Abstract:
The effect of a protein synthesis inhibitor on gene expression inNeurospora crassa was analyzed as a model in which sexual differentiation is promoted. Using enriched cDNA probes we isolated several independent genomic clones which contained sequences of genes preferentially expressed when low amounts of cycloheximide were added to late stationary cultures. These clones hybridized to unique transcripts which belonged to the population of new RNA species generated in cycloheximide-treated mycelia. Several different transcripts were detected with enriched cDNA probes in Northern blots. © 1987 Academic Press, Inc.
Registro:
Documento: |
Artículo
|
Título: | Differential gene expression promoted by cycloheximide inNeurospora crassa |
Autor: | Aisemberg, G.O.; Taccioli, G.E.; Grotewold, E.; Judewicz, N.D. |
Filiación: | Instituto de Investigaciones en Ingenieri ́a Gene ́tica y Biologi ́a Molecular, INGEBI-CONICET, 1428 Buenos Aires, Argentina Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Obligado 2490, 1428 Buenos Aires, Argentina
|
Palabras clave: | differential gene expression; Neurospora crassa; protein synthesis inhibitors; sexual differentiation |
Año: | 1987
|
Volumen: | 11
|
Número: | 2
|
Página de inicio: | 122
|
Página de fin: | 127
|
DOI: |
http://dx.doi.org/10.1016/0147-5975(87)90045-4 |
Título revista: | Experimental Mycology
|
Título revista abreviado: | Exp. Mycol.
|
ISSN: | 01475975
|
CODEN: | EXMYD
|
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01475975_v11_n2_p122_Aisemberg |
Referencias:
- Aviv, Leder, Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose (1972) Proc. Natl. Acad. Sci. USA, 69, pp. 1408-1412
- Benton, Davis, Screening λgt recombinant clones by hybridization to single plaquesin situ (1977) Science, 196, pp. 180-182
- Berlin, Yanofsky, Isolation and characterization of genes differentially expressed during conidiation ofN. crassa (1985) Mol. Cell. Biol., 5, pp. 849-855
- Cruz, Terenzi, Derepression of tyrosinase by sexual stimulation (1981) Neurospora Newslett., 28, pp. 8-9
- Engelke, Ng, Shastry, Roeder, Specific interaction of a purified transcription factor with an internal control region of 5S RNA genes (1980) Cell, 19, pp. 717-728
- Froehner, Eriksson, Induction ofNeurospora crassa laccase with protein synthesis inhibitors (1974) J. Bacteriol., 120, pp. 450-457
- Horowitz, Evidence for common control of tyrosinase andl-amino-acid oxidase inNeurospora (1965) Biochem. Biophys. Res. Commun., 18, pp. 686-692
- Horowitz, Feldman, Pall, Derepression of tyrosinase synthesis inNeurospora by cycloheximide, actinomycin D and puromycin (1970) J. Biol. Chem., 245, pp. 2784-2788
- Horowitz, Fling, MacLeod, Watanabe, Structural and regulative genes controlling tyrosinase synthesis inNeurospora (1961) C. S. H. Symp. Quant. Biol., 26, pp. 233-238
- Katan, Galun, A rapid and efficient method for the purification of tyrosinase fromNeurospora crassa (1975) Anal. Biochem., 67, pp. 485-492
- Lehrach, Diamond, Wozney, Boedker, RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination (1977) Biochemistry, 16, pp. 4743-4751
- Reinert, Patel, Giles, Genetic regulation of theqa messenger ribonucleic acid and dependency onqa-1 function (1981) Mol. Cell. Biol., 1, pp. 829-835
- Rigby, Dieckman, Rhodes, Berg, Labelling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I (1977) J. Mol. Biol., 113, pp. 237-251
- Scott, Biochemical genetics of morphogenesis inNeurospora (1976) Annu. Rev. Microbiol., 30, pp. 85-104
- Sikora, Marzluff, Regulation ofl-aminoacid oxidase andd-aminoacid oxidase inN. crassa (1982) Mol. Gen. Genet., 186, pp. 33-39
- Tyler, Giles, Accurate transcription of clonedNeurospora RNA polymerase II dependent genesin vitro by homologous soluble extracts (1985) Proc. Natl. Acad. Sci. USA, 82, pp. 5450-5454
- Vogel, A convenient growth medium forNeurospora (Medium N) (1956) Microb. Genet. Bull., 13, pp. 42-43
- Yelton, Hamer, de Souza, Mullaney, Timberlake, Developmental regulation of theAspergillus nidulans trp c gene (1983) Proc. Natl. Acad. Sci. USA, 80, pp. 7576-7580
- Zimmerman, Orr, Leclerc, Barnard, Timberlake, Molecular cloning and selection of genes regulated inAspergillus development (1980) Cell, 21, pp. 709-715
Citas:
---------- APA ----------
Aisemberg, G.O., Taccioli, G.E., Grotewold, E. & Judewicz, N.D.
(1987)
. Differential gene expression promoted by cycloheximide inNeurospora crassa. Experimental Mycology, 11(2), 122-127.
http://dx.doi.org/10.1016/0147-5975(87)90045-4---------- CHICAGO ----------
Aisemberg, G.O., Taccioli, G.E., Grotewold, E., Judewicz, N.D.
"Differential gene expression promoted by cycloheximide inNeurospora crassa"
. Experimental Mycology 11, no. 2
(1987) : 122-127.
http://dx.doi.org/10.1016/0147-5975(87)90045-4---------- MLA ----------
Aisemberg, G.O., Taccioli, G.E., Grotewold, E., Judewicz, N.D.
"Differential gene expression promoted by cycloheximide inNeurospora crassa"
. Experimental Mycology, vol. 11, no. 2, 1987, pp. 122-127.
http://dx.doi.org/10.1016/0147-5975(87)90045-4---------- VANCOUVER ----------
Aisemberg, G.O., Taccioli, G.E., Grotewold, E., Judewicz, N.D. Differential gene expression promoted by cycloheximide inNeurospora crassa. Exp. Mycol. 1987;11(2):122-127.
http://dx.doi.org/10.1016/0147-5975(87)90045-4