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Abstract:

Herpetic stromal keratitis is caused by ocular infection with herpes simplex virus type 1 (HSV-1) and constitutes a leading cause of human blindness. The effect of meliacine, an antiviral compound isolated from leaves of Melia azedarach L. that inhibits HSV-1 replication in vitro, was examined on experimental corneal HSV-1 inoculation in Balb/c mice. Mice were inoculated with HSV-1 strain KOS at their corneas after abrasion. Meliacine was administered topically 3 times a day for 4 days beginning 1 day before inoculation. Infected animals treated or not with meliacine were observed carefully for the development of stromal keratitis and the clinical scoring was done 14 days post-infection. Histological examination of corneas and viral isolation from eyes from HSV-1 infected mice treated or not with meliacine were also carried out. It was found that the treatment of HSV-1- induced ocular disease in Balb/c mice with meliacine reduced significantly the development of clinical disease, as well as the histological damage in corneas. The viral titers detected in eyes of infected and treated mice were 2-orders-of-magnitude lower than those corresponding to HSV-1 infected control animals. Mock-infected and treated mice did not reveal any corneal alteration due to the administration of the compound. Meliacine was found to exert a strong antiviral action on HSV-1-induced ocular disease in mice with no evidence of toxic effects. (C) 2000 Wiley-Liss, Inc.

Registro:

Documento: Artículo
Título:Treatment with meliacine, a plant derived antiviral, prevents the development of herpetic stromal keratitis in mice
Autor:Alché, L.E.; Berra, A.; Veloso, M.J.; Coto, C.E.
Filiación:Laboratory of Virology, Department of Biochemistry, School of Science, Buenos Aires, Argentina
Department of Pathology, School of Medicine, University of Buenos Aires, Buenos Aires, Argentina
Lab. de Virología, Depto. de Quim. Biológica, Ciudad Universitaria, Pabellón II, 1428 Buenos Aires, Argentina
Palabras clave:Herpes simplex virus type 1; Herpetic stromal keratitis; Melia azedarach L; Plant antiviral; antivirus agent; meliacine; unclassified drug; animal experiment; animal model; animal tissue; antimicrobial therapy; antiviral activity; article; controlled study; drug effect; female; herpes simplex keratitis; Herpes simplex virus 1; histopathology; male; mouse; nonhuman; plant leaf; scoring system; virus isolation; virus titration
Año:2000
Volumen:61
Número:4
Página de inicio:474
Página de fin:480
DOI: http://dx.doi.org/10.1002/1096-9071(200008)61:4<474::AID-JMV10>3.0.CO;2-K
Título revista:Journal of Medical Virology
Título revista abreviado:J. Med. Virol.
ISSN:01466615
CODEN:JMVID
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01466615_v61_n4_p474_Alche

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Citas:

---------- APA ----------
Alché, L.E., Berra, A., Veloso, M.J. & Coto, C.E. (2000) . Treatment with meliacine, a plant derived antiviral, prevents the development of herpetic stromal keratitis in mice. Journal of Medical Virology, 61(4), 474-480.
http://dx.doi.org/10.1002/1096-9071(200008)61:4<474::AID-JMV10>3.0.CO;2-K
---------- CHICAGO ----------
Alché, L.E., Berra, A., Veloso, M.J., Coto, C.E. "Treatment with meliacine, a plant derived antiviral, prevents the development of herpetic stromal keratitis in mice" . Journal of Medical Virology 61, no. 4 (2000) : 474-480.
http://dx.doi.org/10.1002/1096-9071(200008)61:4<474::AID-JMV10>3.0.CO;2-K
---------- MLA ----------
Alché, L.E., Berra, A., Veloso, M.J., Coto, C.E. "Treatment with meliacine, a plant derived antiviral, prevents the development of herpetic stromal keratitis in mice" . Journal of Medical Virology, vol. 61, no. 4, 2000, pp. 474-480.
http://dx.doi.org/10.1002/1096-9071(200008)61:4<474::AID-JMV10>3.0.CO;2-K
---------- VANCOUVER ----------
Alché, L.E., Berra, A., Veloso, M.J., Coto, C.E. Treatment with meliacine, a plant derived antiviral, prevents the development of herpetic stromal keratitis in mice. J. Med. Virol. 2000;61(4):474-480.
http://dx.doi.org/10.1002/1096-9071(200008)61:4<474::AID-JMV10>3.0.CO;2-K