Artículo

Iummato, M.M.; Fassiano, A.; Graziano, M.; dos Santos Afonso, M.; Ríos de Molina, M.D.C.; Juárez, Á.B."Effect of glyphosate on the growth, morphology, ultrastructure and metabolism of Scenedesmus vacuolatus" (2019) Ecotoxicology and Environmental Safety. 172:471-479
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Abstract:

The effects of a commercial glyphosate formulation on the oxidative stress parameters and morphology (including the ultrastructure) of the phytoplanktonic green microalga Scenedesmus vacuolatus were evaluated. After 96 h of exposure to increasing herbicide concentrations (0, 4, 6, 8 mg L −1 active ingredient) with the addition of alkyl aryl polyglycol ether surfactant, the growth of the cultures decreased (96 h-IC50- 4.90 mg L −1 ) and metabolic and morphology alterations were observed. Significant increases in cellular volume (103–353%) and dry weight (105%) and a significant decrease in pigment content (41–48%) were detected. Oxidative stress parameters were significantly affected, showing an increase in the reactive oxygen species (ROS) and reduced glutathione (GSH) contents, oxidative damage to lipids and proteins and a decrease in the activities of the antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT) and the detoxifying enzyme glutathione-S-transferase (GST). Cells exposed to glyphosate formulation were larger and showed an increase in vacuole size, bleaching, cell wall thickening and alteration of the stacking pattern of thylakoids. The results of this study showed the participation of oxidative stress in the mechanism of toxic action of the commercial glyphosate formulation on S. vacuolatus and the relation between the biochemical, morphological and ultrastructure alterations. © 2019 Elsevier Inc.

Registro:

Documento: Artículo
Título:Effect of glyphosate on the growth, morphology, ultrastructure and metabolism of Scenedesmus vacuolatus
Autor:Iummato, M.M.; Fassiano, A.; Graziano, M.; dos Santos Afonso, M.; Ríos de Molina, M.D.C.; Juárez, Á.B.
Filiación:Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Química Biológica, Buenos Aires, Argentina
CONICET‐ Universidad de Buenos Aires, Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN), Buenos Aires, Argentina
Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Química Inorgánica, CONICET-Universidad de Buenos Aires, Instituto de Química Física de los Materiales, Medio Ambiente y Energía (INQUIMAE), Buenos Aires, Argentina
Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Biodiversidad y Biología Experimental, CONICET-Universidad de Buenos Aires, Instituto de Biodiversidad y Biología Experimental y Aplicada (IBBEA), Buenos Aires, Argentina
Palabras clave:Biomarkers; Glyphosate; Microalgae; Oxidative stress; Scenedesmus vacuolatus; Ultrastructure; alkyl aryl polyglycol ether; catalase; glutathione; glutathione transferase; glyphosate; lipid; pigment; reactive oxygen metabolite; superoxide dismutase; surfactant; unclassified drug; biomarker; enzyme; glyphosate; green alga; growth rate; metabolism; microalga; morphology; oxidative stress; aquatic environment; Article; biochemistry; cell vacuole; cell volume; cell wall; concentration (parameter); controlled study; dry weight; environmental impact assessment; enzyme activity; growth rate; long term exposure; metabolism; morphology; nonhuman; oxidative stress; Scenedesmus; Scenedesmus vacuolatus; thylakoid; ultrastructure; Scenedesmus vacuolatus
Año:2019
Volumen:172
Página de inicio:471
Página de fin:479
DOI: http://dx.doi.org/10.1016/j.ecoenv.2019.01.083
Handle:http://hdl.handle.net/20.500.12110/paper_01476513_v172_n_p471_Iummato
Título revista:Ecotoxicology and Environmental Safety
Título revista abreviado:Ecotoxicol. Environ. Saf.
ISSN:01476513
CODEN:EESAD
CAS:catalase, 9001-05-2; glutathione, 70-18-8; glutathione transferase, 50812-37-8; glyphosate, 1071-83-6; lipid, 66455-18-3; superoxide dismutase, 37294-21-6, 9016-01-7, 9054-89-1
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01476513_v172_n_p471_Iummato

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Citas:

---------- APA ----------
Iummato, M.M., Fassiano, A., Graziano, M., dos Santos Afonso, M., Ríos de Molina, M.D.C. & Juárez, Á.B. (2019) . Effect of glyphosate on the growth, morphology, ultrastructure and metabolism of Scenedesmus vacuolatus. Ecotoxicology and Environmental Safety, 172, 471-479.
http://dx.doi.org/10.1016/j.ecoenv.2019.01.083
---------- CHICAGO ----------
Iummato, M.M., Fassiano, A., Graziano, M., dos Santos Afonso, M., Ríos de Molina, M.D.C., Juárez, Á.B. "Effect of glyphosate on the growth, morphology, ultrastructure and metabolism of Scenedesmus vacuolatus" . Ecotoxicology and Environmental Safety 172 (2019) : 471-479.
http://dx.doi.org/10.1016/j.ecoenv.2019.01.083
---------- MLA ----------
Iummato, M.M., Fassiano, A., Graziano, M., dos Santos Afonso, M., Ríos de Molina, M.D.C., Juárez, Á.B. "Effect of glyphosate on the growth, morphology, ultrastructure and metabolism of Scenedesmus vacuolatus" . Ecotoxicology and Environmental Safety, vol. 172, 2019, pp. 471-479.
http://dx.doi.org/10.1016/j.ecoenv.2019.01.083
---------- VANCOUVER ----------
Iummato, M.M., Fassiano, A., Graziano, M., dos Santos Afonso, M., Ríos de Molina, M.D.C., Juárez, Á.B. Effect of glyphosate on the growth, morphology, ultrastructure and metabolism of Scenedesmus vacuolatus. Ecotoxicol. Environ. Saf. 2019;172:471-479.
http://dx.doi.org/10.1016/j.ecoenv.2019.01.083