Artículo

Tolussi, C.E.; Gomes, A.D.O.; Kumar, A.; Ribeiro, C.S.; Nostro, F.L.L.; Bain, P.A.; de Souza, G.B.; Cuña, R.D.; Honji, R.M.; Moreira, R.G. "Environmental pollution affects molecular and biochemical responses during gonadal maturation of Astyanax fasciatus (Teleostei: Characiformes: Characidae)" (2018) Ecotoxicology and Environmental Safety. 147:926-934
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Abstract:

Endocrine disrupting compounds (EDCs) have the potential to alter fish reproduction at various levels of organization. The aim of this study was to assess the impact of a natural environment with heavily anthropogenic influence on the physiological processes involved in reproduction in the freshwater fish lambari (Astyanax fasciatus) using different biomarkers. Adult males and females were collected in different seasons from two distinct sites in the same watershed: Ponte Nova Reservoir (PN) considered a pristine or small anthropogenic influence reference point; and Billings Reservoir (Bil), subjected to a large anthropogenic impact. Biological indices, such as hepatosomatic index and gonadosomatic index (GSI), gonadal histomorphology, fecundity, and biomarkers such as plasma levels of estradiol (E2) as well as hepatic gene expression of its alfa nuclear receptor (ERα), were analyzed. Hepatic vitellogenin (VTG) gene expression was evaluated in both sexes, as an indicator of xenoestrogen exposure. Females collected at PN presented a typical annual variation reflected in GSI, whereas for those sampled at Bil the index did not change through the seasons. The higher concentration of E2 in males collected at Bil during spring/2013, together with the detection of VTG gene expression, suggest the presence of EDCs in the water. These EDCs may have also influenced fecundity of females from Bil, which was higher during winter and spring/2013. Gene expression of ERα and ovarian morphology did not differ between fish from both sites. Water conditions from Bil reservoir impacted by anthropic activity clearly interfered mainly with biomarkers of biological effect such as plasma E2 levels and absolute and relative fecundity, but also altered biomarkers of exposure as VTG gene expression. These facts support the notion that waterborne EDCs are capable of causing estrogenic activity in A. fasciatus. © 2017 Elsevier Inc.

Registro:

Documento: Artículo
Título:Environmental pollution affects molecular and biochemical responses during gonadal maturation of Astyanax fasciatus (Teleostei: Characiformes: Characidae)
Autor:Tolussi, C.E.; Gomes, A.D.O.; Kumar, A.; Ribeiro, C.S.; Nostro, F.L.L.; Bain, P.A.; de Souza, G.B.; Cuña, R.D.; Honji, R.M.; Moreira, R.G.
Filiación:Laboratório de Metabolismo e Reprodução de Organismos Aquáticos, Departamento de Fisiologia, Instituto de Biociências, Universidade de São Paulo. Rua do Matão, Trav.14, n° 321, São Paulo, SP 05508-090, Brazil
Environmental Contaminant Mitigation and Technologies Program, CSIRO Land and Water, Private Bag No. 2, Glen Osmond, SA 5064, Australia
Departamento de Biologia e Zootecnia, Universidade Estadual Paulista Júlio de Mesquita Filho, Campus de Ilha Solteira, Rua Monção, n°226, Ilha Solteira, 15385-000, Brazil
Laboratorio de Ecotoxicología Acuática, Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires & IBBEA, CONICET-UBA, Ciudad Universitaria (C1428EHA), Buenos Aires, Argentina
Centro de Aquicultura (CAUNESP). Universidade Estadual Paulista Júlio de Mesquita Filho, V. Acesso Prof. Paulo Donato Castelane s/n, Jaboticabal, SP 14884-900, Brazil
Palabras clave:Anthropogenic impact; Characidae; Endocrine disrupting compounds; Estradiol; Gene expression; Oocyte maturation; Vitellogenin; endocrine disruptor; estradiol; estrogen receptor alpha; vitellogenin; biological marker; endocrine disruptor; estradiol; fresh water; vitellogenin; anthropogenic effect; biochemistry; biomarker; endocrine disruptor; estrogenic compound; gene expression; maturation; molecular analysis; physiological response; pollution effect; teleost; adult; animal experiment; animal tissue; anthropometric parameters; Astyanax fasciatus; Brazil; Characidae; Characiformes; controlled study; estradiol blood level; estrogen activity; female; fertility; gene expression; gonad development; gonadosomatic index; hepatosomatic index; male; nonhuman; oocyte development; ovary development; pollution; Review; seasonal variation; teleost; watershed; analysis; animal; Characidae; chemistry; drug effects; environmental monitoring; gonad; growth, development and aging; metabolism; organogenesis; pathology; procedures; reproduction; season; toxicity; water pollutant; Astyanax fasciatus; Characidae; Characiformes; Teleostei; Animals; Biomarkers; Brazil; Characidae; Endocrine Disruptors; Environmental Monitoring; Estradiol; Female; Fertility; Fresh Water; Gene Expression; Gonads; Male; Organogenesis; Reproduction; Seasons; Vitellogenins; Water Pollutants, Chemical
Año:2018
Volumen:147
Página de inicio:926
Página de fin:934
DOI: http://dx.doi.org/10.1016/j.ecoenv.2017.09.056
Título revista:Ecotoxicology and Environmental Safety
Título revista abreviado:Ecotoxicol. Environ. Saf.
ISSN:01476513
CODEN:EESAD
CAS:estradiol, 50-28-2; Biomarkers; Endocrine Disruptors; Estradiol; Vitellogenins; Water Pollutants, Chemical
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01476513_v147_n_p926_Tolussi

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Citas:

---------- APA ----------
Tolussi, C.E., Gomes, A.D.O., Kumar, A., Ribeiro, C.S., Nostro, F.L.L., Bain, P.A., de Souza, G.B.,..., Moreira, R.G. (2018) . Environmental pollution affects molecular and biochemical responses during gonadal maturation of Astyanax fasciatus (Teleostei: Characiformes: Characidae). Ecotoxicology and Environmental Safety, 147, 926-934.
http://dx.doi.org/10.1016/j.ecoenv.2017.09.056
---------- CHICAGO ----------
Tolussi, C.E., Gomes, A.D.O., Kumar, A., Ribeiro, C.S., Nostro, F.L.L., Bain, P.A., et al. "Environmental pollution affects molecular and biochemical responses during gonadal maturation of Astyanax fasciatus (Teleostei: Characiformes: Characidae)" . Ecotoxicology and Environmental Safety 147 (2018) : 926-934.
http://dx.doi.org/10.1016/j.ecoenv.2017.09.056
---------- MLA ----------
Tolussi, C.E., Gomes, A.D.O., Kumar, A., Ribeiro, C.S., Nostro, F.L.L., Bain, P.A., et al. "Environmental pollution affects molecular and biochemical responses during gonadal maturation of Astyanax fasciatus (Teleostei: Characiformes: Characidae)" . Ecotoxicology and Environmental Safety, vol. 147, 2018, pp. 926-934.
http://dx.doi.org/10.1016/j.ecoenv.2017.09.056
---------- VANCOUVER ----------
Tolussi, C.E., Gomes, A.D.O., Kumar, A., Ribeiro, C.S., Nostro, F.L.L., Bain, P.A., et al. Environmental pollution affects molecular and biochemical responses during gonadal maturation of Astyanax fasciatus (Teleostei: Characiformes: Characidae). Ecotoxicol. Environ. Saf. 2018;147:926-934.
http://dx.doi.org/10.1016/j.ecoenv.2017.09.056