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Abstract:

PURPOSE. To study the effect of aminoguanidine (AMG), an inhibitor of nitric oxide production, on the ocular infection of Balb/c mice with herpes simplex virus (HSV) type 1 strain F and HSV-2 strain G. METHODS. Animals were treated with different amounts of AMG (0.5, O. 1, and 0.05 mg/mouse) by topical application in the eye from postinfection (PI) days -2 through +5, considering 0 the day of infection. At different PI days, development of herpetic keratitis was evaluated in treated and control mice. RESULTS. Treated animals showed a dose-dependent increase in ocular disease after viral infection, compared with control animals. Viral titers in ocular washings were higher in AMG-treated mice (PI day 2, HSV-1: AMG 0.5 mg, 1.3 × 103 plaque-forming units (PFU)/ml; control, O. 22 × 102 PFU/ml, P < 0.025). At PI day 3, control corneas had only scattered inflammatory cells, whereas those from treated animals showed a conspicuous infiltrate consisting primarily of neutrophils. Viral titers were also higher in brains of treated mice. These animals died earlier and in a greater proportion than control animals (percentage of mortality, PI day 12, HSV-1: AMG 0.5 mg, 40% ± 4%; control, 18% ± 3%, P < 0.05). CONCLUSIONS. These data indicate an inhibitory effect of nitric oxide on HSV ocular infection.

Registro:

Documento: Artículo
Título:Effect of aminoguanidine, a nitric oxide synthase inhibitor, on ocular infection with herpes simplex virus in Balb/c mice
Autor:Benencia, F.; Courrèges, M.C.; Gamba, G.; Cavalieri, H.; Massouh, E.J.
Filiación:Laboratorio de Inmunoquímica, Dpto. Química Biologica, Universidad de Buenos Aires, Ciudad Universitaria, 1428 Buenos Aires, Argentina
Palabras clave:aminoguanidine; nitric oxide; nitric oxide synthase inhibitor; animal experiment; animal model; animal tissue; article; controlled study; cornea; dose response; eye infection; Herpes simplex virus 1; Herpes simplex virus 2; male; mouse; mouse strain; neutrophil; nonhuman; priority journal; virus titration; Administration, Topical; Animals; Cornea; Disease Progression; DNA, Viral; Dose-Response Relationship, Drug; Enzyme Inhibitors; Guanidines; Herpesvirus 1, Human; Herpesvirus 2, Human; Keratitis, Herpetic; Mice; Mice, Inbred BALB C; Nitric Oxide Synthase; Ophthalmic Solutions; Reverse Transcriptase Polymerase Chain Reaction; Trigeminal Ganglion
Año:2001
Volumen:42
Número:6
Página de inicio:1277
Página de fin:1284
Título revista:Investigative Ophthalmology and Visual Science
Título revista abreviado:Invest. Ophthalmol. Vis. Sci.
ISSN:01460404
CODEN:IOVSD
CAS:DNA, Viral; Enzyme Inhibitors; Guanidines; Nitric Oxide Synthase, EC 1.14.13.39; Ophthalmic Solutions; pimagedine, 79-17-4
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01460404_v42_n6_p1277_Benencia

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Citas:

---------- APA ----------
Benencia, F., Courrèges, M.C., Gamba, G., Cavalieri, H. & Massouh, E.J. (2001) . Effect of aminoguanidine, a nitric oxide synthase inhibitor, on ocular infection with herpes simplex virus in Balb/c mice. Investigative Ophthalmology and Visual Science, 42(6), 1277-1284.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01460404_v42_n6_p1277_Benencia [ ]
---------- CHICAGO ----------
Benencia, F., Courrèges, M.C., Gamba, G., Cavalieri, H., Massouh, E.J. "Effect of aminoguanidine, a nitric oxide synthase inhibitor, on ocular infection with herpes simplex virus in Balb/c mice" . Investigative Ophthalmology and Visual Science 42, no. 6 (2001) : 1277-1284.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01460404_v42_n6_p1277_Benencia [ ]
---------- MLA ----------
Benencia, F., Courrèges, M.C., Gamba, G., Cavalieri, H., Massouh, E.J. "Effect of aminoguanidine, a nitric oxide synthase inhibitor, on ocular infection with herpes simplex virus in Balb/c mice" . Investigative Ophthalmology and Visual Science, vol. 42, no. 6, 2001, pp. 1277-1284.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01460404_v42_n6_p1277_Benencia [ ]
---------- VANCOUVER ----------
Benencia, F., Courrèges, M.C., Gamba, G., Cavalieri, H., Massouh, E.J. Effect of aminoguanidine, a nitric oxide synthase inhibitor, on ocular infection with herpes simplex virus in Balb/c mice. Invest. Ophthalmol. Vis. Sci. 2001;42(6):1277-1284.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01460404_v42_n6_p1277_Benencia [ ]