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Abstract:

The thermal stability of the neutral protease of Aspergillus oryzae was measured by differential scanning calorimetry. The activation energy, preexponential factor and the reaction rate constant calculated by means of the dynamic method are similar to those obtained for denaturation of other proteins. Half life (t1/2) calculated by using the above‐mentioned parameters permitted estimation of the amount of native enzyme remaining after different thermal treatments. Complete denaturation occurred above 60C. The presence of substrate stabilizes the enzyme. The behavior of flour samples treated with the neutral protease was studied as well. A tendency to shift towards higher temperatures when flour was treated with the enzyme was observed for both the Tp (DSC peak maximum temperature) corresponding to gelatinization of starch and the dissociation of the amylose‐lipid complex. Copyright © 1994, Wiley Blackwell. All rights reserved

Registro:

Documento: Artículo
Título:THERMAL STABILITY OF A NEUTRAL PROTEASE OF ASPERGILLUS ORYZAE
Autor:BOMBARA, N.; PILOSOF, A.M.R.; AÑÓN, M.C.
Filiación:Departamento de Industrias, Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria, Buenos Aires, 1428, Argentina
Palabras clave:Aspergillus oryzae
Año:1994
Volumen:18
Número:1
Página de inicio:31
Página de fin:41
DOI: http://dx.doi.org/10.1111/j.1745-4514.1994.tb00487.x
Título revista:Journal of Food Biochemistry
Título revista abreviado:J. Food Biochem.
ISSN:01458884
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01458884_v18_n1_p31_BOMBARA

Referencias:

  • ADLER‐NISSEN, J., OLSEN, H.S., The influence of peptide chain length on taste and functional properties of enzymatically modified soy protein (1979) ACS Symp. Ser., 92, pp. 115-146
  • BARRET, F.F., Enzyme uses in the milling and baking industries (1966) Enzymes in Food Processing, pp. 301-330. , G. Reed, Academic Press, New York
  • BATEY, I.L., Enzymatic solubilization of wheat gluten (1985) J. Appl. Biochem., 7, pp. 423-429
  • BOMBARA, N., PILOSOF, A.M.R., AÑÒN, M.C., Kinetics of wheat proteins solubilization with a fungal protease (1992) Lebensm. Wiss. Technol., 25, pp. 527-531
  • ELIASSON, A.C., Differential scanning calorimetry studies on wheat starchgluten mixtures (1983) I. Effect of gluten on gelatinization of wheat starch. J. Cereal Sci., 1, pp. 119-205
  • ELIASSON, A.C., Some physico‐chemical properties of wheat starch (1989) Proceedings from ICC ′89 Wheat End Use Properties — Wheat and Flour Characterization for Specific End‐Uses, pp. 355-365. , Compiled by, H. Salovaara, Lakti
  • GAUTHIER, S.F., PAQUIN, P., POULIOT, Y., TURGEON, S., Emulsifying and oil‐binding properties of bovine serum albumin and its enzymatic hydrolyzate (1993) Biosci. Biotechnol. Biochem., 57, pp. 952-956
  • GHIASI, K., VARRIANO‐MARSTON, E., HOSENEY, R.C., Gelatinization of wheat starch. II. Starch‐surfactant interaction (1982) Cereal Chem., 59, pp. 86-88
  • GRUNDEN, L.P., VADEHRA, D.V., BAKER, R.C., Effects of proteolytic enzymes on the functionality of chicken egg albumen (1974) Journal of Food Science, 39, pp. 841-843
  • JANKOWSKI, T., RHA, C.K., Differential scanning calorimetry study of the wheat grain cooking process (1986) Starch - Stärke, 38, pp. 45-48
  • JAVANOVICH, G., ZAMPONI, R.A., LUPANO, C.E., AÑÒN, M.C., Effect of water content on the formation and dissociation of the amylose‐lipid complex in wheat flour (1992) Journal of Agricultural and Food Chemistry, 40, pp. 1789-1793
  • KATO, A., SHIMOKAWA, K., KOBAYASHI, K., Improvement of functional properties of insoluble gluten by promase digestion followed by dextran conjungation‐wheat gluten treatment with promase‐E for protein solubilization for use in food (1991) J. Agric. Food Chem., 39, pp. 1053-1056
  • KUGIMIYA, M., DONOVAN, J.W., WONG, R.Y., Phase transitions of amylose‐lipid complexes in starches: a calorimetric studies (1980) Starch - Stärke, 32, pp. 265-270
  • LUPANO, C.E., AÑÒN, M.C., Denaturation of wheat germ proteins during drying (1986) Cereal Chem., 63, pp. 259-263
  • LYOONS, T.P., Proteinase enzymes relevant to the baking industry (1982) Biochemical Society Transactions, 10, pp. 287-290
  • MARTENS, H., STABURSVIK, E., MARTENS, M., Texture and colour changes in meat during cooking related to thermal denaturation of muscle proteins (1982) Journal of Texture Studies, 13, pp. 291-309
  • NORM ASTM 474/80. Calibration temperature scale for differential thermal analysis; NORM ANSI‐ASTM 698/79 Standard test method for Arrhenius kinetic constants for thermally unstable materials; OZAWA, T., Kinetic analysis of derivative curves in thermal analysis (1970) J. Thermal Anal., 2, pp. 301-324
  • PELLETIER, G.E., QUELLET, L., Influence of pH and temperature on myosin inactivation (1961) Canadian Journal of Chemistry, 39, pp. 265-272
  • PUSKI, G., Modification of functional properties of soy proteins by proteolytic enzyme treatment (1975) Cereal Chem., 52, pp. 655-664
  • SAITO, M., HIKUNI, K., MONMA, M., SHIMIZU, M., Emulsifying and oil‐binding properties of bovine serum albumin and its enzymatic hydrolyzate (1993) Biosci. Biotechnol. Biochem., 57, pp. 952-956

Citas:

---------- APA ----------
BOMBARA, N., PILOSOF, A.M.R. & AÑÓN, M.C. (1994) . THERMAL STABILITY OF A NEUTRAL PROTEASE OF ASPERGILLUS ORYZAE. Journal of Food Biochemistry, 18(1), 31-41.
http://dx.doi.org/10.1111/j.1745-4514.1994.tb00487.x
---------- CHICAGO ----------
BOMBARA, N., PILOSOF, A.M.R., AÑÓN, M.C. "THERMAL STABILITY OF A NEUTRAL PROTEASE OF ASPERGILLUS ORYZAE" . Journal of Food Biochemistry 18, no. 1 (1994) : 31-41.
http://dx.doi.org/10.1111/j.1745-4514.1994.tb00487.x
---------- MLA ----------
BOMBARA, N., PILOSOF, A.M.R., AÑÓN, M.C. "THERMAL STABILITY OF A NEUTRAL PROTEASE OF ASPERGILLUS ORYZAE" . Journal of Food Biochemistry, vol. 18, no. 1, 1994, pp. 31-41.
http://dx.doi.org/10.1111/j.1745-4514.1994.tb00487.x
---------- VANCOUVER ----------
BOMBARA, N., PILOSOF, A.M.R., AÑÓN, M.C. THERMAL STABILITY OF A NEUTRAL PROTEASE OF ASPERGILLUS ORYZAE. J. Food Biochem. 1994;18(1):31-41.
http://dx.doi.org/10.1111/j.1745-4514.1994.tb00487.x