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Abstract:

A major aminopeptidase was purified to apparent homogeneity from soluble extracts of the fungusSaccobolus platensis by DEAE-cellulose, phenyl-Sepharose, Sephacryl S-300 chromatography, and disc polyacrylamide gel electrophoresis. Peptidase activity was measured with the radioactive peptide substrate Leu-Arg-Arg-Ala-Ser[32P]-Leu-Gly. The purified enzyme was a monomeric protein with a molecular mass of 90 kDa. The optimun pH of the enzyme activity was in the range 7.4-7.6. It was inhibited remarkably by EDTA, 1,10-phenanthroline, puromycin, amastatin, bestatin, and hydrophobic amino acids. The enzyme had a low substrate specificity and a requirement for free α-amino groups. Saccobolus peptidase had no measurable endoproteolytic activity and the exoproteolytic activity was evidenced on small peptides, suggesting that it might be involved in the late stage of protein turnover events. © 1992 Academic Press, Inc.

Registro:

Documento: Artículo
Título:Purification and characterization of an aminopeptidase from the fungusSaccobolus platensis
Autor:Murray, P.F.; Samela, A.; Passeron, S.
Filiación:Departamento de Quimica Biolo ́gica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, 4to. piso, Pabello n II, 1428 Buenos Aires, Argentina
Palabras clave:ascomycetes; peptidase; peptide processing; phosphopeptide
Año:1992
Volumen:16
Número:4
Página de inicio:279
Página de fin:290
DOI: http://dx.doi.org/10.1016/0147-5975(92)90004-B
Título revista:Experimental Mycology
Título revista abreviado:Exp. Mycol.
ISSN:01475975
CODEN:EXMYD
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01475975_v16_n4_p279_Murray

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Citas:

---------- APA ----------
Murray, P.F., Samela, A. & Passeron, S. (1992) . Purification and characterization of an aminopeptidase from the fungusSaccobolus platensis. Experimental Mycology, 16(4), 279-290.
http://dx.doi.org/10.1016/0147-5975(92)90004-B
---------- CHICAGO ----------
Murray, P.F., Samela, A., Passeron, S. "Purification and characterization of an aminopeptidase from the fungusSaccobolus platensis" . Experimental Mycology 16, no. 4 (1992) : 279-290.
http://dx.doi.org/10.1016/0147-5975(92)90004-B
---------- MLA ----------
Murray, P.F., Samela, A., Passeron, S. "Purification and characterization of an aminopeptidase from the fungusSaccobolus platensis" . Experimental Mycology, vol. 16, no. 4, 1992, pp. 279-290.
http://dx.doi.org/10.1016/0147-5975(92)90004-B
---------- VANCOUVER ----------
Murray, P.F., Samela, A., Passeron, S. Purification and characterization of an aminopeptidase from the fungusSaccobolus platensis. Exp. Mycol. 1992;16(4):279-290.
http://dx.doi.org/10.1016/0147-5975(92)90004-B