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Abstract:

Morphological and ultrastructural changes induced by exposure to excess of organic matter were analyzed in Phacus brachykentron (Pochm.). The cells were isolated from sites in Matanza River, Buenos Aires, Argentina, which have a high degree of organic matter contamination coming from waste waters discharges of the meat industry. Master strains were cultured on soil water medium and a toxicity bioassay was performed. As a result of the enriched medium, several morphological and ultrastructural cellular alterations were observed by optical, scanning, and transmission electron microscopy. Among these, we can point out changes in cell dimensions, remarkable widening of some pellicle bands, increased number and volume of paramylon grains, displacement of the nucleus from the central to the lateral position, some chloroplasts with their thylakoids disordered, and cell lysis. The response to organic enrichment was very fast, i.e. during the 48. h of the bioassay. Therefore, any significant increase of organic matter would rapidly affect wild euglenoids. Our results suggest that the alterations observed, such as the presence of large intracellular paramylon bodies or the deformation of euglenoid cells in natural samples, have the potential to be used as environmental bioindicators. © 2013 Elsevier Inc.

Registro:

Documento: Artículo
Título:Ultrastructural alterations in Phacus brachykentron (Euglenophyta) due to excess of organic matter in the culture medium
Autor:Nannavecchia, P.; Tolivia, A.; Conforti, V.
Filiación:Dpto. Biodiversidad y Biología Experimental, Facultad de Cs. Exactas y Naturales, UBA. 1428, Buenos Aires, Argentina
IBBEA, CONICET. Dpto. Biodiversidad y Biología Experimental, Facultad de Cs. Exactas y Naturales, UBA. 1428, Buenos Aires, Argentina
Palabras clave:Bioindicator; Euglenoids; Microalgae; Monitoring; Water quality; organic matter; bioassay; bioindicator; biomonitoring; concentration (composition); enrichment; microalga; morphology; organic matter; transmission electron microscopy; ultrastructure; wastewater; algal cell culture; algal growth; Argentina; article; cell nucleus; cell ultrastructure; chloroplast; controlled study; culture medium; cytolysis; Euglenida; growth rate; industrial waste; meat industry; nonhuman; Phacus brachykentron; transmission electron microscopy; water pollution; Argentina; Buenos Aires [Argentina]; Matanza-Riachuelo River; Bioindicator; Euglenoids; Microalgae; Monitoring; Water quality; Biological Assay; Environmental Monitoring; Euglenida; Microscopy, Electron, Scanning; Microscopy, Electron, Transmission; Water Pollutants, Chemical
Año:2014
Volumen:101
Número:1
Página de inicio:36
Página de fin:41
DOI: http://dx.doi.org/10.1016/j.ecoenv.2013.12.007
Título revista:Ecotoxicology and Environmental Safety
Título revista abreviado:Ecotoxicol. Environ. Saf.
ISSN:01476513
CODEN:EESAD
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01476513_v101_n1_p36_Nannavecchia

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Citas:

---------- APA ----------
Nannavecchia, P., Tolivia, A. & Conforti, V. (2014) . Ultrastructural alterations in Phacus brachykentron (Euglenophyta) due to excess of organic matter in the culture medium. Ecotoxicology and Environmental Safety, 101(1), 36-41.
http://dx.doi.org/10.1016/j.ecoenv.2013.12.007
---------- CHICAGO ----------
Nannavecchia, P., Tolivia, A., Conforti, V. "Ultrastructural alterations in Phacus brachykentron (Euglenophyta) due to excess of organic matter in the culture medium" . Ecotoxicology and Environmental Safety 101, no. 1 (2014) : 36-41.
http://dx.doi.org/10.1016/j.ecoenv.2013.12.007
---------- MLA ----------
Nannavecchia, P., Tolivia, A., Conforti, V. "Ultrastructural alterations in Phacus brachykentron (Euglenophyta) due to excess of organic matter in the culture medium" . Ecotoxicology and Environmental Safety, vol. 101, no. 1, 2014, pp. 36-41.
http://dx.doi.org/10.1016/j.ecoenv.2013.12.007
---------- VANCOUVER ----------
Nannavecchia, P., Tolivia, A., Conforti, V. Ultrastructural alterations in Phacus brachykentron (Euglenophyta) due to excess of organic matter in the culture medium. Ecotoxicol. Environ. Saf. 2014;101(1):36-41.
http://dx.doi.org/10.1016/j.ecoenv.2013.12.007