Artículo
Diez, B.; Cordo Russo, R.; Teijo, M.J.; Hajos, S.; Batlle, A.; Fukuda, H. "ROS production by endogenously generated protoporphyrin IX in murine leukemia cells" (2009) Cellular and Molecular Biology. 55(2):15-19
Estamos trabajando para conseguir la versión final de este artículo
Consulte la política de Acceso Abierto del editor
Abstract:
Endogenous production of Protoporphyrin IX (PpIX) is successfully exploited for photodynamic therapy (PDT) on malignant cells, following 5-aminolevulinic acid (ALA) administration and light irradiation. This treatment kills cancer cells by damaging organelles and impairing metabolic pathways via cellular reactive oxygen species (ROS) generation. We studied the efficiency of PpIX synthetized from ALA on ROS generation, in the Vincristine resistant (LBR-V160), Doxorubicin resistant (LBR-D160) and sensitive (LBR-) murine leukemia cell lines. Cells were incubated 4 hr with 1 mM ALA and then irradiated during different times with fluorescent light. One hour later, production of ROS was analyzed by flow cytometry using different fluorescent probes: Hydroethidine (HE) for superoxide anion, 2',7' Dichlorodihydrofluorescein diacetate (DCFH-DA) for hydrogen peroxide; mitochondrial damage was examined with 3,3' Dihexyloxacarbocyanine iodide (DiOC6). We found that superoxide anion production in the three cell lines increased with irradiation time whereas no peroxide hydrogen was detected. Mitochondrial damage also increased in an irradiation time dependent manner, being higher in the Vincristine resistant line. Previous studies have demonstrated that apoptotic cell death increased with irradiation time, which is consistent with these results, indicating that ROS are critical in ALA-PDT efficiency to kill malignant cells. Copyright © 2009 C.M.B. Edition.
Registro:
| Documento: | Artículo |
| Título: | ROS production by endogenously generated protoporphyrin IX in murine leukemia cells |
| Autor: | Diez, B.; Cordo Russo, R.; Teijo, M.J.; Hajos, S.; Batlle, A.; Fukuda, H. |
| Filiación: | Centro de Investigaciones Sobre Porfirinas Y Porfirias (CIPYP), CONICET, UBA, Argentina Departamento de Química Biológica, FCEN, UBA, Argentina Cátedra de Inmunología, IDEHU, UBA - CONICET, Argentina Viamonte 1881-10A, 1056-Buenos Aires, Argentina |
| Palabras clave: | 5-aminolevulinic acid; Photodynamic therapy; Porphyrins; aminolevulinic acid; dichlorodihydrofluorescein diacetate; doxorubicin; hydroethidine; protoporphyrin; reactive oxygen metabolite; superoxide; vincristine; apoptosis; article; cancer cell culture; cell damage; controlled study; flow cytometry; human; human cell; leukemia cell; mitochondrial membrane potential; mitochondrion; photodynamic therapy; Aminolevulinic Acid; Animals; Apoptosis; Cell Line, Tumor; Drug Resistance, Neoplasm; Leukemia; Membrane Potential, Mitochondrial; Mice; Mitochondria; Photochemotherapy; Protoporphyrins; Reactive Oxygen Species; Superoxides; Ultraviolet Rays; Murinae |
| Año: | 2009 |
| Volumen: | 55 |
| Número: | 2 |
| Página de inicio: | 15 |
| Página de fin: | 19 |
| DOI: | http://dx.doi.org/10.1170/T847 |
| Título revista: | Cellular and Molecular Biology |
| Título revista abreviado: | Cell. Mol. Biol. |
| ISSN: | 01455680 |
| CODEN: | CMBID |
| CAS: | aminolevulinic acid, 106-60-5; dichlorodihydrofluorescein diacetate, 4091-99-0; doxorubicin, 23214-92-8, 25316-40-9; hydroethidine, 38483-26-0; protoporphyrin, 553-12-8; superoxide, 11062-77-4; vincristine, 57-22-7; Aminolevulinic Acid, 88755TAZ87; Protoporphyrins; Reactive Oxygen Species; Superoxides, 11062-77-4; protoporphyrin IX, 553-12-8 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01455680_v55_n2_p15_Diez |
Referencias:
- Bartosova, J., Hrkal, Z., Accumulation of protoporphyrin-IX (PpIX) in leukemic cell lines following induction by 5-aminolevulinic acid (ALA) (2000) Comp. Biochem. Physiol. C. Toxicol. Pharmacol., 126 (3), pp. 245-252
- Batlle, A.M.D.C., Porphyrins, Porphyrias, Cancer and Photodynamic Therapy - A model for carcinogenesis (1993) J. Photochem. Photobiol. B: Biol., 20, pp. 5-22
- Bernardi, P., Scorrano, L., Colonna, R., Petronilli, V., Di Lisa, F., Mitochondria and cell death (1999) Eur. J. Biochem., 264, pp. 687-701
- Buytaert, E., Dewaele, M., Agostinis, P., Molecular effectors of multiple cell death pathways initiated by photodynamic therapy (2007) Biochim. Biophys. Acta, 1776, pp. 86-107
- Capella, M.A., Capella, L.S., A light in multidrug resistance: Photodynamic treatment of multidrug-resistant tumors (2003) J. Biomed. Sci., 10 (4), pp. 361-366
- Fukuda, H., Casas, A., Batlle, A., Use of ALA and ALA derivatives for optimizing ALA based photodynamic therapy: A review of our experience (2006) J. Environ. Pathol. Toxicol. Oncol., 25, pp. 127-143
- Grebenova, D., Cajthamlova, H., Bartosova, J., Marinov, J., Klamova, H., Fuchs, O., Hrkal, Z., Selective destruction of leukaemic cells by photo-activation of 5- Aminolaevulinic acid-induced protoporphyrin-IX (1998) J. Photochem. Photobiol., 47, pp. 74-81
- Goldstein, B.D., Harber, L.C., Erythropoietic protoporphyria: Lipid peroxidation and red cell membrane damage associated with photohemolysis (1972) J. Clin. Invest., 51, pp. 892-899
- Kessel, D., Vicente, M.G.H., Reiners Jr., J.J., Initiation of apoptosis and autophagy by photodynamic therapy (2006) Autophagy, 2 (4), pp. 289-290
- Li, W., Zhang, W.J., Ohnishi, K., Yamada, I., Ohno, R., Hashimoto, K., 5-Aminolaevulinic acid-mediated photodynamic therapy in multidrug resistant leukemia cells (2001) J. Photochem. Photobiol. B: Biol., 60, pp. 79-86
- Merlin, J.L., Azzi, S., Lignon, D., Ramacci, C., Zeghari, N., Guillemin, F., MTT assays allow quick and reliable measurement of the response of human tumour cells to photodynamic therapy (1992) Eur. J. Cancer., 28, pp. 1452-1458
- Paz, M.L., González Maglio, D.H., Weill, F.S., Bustamante, J., Leoni, J., Mitochondrial dysfunction and cellular stress progression after ultraviolet B irradiation in human keratinocytes (2008) Photodermatol. Photoimmunol. Photomed., 24 (3), pp. 115-122
- Pushpan, S.K., Venkatraman, S., Anand, V.G., Sankar, J., Parmeswaran, D., Ganesan, S., Chandrashekar, T.K., Porphyrins in photodynamic therapy - A search for ideal photosensitizers (2002) Curr. Med. Chem. Anticancer Agents, 2 (2), pp. 187-207
- Sieber, F., Phototherapy, photochemotherapy, and bone marrow transplantation (1993) Journal of Hematotherapy, 2 (1), pp. 43-62
- Stuart, R.K., Autologous bone marrow transplantation for leukemia (1993) Semin. Oncol., 20 (6), pp. 40-54
Citas:
---------- APA ----------
Diez, B., Cordo Russo, R., Teijo, M.J., Hajos, S., Batlle, A. & Fukuda, H. (2009) . ROS production by endogenously generated protoporphyrin IX in murine leukemia cells. Cellular and Molecular Biology, 55(2), 15-19.http://dx.doi.org/10.1170/T847
---------- CHICAGO ----------
Diez, B., Cordo Russo, R., Teijo, M.J., Hajos, S., Batlle, A., Fukuda, H. "ROS production by endogenously generated protoporphyrin IX in murine leukemia cells" . Cellular and Molecular Biology 55, no. 2 (2009) : 15-19.http://dx.doi.org/10.1170/T847
---------- MLA ----------
Diez, B., Cordo Russo, R., Teijo, M.J., Hajos, S., Batlle, A., Fukuda, H. "ROS production by endogenously generated protoporphyrin IX in murine leukemia cells" . Cellular and Molecular Biology, vol. 55, no. 2, 2009, pp. 15-19.http://dx.doi.org/10.1170/T847
---------- VANCOUVER ----------
Diez, B., Cordo Russo, R., Teijo, M.J., Hajos, S., Batlle, A., Fukuda, H. ROS production by endogenously generated protoporphyrin IX in murine leukemia cells. Cell. Mol. Biol. 2009;55(2):15-19.http://dx.doi.org/10.1170/T847
