Artículo

Martinez, M.D.C.; Afonso, S.G.; Meiss, R.P.; Buzaleh, A.M.; Batlle, A. "Hepatic damage and oxidative stress induced by griseofulvin in mice" (2009) Cellular and Molecular Biology. 55(2):127-139
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Abstract:

Erythropoietic Protoporphyria (EPP) is a disease associated with ferrochelatase deficiency, which produces accumulation of protoporphyrin IX (PROTO IX) in erythrocytes, liver and skin. In some cases, a severe hepatic failure and cholestasis was observed. Griseofulvin (Gris) develops an experimental EPP with hepatic manifestations in animals. The aim of this work was to further characterize this model studying its effect on different metabolisms in mice Gris feeding (0-2.5%, 7 and 14 days). PROTO IX accumulation in liver, blood and feces, induction of ALA-S activity, and a low rate of Holo/Apo tryptophan pyrrolase activity was produced, indicating a reduction of free heme pool. The progressive liver injury was reflected by the aspect and the enlargement of liver and the induction of hepatic damage. Liver redox balance was altered due to porphyrin high concentrations; as a consequence, the antioxidant defense system was disrupted. Heme oxygenase was also induced, however, at higher concentrations of antifungal, the free heme pool would be so depleted that this enzyme would not be necessary. In conclusion, our model of Protoporphyria produced liver alterations similar to those found in EPP patients. Copyright © 2009 C.M.B. Edition.

Registro:

Documento: Artículo
Título:Hepatic damage and oxidative stress induced by griseofulvin in mice
Autor:Martinez, M.D.C.; Afonso, S.G.; Meiss, R.P.; Buzaleh, A.M.; Batlle, A.
Filiación:Centro de Investigaciones Sobre Porfirinas Y Porfirias (CIPYP), CONICET, University of Buenos Aires, Argentina
Department of Biological Chemistry, Facultad de Ciencias Exactas Y Naturales, University of Buenos Aires, Argentina
Department of Pathology, Instituto de Estudios Oncológicos, Academia Nacional de Medicina, Buenos Aires, Argentina
Betharram 1720 - Martín Coronado, 1682, Buenos Aires, Argentina
Palabras clave:Erythropoietic Protoporphyria; Griseofulvin; Heme metabolism; Liver damage; Oxidative stress; 5 aminolevulinate synthase; alanine aminotransferase; alkaline phosphatase; aspartate aminotransferase; catalase; cytochrome P450 1A2; cytochrome P450 2A6; cytochrome P450 2C9; cytochrome P450 2E1; cytochrome P450 3A4; glutathione; glutathione peroxidase; glutathione reductase; glutathione transferase; griseofulvin; heme; heme oxygenase; malonaldehyde; protoporphyrin; reactive oxygen metabolite; superoxide dismutase; tryptophan 2,3 dioxygenase; alanine aminotransferase blood level; alkaline phosphatase blood level; animal experiment; animal model; animal tissue; antioxidant activity; article; aspartate aminotransferase blood level; blood level; body weight; controlled study; enzyme activity; enzyme induction; erythropoietic protoporphyria; experimental mouse; feces level; histopathology; liver injury; liver level; liver weight; male; mouse; nonhuman; oxidation reduction reaction; oxidative stress; Animals; Antifungal Agents; Aryl Hydrocarbon Hydroxylases; Cytochrome P-450 CYP3A; Disease Models, Animal; Griseofulvin; Heme; Heme Oxygenase (Decyclizing); Immunohistochemistry; Liver; Male; Mice; Oxidative Stress; Protoporphyria, Erythropoietic; Protoporphyrins; Tryptophan Oxygenase; Animalia; Mus
Año:2009
Volumen:55
Número:2
Página de inicio:127
Página de fin:139
DOI: http://dx.doi.org/10.1170/T862
Título revista:Cellular and Molecular Biology
Título revista abreviado:Cell. Mol. Biol.
ISSN:01455680
CODEN:CMBID
CAS:5 aminolevulinate synthase, 9037-14-3; alanine aminotransferase, 9000-86-6, 9014-30-6; alkaline phosphatase, 9001-78-9; aspartate aminotransferase, 9000-97-9; catalase, 9001-05-2; cytochrome P450 3A4, 329736-03-0; glutathione, 70-18-8; glutathione peroxidase, 9013-66-5; glutathione reductase, 9001-48-3; glutathione transferase, 50812-37-8; griseofulvin, 126-07-8; heme, 14875-96-8; heme oxygenase, 9059-22-7; malonaldehyde, 542-78-9; protoporphyrin, 553-12-8; superoxide dismutase, 37294-21-6, 9016-01-7, 9054-89-1; tryptophan 2,3 dioxygenase, 9014-51-1; Antifungal Agents; Aryl Hydrocarbon Hydroxylases, 1.14.14.1; CYP3A4 protein, mouse, 1.14.13.97; Cytochrome P-450 CYP3A, 1.14.14.1; Griseofulvin, 32HRV3E3D5; Heme, 42VZT0U6YR; Heme Oxygenase (Decyclizing), 1.14.99.3; Protoporphyrins; Tryptophan Oxygenase, 1.13.11.11; coumarin 7-hydroxylase, 1.14.14.1; protoporphyrin IX, 553-12-8
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01455680_v55_n2_p127_Martinez

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Citas:

---------- APA ----------
Martinez, M.D.C., Afonso, S.G., Meiss, R.P., Buzaleh, A.M. & Batlle, A. (2009) . Hepatic damage and oxidative stress induced by griseofulvin in mice. Cellular and Molecular Biology, 55(2), 127-139.
http://dx.doi.org/10.1170/T862
---------- CHICAGO ----------
Martinez, M.D.C., Afonso, S.G., Meiss, R.P., Buzaleh, A.M., Batlle, A. "Hepatic damage and oxidative stress induced by griseofulvin in mice" . Cellular and Molecular Biology 55, no. 2 (2009) : 127-139.
http://dx.doi.org/10.1170/T862
---------- MLA ----------
Martinez, M.D.C., Afonso, S.G., Meiss, R.P., Buzaleh, A.M., Batlle, A. "Hepatic damage and oxidative stress induced by griseofulvin in mice" . Cellular and Molecular Biology, vol. 55, no. 2, 2009, pp. 127-139.
http://dx.doi.org/10.1170/T862
---------- VANCOUVER ----------
Martinez, M.D.C., Afonso, S.G., Meiss, R.P., Buzaleh, A.M., Batlle, A. Hepatic damage and oxidative stress induced by griseofulvin in mice. Cell. Mol. Biol. 2009;55(2):127-139.
http://dx.doi.org/10.1170/T862