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Alvarez, M.G.; Milanesio, M.; Rivarola, V.; Durantini, E.; Batlle, A.; Fukuda, H. "Endogenous and exogenous porphyrins as photosensitizers in the Hep-2 human carcinoma cell line" (2009) Cellular and Molecular Biology. 55(2):8-14
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Abstract:

The photodynamic activity of three photosensitizers (PS): ALA-induced PPIX, the porphyrin derivative 5-(4-trimethylammoniumphenyl)-10, 5, 20-tris (2,4,6- trimethoxyphenyl) porphyrin (CP) and the molecular dyad porphyrin-C60 (P-C60), the last two incorporated into liposomal vesicles, was evaluated on Hep-2 human larynx carcinoma cell line. ALAinduced accumulation of the endogenous PS PPIX, reached saturation values between 5 and 24 h incubation time; the maximal PPIX content was 5.7 nmol/106 cells. The same intracellular level was accumulated when the cationic porphyrin CP was used, while the amount of P-C60 attained was 1.5 nmol/106 cells. Under violet-blue exciting light, the fluorescence of PPIX and P-C60 was found in the cytoplasm showing a granular appearance indicating lysosomal localization. CP was mainly detected as a filamentous pattern characteristic of mitochondrial localization. No dark cytotoxicity was observed using 1mM ALA, 5 μM CP and 1 μM P-C60 after 24 h incubation. Cell morphology was analyzed using Hoechst-33258, toluidine blue staining, TUNEL assay and DNA fragmentation, 24 h after irradiation with 54 J/cm2. When photosensitized with ALA and P-C60, chromatine condensation characteristic of apoptotic cell death was found; instead, 58 % of necrotic cells were observed with CP. The results show that in the Hep-2 cells, of the three PS analyzed, the molecular dyad P-C60 was more efficient than CP and PPIX, and confirm that PDT can induce different mechanisms of cell death depending on the PS and the irradiation dose. Copyright © 2009 C.M.B. Edition.

Registro:

Documento: Artículo
Título:Endogenous and exogenous porphyrins as photosensitizers in the Hep-2 human carcinoma cell line
Autor:Alvarez, M.G.; Milanesio, M.; Rivarola, V.; Durantini, E.; Batlle, A.; Fukuda, H.
Filiación:Departamento de Biología Molecular, Universidad Nacional de Río Cuarto, Argentina
Departamento de Química, Universidad Nacional de Río Cuarto, Argentina
Centro de Investigaciones Sobre Porfirinas Y Porfirias (CIPYP), CONICET, Argentina
Departamento de Química Biológica, FCEN, Universidad de Buenos Aires, Argentina
Palabras clave:5 aminolevulinic acid; Apoptosis; Cancer; Necrosis; Photodynamic therapy; Photosensitization; Phototherapeutic agents; PPIX; 5 (4 trimethylammoniumphenyl) 10,5,20 tris (2,4,6 trimethoxyphenyl) porphyrin; antineoplastic agent; unclassified drug; apoptosis; article; cancer cell culture; cell death; cell structure; cellular distribution; controlled study; cytoplasm; HEp 2 cell; human; human cell; photodynamic therapy; photosensitization; Aminolevulinic Acid; Carcinoma; Cell Line, Tumor; Cell Survival; Fullerenes; Humans; Laryngeal Neoplasms; Light; Liposomes; Microscopy, Fluorescence; Photochemotherapy; Photosensitizing Agents; Porphyrins; Protoporphyrins
Año:2009
Volumen:55
Número:2
Página de inicio:8
Página de fin:14
DOI: http://dx.doi.org/10.1170/T846
Título revista:Cellular and Molecular Biology
Título revista abreviado:Cell. Mol. Biol.
ISSN:01455680
CODEN:CMBID
CAS:Aminolevulinic Acid, 88755TAZ87; Fullerenes; Liposomes; Photosensitizing Agents; Porphyrins; Protoporphyrins; fullerene C60, NP9U26B839; protoporphyrin IX, 553-12-8
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01455680_v55_n2_p8_Alvarez

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Citas:

---------- APA ----------
Alvarez, M.G., Milanesio, M., Rivarola, V., Durantini, E., Batlle, A. & Fukuda, H. (2009) . Endogenous and exogenous porphyrins as photosensitizers in the Hep-2 human carcinoma cell line. Cellular and Molecular Biology, 55(2), 8-14.
http://dx.doi.org/10.1170/T846
---------- CHICAGO ----------
Alvarez, M.G., Milanesio, M., Rivarola, V., Durantini, E., Batlle, A., Fukuda, H. "Endogenous and exogenous porphyrins as photosensitizers in the Hep-2 human carcinoma cell line" . Cellular and Molecular Biology 55, no. 2 (2009) : 8-14.
http://dx.doi.org/10.1170/T846
---------- MLA ----------
Alvarez, M.G., Milanesio, M., Rivarola, V., Durantini, E., Batlle, A., Fukuda, H. "Endogenous and exogenous porphyrins as photosensitizers in the Hep-2 human carcinoma cell line" . Cellular and Molecular Biology, vol. 55, no. 2, 2009, pp. 8-14.
http://dx.doi.org/10.1170/T846
---------- VANCOUVER ----------
Alvarez, M.G., Milanesio, M., Rivarola, V., Durantini, E., Batlle, A., Fukuda, H. Endogenous and exogenous porphyrins as photosensitizers in the Hep-2 human carcinoma cell line. Cell. Mol. Biol. 2009;55(2):8-14.
http://dx.doi.org/10.1170/T846