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Abstract:

Acute attacks of porphyria are most commonly precipitated by events that decrease heme concentrations. Enzyme inducing-drugs are the most important triggering factors, particularly in relation to anaesthesia. We have reported previously that Enflurane and Isoflurane produced significant heme metabolism alterations, indicating that the use of these anaesthetics in porphyric patients should be avoided. The aim of this work was to evaluate the effect of the anaesthetic Sevoflurane on heme pathway and drug metabolizing Phase I system in mice. To this end, animals received different doses of the anaesthetic (1-2 ml/kg) and were sacrificed at different times (5-60 min). Data revealed important alterations in the enzymes involved in Acute Intermittent Porphyria, such as an induction in hepatic 5-Aminolevulinic acid synthetase activity and a diminished Porphobilinogen deaminase activity in liver and blood 20 minutes after Sevoflurane administration to mice in a dose of 1.5 ml/kg. Heme oxygenase activity was also induced, indicating the onset of oxidative stress. Total CYP levels and CYP2E1 expression were enhanced. As a consequence of these events, heme free pool would be depleted. In conclusion, our results in mice would suggest that Sevoflurane should be used with caution and very careful control in porphyric patients. Copyright © 2009 C.M.B. Edition.

Registro:

Documento: Artículo
Título:Sevoflurane: Its action on heme metabolism and phase I drug metabolizing system
Autor:Sampayo, R.; Lavandera, J.V.; Batlle, A.; Buzaleh, A.M.
Filiación:Centro de Investigaciones Sobre Porfirinas Y Porfirias (CIPYP), Hospital de Clínicas José de San Martín, University of Buenos Aires, Argentina
Department of Biological Chemistry, Facultad de Ciencias Exactas Y Naturales, University of Buenos Aires, Argentina
México 1168 PB 19, C1097AAX - Buenos Aires, Argentina
Palabras clave:5-aminolevulinic acid synthetase; Cytochrome P450; Heme metabolism; PBG deaminase heme oxygenase; Sevoflurane; 5 aminolevulinate synthase; cytochrome P450 2E1; heme; heme oxygenase; porphobilinogen deaminase; sevoflurane; acute intermittent porphyria; animal experiment; animal tissue; article; controlled study; drug metabolism; enzyme activity; enzyme induction; male; mouse; nonhuman; oxidative stress; Western blotting; 5-Aminolevulinate Synthetase; Anesthetics, Inhalation; Animals; Cytochrome P-450 CYP2E1; Heme; Heme Oxygenase (Decyclizing); Hydroxymethylbilane Synthase; Liver; Male; Methyl Ethers; Mice; Oxidative Stress; Animalia; Mus
Año:2009
Volumen:55
Número:2
Página de inicio:140
Página de fin:146
DOI: http://dx.doi.org/10.1170/T863
Título revista:Cellular and Molecular Biology
Título revista abreviado:Cell. Mol. Biol.
ISSN:01455680
CODEN:CMBID
CAS:5 aminolevulinate synthase, 9037-14-3; heme, 14875-96-8; heme oxygenase, 9059-22-7; porphobilinogen deaminase, 9036-47-9, 9074-91-3; sevoflurane, 28523-86-6; 5-Aminolevulinate Synthetase, 2.3.1.37; Anesthetics, Inhalation; Cytochrome P-450 CYP2E1, 1.14.14.1; Heme, 42VZT0U6YR; Heme Oxygenase (Decyclizing), 1.14.99.3; Hydroxymethylbilane Synthase, 2.5.1.61; Methyl Ethers; sevoflurane, 28523-86-6
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01455680_v55_n2_p140_Sampayo

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Citas:

---------- APA ----------
Sampayo, R., Lavandera, J.V., Batlle, A. & Buzaleh, A.M. (2009) . Sevoflurane: Its action on heme metabolism and phase I drug metabolizing system. Cellular and Molecular Biology, 55(2), 140-146.
http://dx.doi.org/10.1170/T863
---------- CHICAGO ----------
Sampayo, R., Lavandera, J.V., Batlle, A., Buzaleh, A.M. "Sevoflurane: Its action on heme metabolism and phase I drug metabolizing system" . Cellular and Molecular Biology 55, no. 2 (2009) : 140-146.
http://dx.doi.org/10.1170/T863
---------- MLA ----------
Sampayo, R., Lavandera, J.V., Batlle, A., Buzaleh, A.M. "Sevoflurane: Its action on heme metabolism and phase I drug metabolizing system" . Cellular and Molecular Biology, vol. 55, no. 2, 2009, pp. 140-146.
http://dx.doi.org/10.1170/T863
---------- VANCOUVER ----------
Sampayo, R., Lavandera, J.V., Batlle, A., Buzaleh, A.M. Sevoflurane: Its action on heme metabolism and phase I drug metabolizing system. Cell. Mol. Biol. 2009;55(2):140-146.
http://dx.doi.org/10.1170/T863