Navegar

Colección

Datos Estadísticas

Artículo

Estamos trabajando para incorporar este artículo al repositorio
Consulte el artículo en la página del editor
Consulte la política de Acceso Abierto del editor

Abstract:

Natural carrageenans of diverse structural types isolated from the red seaweed Gigartina skottsbergii were recently identified as potent and selective inhibitors of herpes simplex virus types 1 and 2 (HSV-1 and -2). The μ/ν-carrageenan 1C3 was tested in vitro for its ability to select resistant variants. After serial passages of HSV-1 strain F in Vero cells in the presence of increasing concentrations of 1C3, viruses emerged that were approximately 2- to 10-fold more resistant to 1C3 inhibition than parental virus; these viruses formed large plaques with an altered syncytial phenotype (1C3-syn). Plaque-purified syncytial variants isolated from passages 13 and 14 have shown variable levels of resistance to 1C3, as well as to the other antiviral carrageenans isolated from G. skottsbergii and to other sulfated polysaccharides with known antiviral activity, such as heparin and dextran sulfate 8000, but all the clones were susceptible to acyclovir. The syn phenotype was not related to polysaccharide resistance. All the 1C3-syn variants formed large syncytia in Vero and CV-1 cells but did not induce fusion in other cell types. The growth efficiency in Vero cells, as well as the virulence for mice by intracerebral or intraperitoneal inoculation of 1C3-syn variants, showed no significant alterations in comparison with the parental virus. The syncytial properties were not affected by cyclosporine or melittin, suggesting that an alteration on glycoprotein gB could be responsible for the syn phenotype induced by 1C3. © 2002 Wiley-Liss, Inc.

Registro:

Documento: Artículo
Título:Herpes simplex virus type 1 variants arising after selection with an antiviral carrageenan: Lack of correlation between drug susceptibility and syn phenotype
Autor:Carlucci, M.J.; Scolaro, L.A.; Damonte, E.B.
Filiación:Laboratorio de Virología, Departamento de Química Biológica, Ciudad Universitaria, Buenos Aires, Argentina
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Pabellón 2, 1428 Buenos Aires, Argentina
Palabras clave:Antiviral; Antiviral resistance; Carrageenan; Herpesvirus; Syncytial variant; aciclovir; antivirus agent; carrageenan; cyclosporin; dextran sulfate; glycoprotein; heparin; melittin; polysaccharide; antivirus agent; carrageenan; animal cell; animal experiment; animal model; antiviral activity; article; cell fusion; concentration response; controlled study; drug sensitivity; Herpes simplex virus 1; Herpes simplex virus 2; human; human cell; mouse; nonhuman; phenotype; phenotypic variation; seaweed; Vero cell; virogenesis; virus inhibition; virus plaque; virus resistance; virus virulence; animal; cell culture; Cercopithecus; disease model; drug effect; genetic variability; giant cell; herpes simplex; pathogenicity; phenotype; physiology; virology; Animal; Antiviral Agents; Carrageenan; Cercopithecus aethiops; Disease Models, Animal; Giant Cells; Herpes Simplex; Herpesvirus 1, Human; Human; Mice; Phenotype; Support, Non-U.S. Gov't; Tumor Cells, Cultured; Variation (Genetics); Vero Cells; Animals; Cercopithecus aethiops; Disease Models, Animal; Giant Cells; Humans; Mice; Phenotype; Tumor Cells, Cultured; Variation (Genetics); Vero Cells; algae; Gigartina; Gigartina skottsbergii; Herpesviridae; Human herpesvirus 1; Simplexvirus
Año:2002
Volumen:68
Número:1
Página de inicio:92
Página de fin:98
DOI: http://dx.doi.org/10.1002/jmv.10174
Título revista:Journal of Medical Virology
Título revista abreviado:J. Med. Virol.
ISSN:01466615
CODEN:JMVID
CAS:Carrageenan, 9000-07-1; Antiviral Agents
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01466615_v68_n1_p92_Carlucci

Referencias:

  • Baba, M., Snoeck, R., Pauwels, R., De Clercq, E., Sulfated polysaccharides are potent and selective inhibitors of various enveloped viruses, including herpes simplex virus, cytomegalovirus, vesicular stomatitis virus and human immunodeficiency virus (1988) Antimicrob Agents Chemother, 32, pp. 1742-1745
  • Baghian, A., Kousoulas, K.G., Role of the Na + , K + pump in herpes simplex type 1-induced cell fusion: Melittin causes specific reversion of syncytial mutants with the syn 1 mutation to syn + (wild-type) phenotype (1993) Virology, 196, pp. 548-556
  • Carlucci, M.J., Pujol, C.A., Ciancia, M., Noseda, M.D., Matulewicz, M.C., Damonte, E.B., Cerezo, A.S., Antiherpetic and anticoagulant properties of carrageenans from the red seaweed Gigartina skottsbergii and their cyclized derivatives: Correlation between structure and biological activity (1997) Int J Biol Macromol, 20, pp. 97-105
  • Carlucci, M.J., Ciancia, M., Matulewicz, M.C., Cerezo, A.S., Damonte, E.B., Antiherpetic activity and mode of action of natural carrageenans of diverse structural types (1999) Antiviral Res, 43, pp. 93-102
  • Carlucci, M.J., Scolaro, L.A., Damonte, E.B., Inhibitory action of natural carrageenans on herpes simplex virus infection of mouse astrocytes (1999) Chemotherapy, 45, pp. 429-436
  • Christophers, J., Clayton, J., Craske, J., Ward, R., Collins, P., Trowbridge, M., Darby, G., Survey of resistance of herpes simplex virus to acyclovir in northwest England (1998) Antimicrob Agents Chemother, 42, pp. 862-872
  • Coen, D.M., Acyclovir-resistant, pathogenic herpesviruses (1994) Trends Microbiol, 2, pp. 481-485
  • Crumpacker, C.S., Schnipper, L.E., Marlowe, S.I., Kowalsky, P.N., Hershey, B.J., Levin, M.J., Resistance to antiviral drugs of herpes simplex virus isolated from a patient treated with acyclovir (1982) N Engl J Med, 306, pp. 343-346
  • Darby, G., In search of the perfect antiviral (1995) Antiviral Chem Chemother, 6 (SUPPL. 1), pp. 54-63
  • Field, A.K., Biron, K.K., The end of innocence revisited: Resistance of herpesviruses to antiviral drugs (1994) Clin Microbiol Rev, 7, pp. 1-13
  • Gage, P.J., Levine, M., Glorioso, J.C., Syncytium-inducing mutations localize to two discrete regions within the cytoplasmic domain of herpes simplex virus type 1 glycoprotein B (1993) J Virol, 67, pp. 2191-2201
  • Goodman, J.L., Engel, J.P., Altered pathogenesis in herpes simplex virus type 1 infection due to a syncytial mutation mapping to the carboxy terminus of glycoprotein B (1991) J Virol, 65, pp. 1770-1778
  • Herold, B.C., WuDunn, D., Visalli, R.J., Sumarski, N., Brandt, C., Spear, P.G., Glycoprotein C-independent binding of herpes simplex virus to cells requires cell surface heparan sulfate and glycoprotein B (1994) J Gen Virol, 75, pp. 1211-1222
  • Herold, B.C., Gerber, S.I., Polonsky, T., Belval, B.J., Shaklee, P.N., Holme, K., Identification of structural features of heparin required for inhibition of herpes simplex virus type 1 binding (1995) Virology, 206, pp. 1108-1116
  • Hutchinson, L., Goldsmith, K., Snoddy, D., Ghosh, H., Graham, F.L., Johnson, D.C., Identification and characterization of a novel herpes simplex glycoprotein, gK, involved in cell fusion (1992) J Virol, 66, pp. 5603-5609
  • Hutchinson, L., Roop-Beauchamp, C., Johnson, D.C., Herpes simplex virus glycoprotein K is known to influence fusion of infected cells, yet is not on the cell surface (1995) J Virol, 69, pp. 4556-4563
  • Kimberlin, D.W., Whitley, R.J., Antiviral resistance: Mechanisms, clinical significance, and future implications (1996) J Antimicrob Chem, 37, pp. 403-421
  • Lingen, M., Seck, T., Weise, K., Falke, D., Single amino acid substitutions in the glycoprotein B carboxy terminus influence the fusion from without property of herpes simplex virus type 1 (1995) J Gen Virol, 76, pp. 1843-1849
  • Padilla, J., Yamada, M., Takahashi, Y., Tsukazaki, T., Nakamura, J., Yoshida, M., Uno, F., Nii, S., In vitro selection of variants of herpes simplex virus type 1 which differ in cytopathic changes (1997) Microbiol Immunol, 41, pp. 203-207
  • Pertel, P.E., Spear, P.G., Modified entry and syncytium formation by herpes simplex virus type 1 mutants selected for resistance to heparin inhibition (1996) Virology, 226, pp. 22-33
  • Safrin, S., Crumpacker, C., Chatis, P., Davis, R., Hafner, R., Rush, J., Kessler, H.A., Mills, J., A controlled trial comparing foscarnet with vidarabine for acyclovir-resistant mucocutaneous herpes simplex in the acquired immunodeficiency syndrome (1991) N Engl J Med, 325, pp. 551-555
  • Sanders, P.G., Wilkie, N.M., Davison, A.J., Thymidine kinase deletion mutants of herpes simplex virus type 1 (1982) J Gen Virol, 63, pp. 277-295
  • Tal-Singer, R., Peng, C., Ponce de Leon, M., Abrams, W.R., Banfield, B.W., Tufaro, F., Cohen, G.H., Eisenberg, R.J., Interaction of herpes simplex virus glycoprotein gC with mammalian cell surface molecules (1995) J Virol, 69, pp. 4471-4483
  • Tognon, M., Guandalini, R., Romanelli, M.G., Manservigi, R., Trevisani, B., Phenotypic and genotypic characterization of locus Syn 5 in herpes simplex virus 1 (1991) Virus Res, 18, pp. 135-150
  • Trybala, E., Liljequist, J., Svennerholm, B., Bergstrom, T., Herpes simplex virus types 1 and 2 differ in their interaction with heparan sulfate (2000) J Virol, 74, pp. 9106-9114
  • Wade, J.C., Newton, B., McLaren, C., Flournoy, R.E., Meyers, J.D., Intravenous acyclovir to treat mucocutaneous herpes simplex virus infection after marrow transplantation: A double blind test (1983) Ann Intern Med, 96, pp. 265-269
  • Walev, I., Lingen, M., Lázaro, M., Weise, K., Falke, D., Cyclosporin A resistance of herpes simplex virus-induced "fusion from within" as a phenotypical marker of mutations in the syn 3 locus of the glycoprotein B gene (1994) Virus Genes, 8, pp. 83-86
  • Witvrouw, M., De Clercq, E., Sulfated polysaccharides extracted from sea algae as potential antiviral drugs (1997) Gen Pharmacol, 29, pp. 497-511
  • Witvrouw, M., Desmyter, J., De Clercq, E., Antiviral portrait series. 4. Polysulfates as inhibitors of HIV and other enveloped viruses (1994) Antiviral Chem Chemother, 5, pp. 345-359

Citas:

---------- APA ----------
Carlucci, M.J., Scolaro, L.A. & Damonte, E.B. (2002) . Herpes simplex virus type 1 variants arising after selection with an antiviral carrageenan: Lack of correlation between drug susceptibility and syn phenotype. Journal of Medical Virology, 68(1), 92-98.
http://dx.doi.org/10.1002/jmv.10174
---------- CHICAGO ----------
Carlucci, M.J., Scolaro, L.A., Damonte, E.B. "Herpes simplex virus type 1 variants arising after selection with an antiviral carrageenan: Lack of correlation between drug susceptibility and syn phenotype" . Journal of Medical Virology 68, no. 1 (2002) : 92-98.
http://dx.doi.org/10.1002/jmv.10174
---------- MLA ----------
Carlucci, M.J., Scolaro, L.A., Damonte, E.B. "Herpes simplex virus type 1 variants arising after selection with an antiviral carrageenan: Lack of correlation between drug susceptibility and syn phenotype" . Journal of Medical Virology, vol. 68, no. 1, 2002, pp. 92-98.
http://dx.doi.org/10.1002/jmv.10174
---------- VANCOUVER ----------
Carlucci, M.J., Scolaro, L.A., Damonte, E.B. Herpes simplex virus type 1 variants arising after selection with an antiviral carrageenan: Lack of correlation between drug susceptibility and syn phenotype. J. Med. Virol. 2002;68(1):92-98.
http://dx.doi.org/10.1002/jmv.10174