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Abstract:

The effect of ampicillin (AMP), amoxicillin (AMX), cephalotin (CEP), ciprofloxacin (CPF), gentamycin (GEN), and vancomycin (VAN) have been examined individually and as binary mixtures, on a non-target aquatic organism, the green alga Pseudokichneriella subcapitata. The β-lactam antibiotics AMP and AMX were not toxic to the alga at concentrations up to 2000mgl-1 (less than 10% of algal growth inhibition), whereas the fluoroquinolone CPF, and the aminoglycoside GEN were the most toxic antibiotics, with an EC50=11.3±0.7mgl-1 and 19.2±0.5mgl-1, respectively. The cephalosporin CEP and the glycopeptide VAN were less toxic than the last two mentioned, showing an EC50>600mgl-1 and 724±20mgl-1, respectively. The toxicological interactions of binary mixtures were predicted by the two classical models of additivity: concentration addition (CA) and independent action (IA), and compared to the experimentally determined toxicities over a range of concentrations between 1 and 50mgl-1. In all cases a clear synergistic effect was observed, showing that single compound toxicity data are not adequate for the prediction of aquatic toxicities of antibiotic mixtures. Risk assessment was performed by calculating the ratio between predicted environmental concentrations (PEC) and the predicted no effect concentration (PNEC). All the antibiotics tested, excepting GEN, have a potential ecological risk, taking into account the PEC of hospital effluents from Buenos Aires, Argentina. These risks increase when antibiotics are present in binary mixtures. © 2014 Elsevier Inc.

Registro:

Documento: Artículo
Título:Effects of six antibiotics and their binary mixtures on growth of Pseudokirchneriella subcapitata
Autor:Magdaleno, A.; Saenz, M.E.; Juárez, A.B.; Moretton, J.
Filiación:Cátedra de Higiene y Sanidad, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Junin 956, 4 Piso, Buenos Aires, C1113AAC, Argentina
Dept. de Biodiversidad y Biología Experimental y Dept. de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Instituto de Biodiversidad y Biología Experimental y Aplicada (IBBEA-CONICET), Buenos Aires, Argentina
Palabras clave:Antibiotic toxicity; Green algae; Mixture toxicity; Risk quotient; Synergism; amoxicillin; ampicillin; cefalotin; ciprofloxacin; gentamicin; vancomycin; antiinfective agent; antibiotics; concentration (composition); ecotoxicology; green alga; risk assessment; synergism; toxicity; algal growth; Article; concentration (parameters); controlled study; ecotoxicity; effluent toxicity; environmental exposure; growth inhibition; molecular interaction; nonhuman; prediction; risk assessment; Selenastrum capricornutum; toxicity testing; waste water; water pollution; drug effects; drug interaction; green alga; growth, development and aging; Argentina; Buenos Aires [Argentina]; Chlorophyta; Selenastrum capricornutum; Anti-Bacterial Agents; Chlorophyta; Drug Interactions; Risk Assessment
Año:2015
Volumen:113
Página de inicio:72
Página de fin:78
DOI: http://dx.doi.org/10.1016/j.ecoenv.2014.11.021
Título revista:Ecotoxicology and Environmental Safety
Título revista abreviado:Ecotoxicol. Environ. Saf.
ISSN:01476513
CODEN:EESAD
CAS:amoxicillin, 26787-78-0, 34642-77-8, 61336-70-7; ampicillin, 69-52-3, 69-53-4, 7177-48-2, 74083-13-9, 94586-58-0; cefalotin, 153-61-7, 58-71-9; ciprofloxacin, 85721-33-1; gentamicin, 1392-48-9, 1403-66-3, 1405-41-0; vancomycin, 1404-90-6, 1404-93-9; Anti-Bacterial Agents
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01476513_v113_n_p72_Magdaleno

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Citas:

---------- APA ----------
Magdaleno, A., Saenz, M.E., Juárez, A.B. & Moretton, J. (2015) . Effects of six antibiotics and their binary mixtures on growth of Pseudokirchneriella subcapitata. Ecotoxicology and Environmental Safety, 113, 72-78.
http://dx.doi.org/10.1016/j.ecoenv.2014.11.021
---------- CHICAGO ----------
Magdaleno, A., Saenz, M.E., Juárez, A.B., Moretton, J. "Effects of six antibiotics and their binary mixtures on growth of Pseudokirchneriella subcapitata" . Ecotoxicology and Environmental Safety 113 (2015) : 72-78.
http://dx.doi.org/10.1016/j.ecoenv.2014.11.021
---------- MLA ----------
Magdaleno, A., Saenz, M.E., Juárez, A.B., Moretton, J. "Effects of six antibiotics and their binary mixtures on growth of Pseudokirchneriella subcapitata" . Ecotoxicology and Environmental Safety, vol. 113, 2015, pp. 72-78.
http://dx.doi.org/10.1016/j.ecoenv.2014.11.021
---------- VANCOUVER ----------
Magdaleno, A., Saenz, M.E., Juárez, A.B., Moretton, J. Effects of six antibiotics and their binary mixtures on growth of Pseudokirchneriella subcapitata. Ecotoxicol. Environ. Saf. 2015;113:72-78.
http://dx.doi.org/10.1016/j.ecoenv.2014.11.021