Abstract:
Pseudomonas sp. 14-3 is an Antarctic bacterium that shows high stress resistance in association with high polyhydroxybutyrate (PHB) production. In this paper genes involved in PHB biosynthesis (phaRBAC) were found within a genomic island named pha-GI. Numerous mobile elements or proteins associated with them, such as an integrase, insertion sequences, a bacterial group II intron, a complete Type I protein secretion system and IncP plasmid-related proteins were detected among the 28 ORFs identified in this large genetic element (32.3 kb). The G + C distribution was not homogeneous, likely reflecting a mosaic structure that contains regions from diverse origins. pha-GI has strong similarities with genomic islands found in diverse Proteobacteria, including Burkholderiales species and Azotobacter vinelandii. The G + C content, phylogeny inference and codon usage analysis showed that the phaBAC cluster itself has a complex mosaic structure and indicated that the phaB and phaC genes were acquired by horizontal transfer, probably derived from Burkholderiales. These results describe for the first time a pha cluster located within a genomic island, and suggest that horizontal transfer of pha genes is a mechanism of adaptability to stress conditions such as those found in the extreme Antarctic environment. © 2007 Elsevier Inc. All rights reserved.
Registro:
Documento: |
Artículo
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Título: | The polyhydroxyalkanoate genes of a stress resistant Antarctic Pseudomonas are situated within a genomic island |
Autor: | Ayub, N.D.; Pettinari, M.J.; Méndez, B.S.; López, N.I. |
Filiación: | Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, 1428 Buenos Aires, Argentina
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Palabras clave: | Antarctica; Genomic islands; Polyhydroxyalkanoates; Pseudomonas; bacterial protein; polyhydroxyalkanoic acid; adaptation syndrome; Antarctica; article; Azotobacter vinelandii; bacterial gene; bacterial genome; bacterial strain; Burkholderia; codon usage; gene insertion sequence; gene sequence; genetic variability; horizontal gene transfer; intron; molecular phylogeny; nucleotide sequence; open reading frame; plasmid; protein secretion; Proteobacteria; sequence homology; unindexed sequence; Acyltransferases; Antarctic Regions; Bacterial Proteins; Base Composition; Base Sequence; Cloning, Molecular; DNA-Binding Proteins; Genes, Bacterial; Genomic Islands; Hydroxybutyrates; Molecular Sequence Data; Open Reading Frames; Phylogeny; Polyhydroxyalkanoates; Pseudomonas; Repressor Proteins; Sequence Alignment; Azotobacter vinelandii; Bacteria (microorganisms); Burkholderiales; Proteobacteria; Pseudomonas; Pseudomonas sp. 14-3 |
Año: | 2007
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Volumen: | 58
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Número: | 3
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Página de inicio: | 240
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Página de fin: | 248
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DOI: |
http://dx.doi.org/10.1016/j.plasmid.2007.05.003 |
Título revista: | Plasmid
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Título revista abreviado: | Plasmid
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ISSN: | 0147619X
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CODEN: | PLSMD
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CAS: | Acyltransferases, EC 2.3.-; Bacterial Proteins; DNA-Binding Proteins; Hydroxybutyrates; PhaR protein, bacteria; poly(3-hydroxyalkanoic acid) synthase, EC 2.3.1.-; Polyhydroxyalkanoates; Repressor Proteins
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0147619X_v58_n3_p240_Ayub |
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Citas:
---------- APA ----------
Ayub, N.D., Pettinari, M.J., Méndez, B.S. & López, N.I.
(2007)
. The polyhydroxyalkanoate genes of a stress resistant Antarctic Pseudomonas are situated within a genomic island. Plasmid, 58(3), 240-248.
http://dx.doi.org/10.1016/j.plasmid.2007.05.003---------- CHICAGO ----------
Ayub, N.D., Pettinari, M.J., Méndez, B.S., López, N.I.
"The polyhydroxyalkanoate genes of a stress resistant Antarctic Pseudomonas are situated within a genomic island"
. Plasmid 58, no. 3
(2007) : 240-248.
http://dx.doi.org/10.1016/j.plasmid.2007.05.003---------- MLA ----------
Ayub, N.D., Pettinari, M.J., Méndez, B.S., López, N.I.
"The polyhydroxyalkanoate genes of a stress resistant Antarctic Pseudomonas are situated within a genomic island"
. Plasmid, vol. 58, no. 3, 2007, pp. 240-248.
http://dx.doi.org/10.1016/j.plasmid.2007.05.003---------- VANCOUVER ----------
Ayub, N.D., Pettinari, M.J., Méndez, B.S., López, N.I. The polyhydroxyalkanoate genes of a stress resistant Antarctic Pseudomonas are situated within a genomic island. Plasmid. 2007;58(3):240-248.
http://dx.doi.org/10.1016/j.plasmid.2007.05.003