Navegar

Colección

Datos Estadísticas

Artículo

La versión final de este artículo es de uso interno de la institución.
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

In Neurospora crassa grown in minimal medium with 2 μM cycloheximide, the expression of several genes was shown to be turned on. We have isolated one of those genes coding for a major transcript in this response, which newly appears after addition of cycloheximide and remains present at a high level for a long period. An open coding region for a 70-kDa protein was demonstrated by hybrid-selected translation, in agreement with the in vivo labeling of a protein with the same mobility in cycloheximide-induced cultures. Even 48 h after cycloheximide addition, CI-1-specific mRNA was bound to polysomal fractions. Our results also suggest that DNA sequences analogous to N. crassa CI-1 gene are present in other species. In N. crassa, CI-1 gene would not be induced by heat shock or starvation. © 1989.

Registro:

Documento: Artículo
Título:A major transcript in the response of Neurospora crassa to protein synthesis inhibition by cycloheximide
Autor:Aisemberg, G.O.; Grotewold, E.; Taccioli, G.E.; Judewicz, N.D.
Filiación:Instituto de Investigaciones en Ingenieria Genetica y Biologia Molecular INGEBI (CONICET), U.B.A. Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina
Facultad de Ciencias Exactas y Naturales, U.B.A. Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina
Palabras clave:cycloheximide; gene expression; Neurospora crassa; stress proteins
Año:1989
Volumen:13
Número:2
Página de inicio:121
Página de fin:128
DOI: http://dx.doi.org/10.1016/0147-5975(89)90017-0
Título revista:Experimental Mycology
Título revista abreviado:Exp. Mycol.
ISSN:01475975
CODEN:EXMYD
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01475975_v13_n2_p121_Aisemberg

Referencias:

  • Aisemberg, Taccioli, Grotewold, Judewicz, Differential gene expression promoted by cycloheximide inNeurospora crassa (1987) Exp. Mycol., 11, pp. 122-127
  • Aviv, Leder, Purification of biologically active globin messenger RNA by chromatography on oligo-thymidylic acid-cellulose (1972) Proc. Natl. Acad. Sci. USA, 69, pp. 1408-1412
  • Chamberlain, Fluorographic detection of radioactivity in polyacrylamide gels with water-soluble fluors: Sodium salicylate (1979) Anal. Biochem., 98, pp. 132-135
  • Fro¨ehner, Eriksson, Induction ofNeurospora crassa laccase with protein synthesis inhibitors (1974) J. Bacteriol., 120, pp. 450-457
  • Horowitz, Evidence for common control of tyrosinase andl-amino-acid oxidase inNeurospora crassa (1965) Biochem. Biophys. Res. Commun., 18, pp. 686-692
  • Horowitz, Feldman, Pall, Derepression of tyrosinase synthesis inNeurospora by cycloheximide actinomycin D and puromycin (1970) J. Biol. Chem., 245, pp. 2784-2788
  • Horowitz, Fling, Macleod, Watanabe, Structural and regulative genes controlling tyrosinase synthesis inNeurospora (1961) Cold Spring Harbor Symp. Quant. Biol., 26, pp. 233-238
  • Kapoor, Lewis, Alteration of the protein synthesis pattern inNeurospora crassa cells by hyperthermal and oxidative stress (1987) Canad. J. Microbiol., 33, pp. 162-168
  • Lehrach, Diamond, Wogney, Boedker, RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination (1977) Biochemistry, 16, pp. 4743-4751
  • Lindquist, The heat-shock response (1986) Annu. Rev. Biochem., 55, pp. 1151-1191
  • Medrano, Burrone, Ferrer, Cafferata, Algranati, A novel mechanism of resistance toα-difluoro-methylornithine induced by cycloheximide (1986) FEBS Lett., 206, pp. 106-110
  • Parnes, Velan, Felsenfeld, Ramanathan, Ferrini, Appella, Seidman, Mouse β2-microglobulin cDNA clones: A screening procedure for cDNA clones corresponding to rare mRNAs (1981) Proc. Natl. Acad. Sci. USA, 78, pp. 2253-2257
  • Perlman, Feldman, Cycloheximide and heat shock induce new polypeptide synthesis inNeurospora crassa (1982) Mol. Cell. Biol., 2, pp. 1167-1173
  • Plesofsky-Vig, Brambl, Heat shock response ofNeurospora crassa: Protein synthesis and induced thermotolerance (1985) J. Bacteriol., 162, pp. 1083-1091
  • Razin, Cedar, Riggs, (1984) DNA Methylation: Biochemistry and Biological Significance, , Springer-Verlag, New York
  • Reinert, Patel, Giles, Genetic regulation of theqa-1 function (1981) Mol. Cell. Biol., 1, pp. 829-835
  • Scott, Biochemical genetics of morphogenesis inNeurospora crassa (1976) Annu. Rev. Microbiol., 30, pp. 85-104
  • Selker, Stevens, Signal for DNA methylation associated with tandem duplication inNeurospora crassa (1987) Mol. Cell. Biol., 7, pp. 1032-1038
  • Takagi, Kawai, Takata, Tanaka, Sunairi, Miyazaki, Yano, Induction of cycloheximide resistance inCandida maltosa by modifying the ribosomes (1985) J. Gen. Appl. Microbiol., 31, pp. 267-275
  • Vogel, A convenient growth medium forNeurospora (medium N) (1956) Microb. Genet. Bull., 13, pp. 42-43
  • Westergaard, Mitchell, Neurospora V A synthetic medium favoring sexual reproduction (1947) American Journal of Botany, 34, pp. 573-577

Citas:

---------- APA ----------
Aisemberg, G.O., Grotewold, E., Taccioli, G.E. & Judewicz, N.D. (1989) . A major transcript in the response of Neurospora crassa to protein synthesis inhibition by cycloheximide. Experimental Mycology, 13(2), 121-128.
http://dx.doi.org/10.1016/0147-5975(89)90017-0
---------- CHICAGO ----------
Aisemberg, G.O., Grotewold, E., Taccioli, G.E., Judewicz, N.D. "A major transcript in the response of Neurospora crassa to protein synthesis inhibition by cycloheximide" . Experimental Mycology 13, no. 2 (1989) : 121-128.
http://dx.doi.org/10.1016/0147-5975(89)90017-0
---------- MLA ----------
Aisemberg, G.O., Grotewold, E., Taccioli, G.E., Judewicz, N.D. "A major transcript in the response of Neurospora crassa to protein synthesis inhibition by cycloheximide" . Experimental Mycology, vol. 13, no. 2, 1989, pp. 121-128.
http://dx.doi.org/10.1016/0147-5975(89)90017-0
---------- VANCOUVER ----------
Aisemberg, G.O., Grotewold, E., Taccioli, G.E., Judewicz, N.D. A major transcript in the response of Neurospora crassa to protein synthesis inhibition by cycloheximide. Exp. Mycol. 1989;13(2):121-128.
http://dx.doi.org/10.1016/0147-5975(89)90017-0