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Abstract:

We studied the accumulation and depuration of microcystin-LR (MCLR) in the hepatopancreas of the crab Neohelice granulata fed twice weekly with either non toxic or MCLR-producing Microcystis aeruginosa (strain NPDC1 or NPJB, respectively) during seven weeks. We also analyzed MCLR effects on the oxidative stress- and detoxification-related variables, superoxide dismutase and glutathione-S-transferase activities, and the levels of reduced glutathione and lipid peroxidation (as thiobarbituric acid reactive substances, TBARS).Hepatopancreas MCLR content slightly increased during the first three weeks, up to 8.81 ±1.84ng g-1 wet tissue mass (WTM) and then started to decrease to a minimum of 1.57 ±0.74ng g-1 WTM at the seventh week (p <0.05 with respect to that in the first week). TBARS levels were about 55% higher in treated than in control N. granulata (p <0.001 and p <0.05) during the first three weeks of the experimental period. GSH content became 50% lower than in control individuals (p <0.01) during weeks 6 and 7. SOD activity was increased by about 2-fold (p <0.05 or p<0.001) from week 3 to 7 in treated crabs with respect to control ones, while GST activity was about 70% higher in treated than in control crabs from week 4 to week 7 (p <0.05).Our data suggest that in the hepatopancreas of N. granulata MCLR accumulation and oxidative damage are limited and reversed by detoxification-excretion and antioxidant mechanisms. The activation of these defensive mechanisms becomes evident at 3-4 weeks after the start of the intoxication. © 2015 Elsevier Inc.

Registro:

Documento: Artículo
Título:Oxidative effects and toxin bioaccumulation after dietary microcystin intoxication in the hepatopancreas of the crab Neohelice (Chasmagnathus) granulata
Autor:Sabatini, S.E.; Brena, B.M.; Pirez, M.; de Molina, M.D.C.R.; Luquet, C.M.
Filiación:IQUIBICEN-Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, 2 Pabellón, 4 piso, Buenos Aires, CP 1428, Argentina
Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, 2 Pabellón, 4 pisoBuenos Aires, Argentina
Departamento de Biociencias, Cátedras de Bioquímica, Facultad de Química, Universidad de la República, Montevideo, Uruguay
LEA, INIBIOMA-CONICET-Universidad Nacional del Comahue, CEAN, Junín de los Andes, Argentina
Palabras clave:ELISA; Estuarine crab; Hepatopancreas; Microcystin depuration; Toxin bioaccumulation; glutathione; glutathione transferase; microcystin LR; superoxide dismutase; thiobarbituric acid reactive substance; antioxidant; enzyme inhibitor; glutathione; glutathione transferase; microcystin; superoxide dismutase; water pollutant; antioxidant; bioaccumulation; crab; detoxification; diet; ecotoxicology; estuarine ecosystem; excretion; phytotoxicity; toxin; adult; animal experiment; animal tissue; antioxidant activity; Article; bacterial strain; bioaccumulation; clinical trial (topic); controlled study; crab; detoxification; enzyme activity; excretion; hepatopancreas; host resistance; long term exposure; male; Microcystis aeruginosa; Neohelice granulata; nonhuman; oxidative stress; toxin analysis; xenobiotic metabolism; animal; Brachyura; drug effects; hepatopancreas; lipid peroxidation; metabolism; Microcystis; oxidative stress; pharmacokinetics; physiology; toxicity; water pollutant; Chasmagnathus; Decapoda (Crustacea); Microcystis aeruginosa; Animals; Antioxidants; Brachyura; Enzyme Inhibitors; Glutathione; Glutathione Transferase; Hepatopancreas; Lipid Peroxidation; Microcystins; Microcystis; Oxidative Stress; Superoxide Dismutase; Water Pollutants, Chemical
Año:2015
Volumen:120
Página de inicio:136
Página de fin:141
DOI: http://dx.doi.org/10.1016/j.ecoenv.2015.05.041
Título revista:Ecotoxicology and Environmental Safety
Título revista abreviado:Ecotoxicol. Environ. Saf.
ISSN:01476513
CODEN:EESAD
CAS:glutathione, 70-18-8; glutathione transferase, 50812-37-8; microcystin LR, 101043-37-2; superoxide dismutase, 37294-21-6, 9016-01-7, 9054-89-1; Antioxidants; Enzyme Inhibitors; Glutathione; Glutathione Transferase; microcystin; Microcystins; Superoxide Dismutase; Water Pollutants, Chemical
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01476513_v120_n_p136_Sabatini

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Citas:

---------- APA ----------
Sabatini, S.E., Brena, B.M., Pirez, M., de Molina, M.D.C.R. & Luquet, C.M. (2015) . Oxidative effects and toxin bioaccumulation after dietary microcystin intoxication in the hepatopancreas of the crab Neohelice (Chasmagnathus) granulata. Ecotoxicology and Environmental Safety, 120, 136-141.
http://dx.doi.org/10.1016/j.ecoenv.2015.05.041
---------- CHICAGO ----------
Sabatini, S.E., Brena, B.M., Pirez, M., de Molina, M.D.C.R., Luquet, C.M. "Oxidative effects and toxin bioaccumulation after dietary microcystin intoxication in the hepatopancreas of the crab Neohelice (Chasmagnathus) granulata" . Ecotoxicology and Environmental Safety 120 (2015) : 136-141.
http://dx.doi.org/10.1016/j.ecoenv.2015.05.041
---------- MLA ----------
Sabatini, S.E., Brena, B.M., Pirez, M., de Molina, M.D.C.R., Luquet, C.M. "Oxidative effects and toxin bioaccumulation after dietary microcystin intoxication in the hepatopancreas of the crab Neohelice (Chasmagnathus) granulata" . Ecotoxicology and Environmental Safety, vol. 120, 2015, pp. 136-141.
http://dx.doi.org/10.1016/j.ecoenv.2015.05.041
---------- VANCOUVER ----------
Sabatini, S.E., Brena, B.M., Pirez, M., de Molina, M.D.C.R., Luquet, C.M. Oxidative effects and toxin bioaccumulation after dietary microcystin intoxication in the hepatopancreas of the crab Neohelice (Chasmagnathus) granulata. Ecotoxicol. Environ. Saf. 2015;120:136-141.
http://dx.doi.org/10.1016/j.ecoenv.2015.05.041