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Abstract:

Different concentrations of a glyphosate formulation, Roundup® Full II (66.2% glyphosate) were tested in culture peripheral blood of armadillo Chaetophractus villosus with cytogenetic biomarkers like mitotic index (MI), chromosomal aberrations (CA), sister chromatid exchange (SCE) and cell proliferation kinetics (CPK) by means of replication index. Adults animals of both sexes were exposed to RU at four concentrations ranging from 0.026 mL RU solution to 0.379 mL RU daily in oral treatment with the same volume (0.2 mL) during 7 days. We analyzed the induced damage at different times considering T0 as control value, one (T1), seven (T7) and 30 days (T30). One day after, only the higher concentration shows MI significant differences (p < 0.05), at T7 the frequency increases and at T30 it decreases reaching T0 values. The analysis of CA frequencies shows that only 0.106 mL RU/day exhibit significant differences vs T0 values. A great variability is expressed in the values of standard deviation (SD) and in the wide confidence intervals of the media. One day after treatments (T1) all four concentrations shows significant differences in SCE vs T0 values. Replication Index (RI) does not show significant differences. The dose-response behavior was not observed in either CA or SCE. The consistency of the findings obtained with the same biomarkers in vitro support the idea of expanding studies in order to characterize the risk doses for these mammals. © 2018 Elsevier Inc.

Registro:

Documento: Artículo
Título:Cytogenetic damage in peripheral blood cultures of Chaetophractus villosus exposed in vivo to a glyphosate formulation (Roundup)
Autor:Rossi, L.F.; Luaces, J.P.; Palermo, A.M.; Merani, M.S.; Mudry, M.D.
Filiación:Laboratorio de Biología Cromosómica, Facultad de Medicina, Universidad de Buenos Aires, C1121ABG Ciudad Autónoma de Buenos Aires, Argentina
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), C1425FQB Ciudad Autónoma de Buenos Aires, Argentina
Instituto de Investigaciones Científicas y Técnicas para la Defensa (CITEDEF), B1603ALQ Ciudad Autónoma de Buenos Aires, Argentina
Grupo de Investigación en Biología Evolutiva (GIBE), Depto EGE, IEGEBA, FCEyN, Universidad de Buenos Aires, C1428EGA Ciudad Autónoma de Buenos Aires, Argentina
Palabras clave:Chromosomal aberrations; Genotoxicity; Replication index; Roundup; Sister chromatid exchange; glyphosate; glycine; glyphosate; mutagenic agent; biomarker; blood; chromosome; cytogenetics; genotoxicity; glyphosate; mammal; adult; animal cell; animal experiment; armadillo; Article; cell proliferation; Chaetophractus villosus; chromosome aberration; chromosome breakage; concentration (parameters); controlled study; cytogenetics; female; genotoxicity; in vivo study; long term exposure; lymphocyte culture; male; mitosis index; nonhuman; sister chromatid exchange; analogs and derivatives; animal; blood; chemically induced; chromosome analysis; drug effect; human; lymphocyte; Animalia; Armadillo; Chaetophractus villosus; Mammalia; Animals; Armadillos; Cell Proliferation; Chromosome Aberrations; Cytogenetic Analysis; Female; Glycine; Humans; Lymphocytes; Male; Mitotic Index; Mutagens; Sister Chromatid Exchange
Año:2018
Volumen:157
Página de inicio:121
Página de fin:127
DOI: http://dx.doi.org/10.1016/j.ecoenv.2018.03.046
Título revista:Ecotoxicology and Environmental Safety
Título revista abreviado:Ecotoxicol. Environ. Saf.
ISSN:01476513
CODEN:EESAD
CAS:glyphosate, 1071-83-6; glycine, 56-40-6, 6000-43-7, 6000-44-8; Glycine; glyphosate; Mutagens
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01476513_v157_n_p121_Rossi

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Citas:

---------- APA ----------
Rossi, L.F., Luaces, J.P., Palermo, A.M., Merani, M.S. & Mudry, M.D. (2018) . Cytogenetic damage in peripheral blood cultures of Chaetophractus villosus exposed in vivo to a glyphosate formulation (Roundup). Ecotoxicology and Environmental Safety, 157, 121-127.
http://dx.doi.org/10.1016/j.ecoenv.2018.03.046
---------- CHICAGO ----------
Rossi, L.F., Luaces, J.P., Palermo, A.M., Merani, M.S., Mudry, M.D. "Cytogenetic damage in peripheral blood cultures of Chaetophractus villosus exposed in vivo to a glyphosate formulation (Roundup)" . Ecotoxicology and Environmental Safety 157 (2018) : 121-127.
http://dx.doi.org/10.1016/j.ecoenv.2018.03.046
---------- MLA ----------
Rossi, L.F., Luaces, J.P., Palermo, A.M., Merani, M.S., Mudry, M.D. "Cytogenetic damage in peripheral blood cultures of Chaetophractus villosus exposed in vivo to a glyphosate formulation (Roundup)" . Ecotoxicology and Environmental Safety, vol. 157, 2018, pp. 121-127.
http://dx.doi.org/10.1016/j.ecoenv.2018.03.046
---------- VANCOUVER ----------
Rossi, L.F., Luaces, J.P., Palermo, A.M., Merani, M.S., Mudry, M.D. Cytogenetic damage in peripheral blood cultures of Chaetophractus villosus exposed in vivo to a glyphosate formulation (Roundup). Ecotoxicol. Environ. Saf. 2018;157:121-127.
http://dx.doi.org/10.1016/j.ecoenv.2018.03.046