Abstract:
In Neurospora crassa grown in minimal medium with 2 μM cycloheximide, the expression of several genes was shown to be turned on. We have isolated one of those genes coding for a major transcript in this response, which newly appears after addition of cycloheximide and remains present at a high level for a long period. An open coding region for a 70-kDa protein was demonstrated by hybrid-selected translation, in agreement with the in vivo labeling of a protein with the same mobility in cycloheximide-induced cultures. Even 48 h after cycloheximide addition, CI-1-specific mRNA was bound to polysomal fractions. Our results also suggest that DNA sequences analogous to N. crassa CI-1 gene are present in other species. In N. crassa, CI-1 gene would not be induced by heat shock or starvation. © 1989.
Registro:
Documento: |
Artículo
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Título: | A major transcript in the response of Neurospora crassa to protein synthesis inhibition by cycloheximide |
Autor: | Aisemberg, G.O.; Grotewold, E.; Taccioli, G.E.; Judewicz, N.D. |
Filiación: | Instituto de Investigaciones en Ingenieria Genetica y Biologia Molecular INGEBI (CONICET), U.B.A. Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina Facultad de Ciencias Exactas y Naturales, U.B.A. Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina
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Palabras clave: | cycloheximide; gene expression; Neurospora crassa; stress proteins |
Año: | 1989
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Volumen: | 13
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Número: | 2
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Página de inicio: | 121
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Página de fin: | 128
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DOI: |
http://dx.doi.org/10.1016/0147-5975(89)90017-0 |
Título revista: | Experimental Mycology
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Título revista abreviado: | Exp. Mycol.
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ISSN: | 01475975
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CODEN: | EXMYD
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01475975_v13_n2_p121_Aisemberg |
Referencias:
- Aisemberg, Taccioli, Grotewold, Judewicz, Differential gene expression promoted by cycloheximide inNeurospora crassa (1987) Exp. Mycol., 11, pp. 122-127
- Aviv, Leder, Purification of biologically active globin messenger RNA by chromatography on oligo-thymidylic acid-cellulose (1972) Proc. Natl. Acad. Sci. USA, 69, pp. 1408-1412
- Chamberlain, Fluorographic detection of radioactivity in polyacrylamide gels with water-soluble fluors: Sodium salicylate (1979) Anal. Biochem., 98, pp. 132-135
- Fro¨ehner, Eriksson, Induction ofNeurospora crassa laccase with protein synthesis inhibitors (1974) J. Bacteriol., 120, pp. 450-457
- Horowitz, Evidence for common control of tyrosinase andl-amino-acid oxidase inNeurospora crassa (1965) Biochem. Biophys. Res. Commun., 18, pp. 686-692
- Horowitz, Feldman, Pall, Derepression of tyrosinase synthesis inNeurospora by cycloheximide actinomycin D and puromycin (1970) J. Biol. Chem., 245, pp. 2784-2788
- Horowitz, Fling, Macleod, Watanabe, Structural and regulative genes controlling tyrosinase synthesis inNeurospora (1961) Cold Spring Harbor Symp. Quant. Biol., 26, pp. 233-238
- Kapoor, Lewis, Alteration of the protein synthesis pattern inNeurospora crassa cells by hyperthermal and oxidative stress (1987) Canad. J. Microbiol., 33, pp. 162-168
- Lehrach, Diamond, Wogney, Boedker, RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination (1977) Biochemistry, 16, pp. 4743-4751
- Lindquist, The heat-shock response (1986) Annu. Rev. Biochem., 55, pp. 1151-1191
- Medrano, Burrone, Ferrer, Cafferata, Algranati, A novel mechanism of resistance toα-difluoro-methylornithine induced by cycloheximide (1986) FEBS Lett., 206, pp. 106-110
- Parnes, Velan, Felsenfeld, Ramanathan, Ferrini, Appella, Seidman, Mouse β2-microglobulin cDNA clones: A screening procedure for cDNA clones corresponding to rare mRNAs (1981) Proc. Natl. Acad. Sci. USA, 78, pp. 2253-2257
- Perlman, Feldman, Cycloheximide and heat shock induce new polypeptide synthesis inNeurospora crassa (1982) Mol. Cell. Biol., 2, pp. 1167-1173
- Plesofsky-Vig, Brambl, Heat shock response ofNeurospora crassa: Protein synthesis and induced thermotolerance (1985) J. Bacteriol., 162, pp. 1083-1091
- Razin, Cedar, Riggs, (1984) DNA Methylation: Biochemistry and Biological Significance, , Springer-Verlag, New York
- Reinert, Patel, Giles, Genetic regulation of theqa-1 function (1981) Mol. Cell. Biol., 1, pp. 829-835
- Scott, Biochemical genetics of morphogenesis inNeurospora crassa (1976) Annu. Rev. Microbiol., 30, pp. 85-104
- Selker, Stevens, Signal for DNA methylation associated with tandem duplication inNeurospora crassa (1987) Mol. Cell. Biol., 7, pp. 1032-1038
- Takagi, Kawai, Takata, Tanaka, Sunairi, Miyazaki, Yano, Induction of cycloheximide resistance inCandida maltosa by modifying the ribosomes (1985) J. Gen. Appl. Microbiol., 31, pp. 267-275
- Vogel, A convenient growth medium forNeurospora (medium N) (1956) Microb. Genet. Bull., 13, pp. 42-43
- Westergaard, Mitchell, Neurospora V A synthetic medium favoring sexual reproduction (1947) American Journal of Botany, 34, pp. 573-577
Citas:
---------- APA ----------
Aisemberg, G.O., Grotewold, E., Taccioli, G.E. & Judewicz, N.D.
(1989)
. A major transcript in the response of Neurospora crassa to protein synthesis inhibition by cycloheximide. Experimental Mycology, 13(2), 121-128.
http://dx.doi.org/10.1016/0147-5975(89)90017-0---------- CHICAGO ----------
Aisemberg, G.O., Grotewold, E., Taccioli, G.E., Judewicz, N.D.
"A major transcript in the response of Neurospora crassa to protein synthesis inhibition by cycloheximide"
. Experimental Mycology 13, no. 2
(1989) : 121-128.
http://dx.doi.org/10.1016/0147-5975(89)90017-0---------- MLA ----------
Aisemberg, G.O., Grotewold, E., Taccioli, G.E., Judewicz, N.D.
"A major transcript in the response of Neurospora crassa to protein synthesis inhibition by cycloheximide"
. Experimental Mycology, vol. 13, no. 2, 1989, pp. 121-128.
http://dx.doi.org/10.1016/0147-5975(89)90017-0---------- VANCOUVER ----------
Aisemberg, G.O., Grotewold, E., Taccioli, G.E., Judewicz, N.D. A major transcript in the response of Neurospora crassa to protein synthesis inhibition by cycloheximide. Exp. Mycol. 1989;13(2):121-128.
http://dx.doi.org/10.1016/0147-5975(89)90017-0