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Abstract:

Endosulfan (ES) is an organochlorine pesticide widely used in agriculture despite its high toxicity towards non-target organisms such as fish. It has been demonstrated that ES can cause negative effects on aquatic animals, including disruption of hormonal systems. However, the alterations produced by this pesticide on the reproductive axis of fish prior to sexual maturity, as well as possible modes of action have hardly been studied. This study aimed at assessing the effect of waterborne exposure to the pesticide ES on the reproductive axis during sexual differentiation of juveniles of the South American freshwater cichlid fish Cichlasoma dimerus. No mortality was observed due to ES subchronic exposure (90 days post-fertilization). Exposure to ES did not affect body weight nor morphometric parameters, indicating that larvae nutritional state was not affected. Timing of sexual differentiation, gonadal morphology and sex ratio were likewise not altered by ES. However, ES acted as an endocrine disrupting chemical in this species as the morphometry of gonadotropin-releasing hormones (GnRH) producing cells was altered. Exposure to ES altered nuclear area, cell area and nucleus/cytoplasm ratio of GnRH II neurons, and cell and nuclear area and diameter of GnRH III neurons. Interestingly, in our previous study, exposure before sex differentiation (30 day exposure) caused no alteration to GnRH II and III, and did alter GnRH I and FSH cells. These alterations could lead to changes in circulating hormone levels, especially when fish are exposed for prolonged periods, ultimately impairing reproductive fitness. C. dimerus juveniles can be an interesting biological model to perform toxicological studies with the intent to assess early disruption endpoints in the reproductive axis during development. © 2015 Elsevier Inc.

Registro:

Documento: Artículo
Título:Endosulfan affects GnRH cells in sexually differentiated juveniles of the perciform Cichlasoma dimerus
Autor:Piazza, Y.; Pandolfi, M.; Da Cuña, R.; Genovese, G.; Lo Nostro, F.
Filiación:Laboratorio de Ecotoxicología Acuática, Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón II, 4, piso, C1428EHA, Argentina
IBBEA, CONICET-UBA, Ciudad Universitaria, Pabellón II, 4, piso, C1428EHA, Argentina
Laboratorio de Neuroendocrinología y Comportamiento, Departamento de Biodiversidad y Biología Experimental, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Pabellón II, 4, piso, C1428EHA, Argentina
Palabras clave:Cichlids; GnRH; Gonadotrops; Organochlorine pesticides; Reproductive axis; endocrine disruptor; endosulfan; gonadorelin; chlorinated hydrocarbon; endocrine disruptor; endosulfan; fresh water; gonadorelin; insecticide; progonadoliberin I; protein precursor; water pollutant; ecotoxicology; endocrine disruptor; endosulfan; hormone; juvenile; morphometry; perciform; pollution effect; pollution exposure; reproductive behavior; sexual maturity; adult; animal cell; animal experiment; animal tissue; Article; biological model; body weight; brain nerve cell; cell nucleus; cell structure; Cichlasoma dimerus; cichlid; controlled study; cytoplasm; development; exposure; female; gonad; juvenile animal; larva; male; morphometrics; nonhuman; nutritional status; Perciformes; reproductive fitness; reproductive toxicity; sex differentiation; sex ratio; analysis of variance; animal; cichlid; drug effects; metabolism; nerve cell; physiology; toxicity; water pollutant; Animalia; Cichlasoma; Cichlidae; Perciformes; Analysis of Variance; Animals; Cichlids; Endocrine Disruptors; Endosulfan; Fresh Water; Gonadotropin-Releasing Hormone; Gonads; Hydrocarbons, Chlorinated; Insecticides; Larva; Neurons; Protein Precursors; Sex Differentiation; Water Pollutants, Chemical
Año:2015
Volumen:116
Página de inicio:150
Página de fin:159
DOI: http://dx.doi.org/10.1016/j.ecoenv.2015.03.013
Título revista:Ecotoxicology and Environmental Safety
Título revista abreviado:Ecotoxicol. Environ. Saf.
ISSN:01476513
CODEN:EESAD
CAS:endosulfan, 115-29-7; gonadorelin, 33515-09-2, 9034-40-6; Endocrine Disruptors; Endosulfan; Gonadotropin-Releasing Hormone; Hydrocarbons, Chlorinated; Insecticides; progonadoliberin I; Protein Precursors; Water Pollutants, Chemical
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01476513_v116_n_p150_Piazza

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Citas:

---------- APA ----------
Piazza, Y., Pandolfi, M., Da Cuña, R., Genovese, G. & Lo Nostro, F. (2015) . Endosulfan affects GnRH cells in sexually differentiated juveniles of the perciform Cichlasoma dimerus. Ecotoxicology and Environmental Safety, 116, 150-159.
http://dx.doi.org/10.1016/j.ecoenv.2015.03.013
---------- CHICAGO ----------
Piazza, Y., Pandolfi, M., Da Cuña, R., Genovese, G., Lo Nostro, F. "Endosulfan affects GnRH cells in sexually differentiated juveniles of the perciform Cichlasoma dimerus" . Ecotoxicology and Environmental Safety 116 (2015) : 150-159.
http://dx.doi.org/10.1016/j.ecoenv.2015.03.013
---------- MLA ----------
Piazza, Y., Pandolfi, M., Da Cuña, R., Genovese, G., Lo Nostro, F. "Endosulfan affects GnRH cells in sexually differentiated juveniles of the perciform Cichlasoma dimerus" . Ecotoxicology and Environmental Safety, vol. 116, 2015, pp. 150-159.
http://dx.doi.org/10.1016/j.ecoenv.2015.03.013
---------- VANCOUVER ----------
Piazza, Y., Pandolfi, M., Da Cuña, R., Genovese, G., Lo Nostro, F. Endosulfan affects GnRH cells in sexually differentiated juveniles of the perciform Cichlasoma dimerus. Ecotoxicol. Environ. Saf. 2015;116:150-159.
http://dx.doi.org/10.1016/j.ecoenv.2015.03.013