Abstract:
A polysaccharide with the properties of glycogen has been isolated by water or phenol-water extraction from homogenates of Ascobolus furfuraceus. After 4 days of growth a maximum glycogen content of 28% of the cell dry weight was found by a specific enzymic method or by the iodine method. Taking into account the cell growth, the highest total amount of glycogen (2.04 mg/ml of broth) occurred on the 10th day. The average chain length determined by periodate oxidation was about 11. This result shows that the reserve polysaccharide from A. furfuraceus has a highly branched structure similar to liver glycogen. © 1981.
Registro:
Documento: |
Artículo
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Título: | Isolation and characterization of glycogen from mycelium of Ascobolus furfuraceus |
Autor: | Groisman, J.F.; Forchiassin, F.; Ranalli, M.E.; de Lederkremer, R.M. |
Filiación: | Departmento de Química Orgánica, Facultad de Ciencias Exactas, Naturales Universidad de Buenos Aires, Ciudad Universitaria, Pabellon II, 1428 Buenos Aires, Argentina Departmento de Ciencias Biológicas, Facultad de Ciencias Exactas, Naturales Universidad de Buenos Aires, Ciudad Universitaria, Pabellon II, 1428 Buenos Aires, Argentina
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Palabras clave: | Ascobolus furfuraceus; coprophilous fungus; glycogen characteristics |
Año: | 1981
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Volumen: | 5
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Número: | 3
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Página de inicio: | 253
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Página de fin: | 257
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DOI: |
http://dx.doi.org/10.1016/0147-5975(81)90029-3 |
Título revista: | Experimental Mycology
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Título revista abreviado: | Exp. Mycol.
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ISSN: | 01475975
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CODEN: | EXMYD
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01475975_v5_n3_p253_Groisman |
Referencias:
- Archibald, Fleming, Liddle, Manners, Mercer, Wright, α-14-Glucosans Part XI The absorption spectra of glycogen and amylopectin iodine complexes (1961) Journal of the Chemical Society (Resumed), pp. 1183-1190
- Bueding, Orrell, Jr., Sedimentation coefficient distributions of cold water-extracted glycogens ofFasciola hepatica (1961) J. Biol. Chem., 236, pp. 2854-2856
- Dubois, Gilles, Hamilton, Rebers, Smith, Colorimetric method for determination of sugars and related substances (1956) Anal. Chem., 28, pp. 350-356
- Ergle, The glycogen content ofPhymatotrichum sclerotia (1947) J. Amer. Chem. Soc., 69, pp. 2061-2062
- Fleming, Pegler, The determination of glucose in the presence of maltose and isomaltose by a stable, specific enzymic reagent (1963) Analyst, 88, pp. 967-968
- Gamundi, Ranalli, Estudio sistema´tico y biologico de las Ascobola´ceas de la Argentina, II (1966) Nova Hedwigia, 10, pp. 339-366. , (la´minas 106, 1) 112 (7))
- Gorin, Spencer, Structural chemistry of fungal polysaccharides (1968) Advan. Carbohydr. Chem., 23, pp. 367-417
- Hough, Jones, Wadman, Quantitative analysis of mixture of sugars by the method of partition chromatography Part V Improved methods for separation and detection of the sugars and their methylated derivatives on the paper chromatogram (1950) Journal of the Chemical Society (Resumed), pp. 1702-1706
- Huijing, A rapid enzymic method for glycogen estimation in very small tissue samples (1970) Clin. Chim. Acta, 30, pp. 567-572
- Krisman, A method for the colorimetric estimation of glycogen with iodine (1962) Anal. Biochem., 4, pp. 17-23
- Laskov, Margoliash, Properties of high molecular wieght glycogen from rat liver (1963) Bull. Res. Counc. Isr. Sect. A Chem., 11 (4), pp. 351-362
- Northcote, A molecular structure and shape of yeast glycogen (1953) Biochem. J., 53, pp. 348-352
- Ranalli, Cinto, Estudio sistema´tico y biolo´gico de las Ascobola´ceas de Argentina. IV (1972) Bol. Soc. Argentina Bot., 14, pp. 285-304
- Ranalli, Forchiassin, Estudio sistema´tico y biolo´gico de las Ascobola´ceas de Argentina. VII (1976) Bol. Soc. Argentina Bot., 17, pp. 262-279
- Ranalli, Forchiassin, Estudio sistema´tico y biolo´gico de las Ascobola´ceas de Argentina. VIII.Ascobolus ferrugineus sp. nov. Estudios de cultivo y citologi´a (1978) Physis (Sect. C), 38, pp. 43-55
- Takahara, Matsuda, The structure ofNeurospora crassa glycogen (1976) Agr. Biol. Chem., 40, pp. 1699-1703
- Toennies, Kole, Techniques and reagents for paper chromatography (1951) Anal. Chem., 23, pp. 823-826
- Trevelyan, Procter, Harrison, Detection of sugars on paper chromatograms (1950) Nature (London), 166, pp. 444-445
Citas:
---------- APA ----------
Groisman, J.F., Forchiassin, F., Ranalli, M.E. & de Lederkremer, R.M.
(1981)
. Isolation and characterization of glycogen from mycelium of Ascobolus furfuraceus. Experimental Mycology, 5(3), 253-257.
http://dx.doi.org/10.1016/0147-5975(81)90029-3---------- CHICAGO ----------
Groisman, J.F., Forchiassin, F., Ranalli, M.E., de Lederkremer, R.M.
"Isolation and characterization of glycogen from mycelium of Ascobolus furfuraceus"
. Experimental Mycology 5, no. 3
(1981) : 253-257.
http://dx.doi.org/10.1016/0147-5975(81)90029-3---------- MLA ----------
Groisman, J.F., Forchiassin, F., Ranalli, M.E., de Lederkremer, R.M.
"Isolation and characterization of glycogen from mycelium of Ascobolus furfuraceus"
. Experimental Mycology, vol. 5, no. 3, 1981, pp. 253-257.
http://dx.doi.org/10.1016/0147-5975(81)90029-3---------- VANCOUVER ----------
Groisman, J.F., Forchiassin, F., Ranalli, M.E., de Lederkremer, R.M. Isolation and characterization of glycogen from mycelium of Ascobolus furfuraceus. Exp. Mycol. 1981;5(3):253-257.
http://dx.doi.org/10.1016/0147-5975(81)90029-3