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Abstract:

The use of biomarkers as a tool to assess responses of organisms exposed to pollutants in toxicity bioassays, as well as in aquatic environmental risk assessment protocols, requires the understanding of the natural fluctuation of the particular biomarker. The aim of this study was to characterize the intrinsic variations of acetylcholinesterase (AChE) activity in tissues of a native freshwater teleost fish to be used as biomarker in toxicity tests, taking into account both seasonal influence and fish size.Specific AChE activity was measured by the method of Ellman et al. (1961) in homogenates of fish anterior section finding a seasonal variability. The highest activity was observed in summer, decreasing significantly below 40% in winter. The annual AChE activity cycle in the anterior section was fitted to a sinusoidal function with a period of 11.2 months. Moreover, an inverse relationship between enzymatic activity and the animal size was established. The results showed that both the fish length and seasonal variability affect AChE activity.AChE activity in fish posterior section showed a similar trend to that in the anterior section, while seasonal variations of the activity in midsection were observed but differences were not statistically significant.In addition, no relationship between AChE and total tissue protein was established in the anterior and posterior sections suggesting that the circannual rhythms observed are AChE-specific responses.Results highlight the importance of considering both the fish size and season variations to reach valid conclusions when AChE activity is employed as neurotoxicity biomarker. © 2014 Elsevier Inc.

Registro:

Documento: Artículo
Título:Circannual rhythms of acetylcholinesterase (AChE) activity in the freshwater fish Cnesterodon decemmaculatus
Autor:Menéndez-Helman, R.J.; Ferreyroa, G.V.; dos Santos Afonso, M.; Salibián, A.
Filiación:CONICET-INQUIMAE, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Pabellón 2, Ciudad Universitaria, Ciudad Autónoma de Buenos Aires, C1428EHA, Argentina
Programa de Ecofisiología Aplicada (PRODEA), Instituto de Ecología y Desarrollo Sustentable (INEDES), Universidad Nacional de Luján, Luján, B6700ZBA, Argentina
Palabras clave:Acetylcholinesterase; Biomarkers; Circannual rhythms; Cnesterodon decemmaculatus; Seasonal variations; acetylcholinesterase; acetylcholinesterase; biological marker; fresh water; water pollutant; bioassay; biomarker; environmental risk; freshwater environment; pollution exposure; protein; seasonal variation; teleost; toxicity; animal experiment; animal tissue; Article; biological monitoring; biological trait; circannual rhythm; Cnesterodon decemmaculatus; controlled study; ecotoxicology; environmental impact assessment; environmental stress; enzyme activity; enzyme mechanism; fish length; nonhuman; physical chemistry; physical parameters; risk assessment; seasonal variation; summer; teleost; toxicity testing; winter; animal; body size; Cyprinodontiformes; environmental monitoring; metabolism; photoperiodicity; procedures; season; temperature; toxicity; water pollutant; Cnesterodon decemmaculatus; Acetylcholinesterase; Animals; Biological Markers; Body Size; Cyprinodontiformes; Environmental Monitoring; Fresh Water; Photoperiod; Seasons; Temperature; Water Pollutants, Chemical
Año:2015
Volumen:111
Página de inicio:236
Página de fin:241
DOI: http://dx.doi.org/10.1016/j.ecoenv.2014.10.017
Título revista:Ecotoxicology and Environmental Safety
Título revista abreviado:Ecotoxicol. Environ. Saf.
ISSN:01476513
CODEN:EESAD
CAS:acetylcholinesterase, 9000-81-1; Acetylcholinesterase; Biological Markers; Water Pollutants, Chemical
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01476513_v111_n_p236_MenendezHelman

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Citas:

---------- APA ----------
Menéndez-Helman, R.J., Ferreyroa, G.V., dos Santos Afonso, M. & Salibián, A. (2015) . Circannual rhythms of acetylcholinesterase (AChE) activity in the freshwater fish Cnesterodon decemmaculatus. Ecotoxicology and Environmental Safety, 111, 236-241.
http://dx.doi.org/10.1016/j.ecoenv.2014.10.017
---------- CHICAGO ----------
Menéndez-Helman, R.J., Ferreyroa, G.V., dos Santos Afonso, M., Salibián, A. "Circannual rhythms of acetylcholinesterase (AChE) activity in the freshwater fish Cnesterodon decemmaculatus" . Ecotoxicology and Environmental Safety 111 (2015) : 236-241.
http://dx.doi.org/10.1016/j.ecoenv.2014.10.017
---------- MLA ----------
Menéndez-Helman, R.J., Ferreyroa, G.V., dos Santos Afonso, M., Salibián, A. "Circannual rhythms of acetylcholinesterase (AChE) activity in the freshwater fish Cnesterodon decemmaculatus" . Ecotoxicology and Environmental Safety, vol. 111, 2015, pp. 236-241.
http://dx.doi.org/10.1016/j.ecoenv.2014.10.017
---------- VANCOUVER ----------
Menéndez-Helman, R.J., Ferreyroa, G.V., dos Santos Afonso, M., Salibián, A. Circannual rhythms of acetylcholinesterase (AChE) activity in the freshwater fish Cnesterodon decemmaculatus. Ecotoxicol. Environ. Saf. 2015;111:236-241.
http://dx.doi.org/10.1016/j.ecoenv.2014.10.017