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Abstract:

Pseudomonas sp. 14-3 is an Antarctic bacterium that shows high stress resistance in association with high polyhydroxybutyrate (PHB) production. In this paper genes involved in PHB biosynthesis (phaRBAC) were found within a genomic island named pha-GI. Numerous mobile elements or proteins associated with them, such as an integrase, insertion sequences, a bacterial group II intron, a complete Type I protein secretion system and IncP plasmid-related proteins were detected among the 28 ORFs identified in this large genetic element (32.3 kb). The G + C distribution was not homogeneous, likely reflecting a mosaic structure that contains regions from diverse origins. pha-GI has strong similarities with genomic islands found in diverse Proteobacteria, including Burkholderiales species and Azotobacter vinelandii. The G + C content, phylogeny inference and codon usage analysis showed that the phaBAC cluster itself has a complex mosaic structure and indicated that the phaB and phaC genes were acquired by horizontal transfer, probably derived from Burkholderiales. These results describe for the first time a pha cluster located within a genomic island, and suggest that horizontal transfer of pha genes is a mechanism of adaptability to stress conditions such as those found in the extreme Antarctic environment. © 2007 Elsevier Inc. All rights reserved.

Registro:

Documento: Artículo
Título:The polyhydroxyalkanoate genes of a stress resistant Antarctic Pseudomonas are situated within a genomic island
Autor:Ayub, N.D.; Pettinari, M.J.; Méndez, B.S.; López, N.I.
Filiación:Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, 1428 Buenos Aires, Argentina
Palabras clave:Antarctica; Genomic islands; Polyhydroxyalkanoates; Pseudomonas; bacterial protein; polyhydroxyalkanoic acid; adaptation syndrome; Antarctica; article; Azotobacter vinelandii; bacterial gene; bacterial genome; bacterial strain; Burkholderia; codon usage; gene insertion sequence; gene sequence; genetic variability; horizontal gene transfer; intron; molecular phylogeny; nucleotide sequence; open reading frame; plasmid; protein secretion; Proteobacteria; sequence homology; unindexed sequence; Acyltransferases; Antarctic Regions; Bacterial Proteins; Base Composition; Base Sequence; Cloning, Molecular; DNA-Binding Proteins; Genes, Bacterial; Genomic Islands; Hydroxybutyrates; Molecular Sequence Data; Open Reading Frames; Phylogeny; Polyhydroxyalkanoates; Pseudomonas; Repressor Proteins; Sequence Alignment; Azotobacter vinelandii; Bacteria (microorganisms); Burkholderiales; Proteobacteria; Pseudomonas; Pseudomonas sp. 14-3
Año:2007
Volumen:58
Número:3
Página de inicio:240
Página de fin:248
DOI: http://dx.doi.org/10.1016/j.plasmid.2007.05.003
Título revista:Plasmid
Título revista abreviado:Plasmid
ISSN:0147619X
CODEN:PLSMD
CAS:Acyltransferases, EC 2.3.-; Bacterial Proteins; DNA-Binding Proteins; Hydroxybutyrates; PhaR protein, bacteria; poly(3-hydroxyalkanoic acid) synthase, EC 2.3.1.-; Polyhydroxyalkanoates; Repressor Proteins
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0147619X_v58_n3_p240_Ayub

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Citas:

---------- APA ----------
Ayub, N.D., Pettinari, M.J., Méndez, B.S. & López, N.I. (2007) . The polyhydroxyalkanoate genes of a stress resistant Antarctic Pseudomonas are situated within a genomic island. Plasmid, 58(3), 240-248.
http://dx.doi.org/10.1016/j.plasmid.2007.05.003
---------- CHICAGO ----------
Ayub, N.D., Pettinari, M.J., Méndez, B.S., López, N.I. "The polyhydroxyalkanoate genes of a stress resistant Antarctic Pseudomonas are situated within a genomic island" . Plasmid 58, no. 3 (2007) : 240-248.
http://dx.doi.org/10.1016/j.plasmid.2007.05.003
---------- MLA ----------
Ayub, N.D., Pettinari, M.J., Méndez, B.S., López, N.I. "The polyhydroxyalkanoate genes of a stress resistant Antarctic Pseudomonas are situated within a genomic island" . Plasmid, vol. 58, no. 3, 2007, pp. 240-248.
http://dx.doi.org/10.1016/j.plasmid.2007.05.003
---------- VANCOUVER ----------
Ayub, N.D., Pettinari, M.J., Méndez, B.S., López, N.I. The polyhydroxyalkanoate genes of a stress resistant Antarctic Pseudomonas are situated within a genomic island. Plasmid. 2007;58(3):240-248.
http://dx.doi.org/10.1016/j.plasmid.2007.05.003