oxidative stress and antioxidant defenses in two green microalgae exposed to copper"
. Ecotoxicology and Environmental Safety 72, no. 4
(2009) : 1200-1206.
http://dx.doi.org/10.1016/j.ecoenv.2009.01.003---------- MLA ----------
Sabatini, S.E., Juárez, A.B., Eppis, M.R., Bianchi, L., Luquet, C.M., Ríos de Molina, M.d.C.
"Oxidative stress and antioxidant defenses in two green microalgae exposed to copper"
. Ecotoxicology and Environmental Safety, vol. 72, no. 4, 2009, pp. 1200-1206.
http://dx.doi.org/10.1016/j.ecoenv.2009.01.003---------- VANCOUVER ----------
Sabatini, S.E., Juárez, A.B., Eppis, M.R., Bianchi, L., Luquet, C.M., Ríos de Molina, M.d.C. Oxidative stress and antioxidant defenses in two green microalgae exposed to copper. Ecotoxicol. Environ. Saf. 2009;72(4):1200-1206.
http://dx.doi.org/10.1016/j.ecoenv.2009.01.003