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Abstract:

Erythropoietic Protoporphyria (EPP) is an inherited deficiency of ferrochelatase, the last enzyme of the heme pathway. Under general anaesthesia, some patients develop neurological dysfunction suggesting upregulation in heme biosynthesis similar to that described for acute porphyrias after xenobiotic administration. Our aim has been to evaluate whether Isoflurane induces alterations in the heme pathway in a mouse model for EPP. Administration of Isoflurane (a single dose of 2 ml/kg, i.p) to wild-type (+/+), heterozygous (+/Fechm1Pas) and homozygous (Fechm1Pas/Fech m1Pas) mice, was evaluated by measuring the activity of δ-Aminolevulinic acid synthetase (ALA-S) and Porphobilinogen-deaminase (PBG-D) in different tissues, as well as Heme oxygenase (HO), cytochrome P-450, CYP2E1 and glutathione levels in liver. Porphyrin precursors were measured in 24h-urine samples. Fechm1Pas/Fechm1Pas mice receiving anaesthesia show enhanced ALA-S and CYP2E1 activities in the liver and increased urinary excretion of porphyrin precursors. No alterations were found in either PBG-D or HO activities. Diminished glutathione levels suggest that anaesthesia may produce oxidative stress in these animals. In conclusion, Isoflurane induces ALA-S activity and increased excretion of porphyrin precursors in EPP mice. These findings appear to confirm our previous hypothesis and indicate that Isoflurane may be an unsafe anaesthetic not only for patients with acute porphyrias but also for individuals with non acute porphyrias. Copyright © 2009 C.M.B. Edition.

Registro:

Documento: Artículo
Título:Induction of hepatic aminolevulinate acid synthetase activity by isoflurane in a genetic model for erythropoietic protoporphyria
Autor:Buzaleh, A.M.; Morán-Jiménez, M.J.; García-Bravo, M.; Sampedro, A.; Batlle, A.M.D.C.; Enríquez De Salamanca, R.; Fontanellas, A.
Filiación:Centro de Investigaciones Sobre Porfirinas Y Porfirias (CIPYP)-CONICET, University of Buenos Aires, Buenos Aires, Argentina
Department of Biological Chemistry, FCEN, University of Buenos Aires, Buenos Aires, Argentina
Porphyria Unit, Centro de Investigación, Hospital 12 de Octubre, Madrid, Spain
Gene Therapy and Hepatology Area, Centre for Applied Medical Research, University of Navarra, Pamplona, Spain
Francisco Gervás 9, 28020 Madrid, Spain
Palabras clave:Cytochrome P-450; Erythropoietic protoporphyria; Glutathione; Heme metabolism; Isoflurane; 5 aminolevulinate synthase; cytochrome P450; cytochrome P450 2E1; ferrochelatase; glutathione; heme oxygenase; isoflurane; porphobilinogen deaminase; porphyrin; animal experiment; animal model; animal tissue; article; controlled study; drug effect; drug mechanism; enzyme activity; erythropoietic protoporphyria; heme synthesis; male; mouse; nonhuman; signal transduction; urinary excretion; 5-Aminolevulinate Synthetase; Animals; Enzyme Activation; Enzyme Induction; Glutathione; Heme Oxygenase (Decyclizing); Hydroxymethylbilane Synthase; Isoflurane; Liver; Mice; Mice, Mutant Strains; Oxidative Stress; Protoporphyria, Erythropoietic; Animalia; Mus
Año:2009
Volumen:55
Número:1
Página de inicio:38
Página de fin:44
DOI: http://dx.doi.org/10.1170/T836
Título revista:Cellular and Molecular Biology
Título revista abreviado:Cell. Mol. Biol.
ISSN:01455680
CODEN:CMBID
CAS:5 aminolevulinate synthase, 9037-14-3; cytochrome P450, 9035-51-2; ferrochelatase, 9012-93-5; glutathione, 70-18-8; heme oxygenase, 9059-22-7; isoflurane, 26675-46-7; porphobilinogen deaminase, 9036-47-9, 9074-91-3; porphyrin, 24869-67-8; 5-Aminolevulinate Synthetase, 2.3.1.37; Glutathione, 70-18-8; Heme Oxygenase (Decyclizing), 1.14.99.3; Hydroxymethylbilane Synthase, 2.5.1.61; Isoflurane, 26675-46-7
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_01455680_v55_n1_p38_Buzaleh.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01455680_v55_n1_p38_Buzaleh

Referencias:

  • Afonso, S., Vanore, G., Batlle, A., Protoporphyrin IX and oxidative stress (1999) Free. Rad. Res., 31, pp. 161-170
  • Anderson, P.M., Desnick, R.J., Porphobilinogen deaminase: Methods and principles of the enzymatic assay (1982) Enzyme, 28 (2-3), pp. 146-156
  • Asokumar, B., Kierney, C., James, T.W., Amato, J., Tuman, K.J., Anaesthetic management of a patient with erythropoietic protoporphyria for ventricular septal defect closure (1999) Paediatric Anaesthesia, 9 (4), pp. 356-358. , DOI 10.1046/j.1460-9592.1999.00338.x
  • Berlin, A., Schaller, K.H., Grimes, H., Environmental exposure to lead: Analytical and epidemiological investigations using the European standardised method for blood delta aminolevulinic acid dehydratase activity determination (1977) International Archives of Occupational and Environmental Health, 39 (3), pp. 135-141. , DOI 10.1007/BF00405658
  • Bloomer, J.R., Rank, J.M., Payne, W.D., Shover, D.C., Sharp, H.L., Zwiener, R.J., Carithers, R.L., Follow-up after liver transplantation for protoporphyric liver disease (1996) Liver Transplantation and Surgery, 2 (4), pp. 269-275
  • Boulechfar, S., Lamoril, J., Montagutelli, X., Guenet, J.-L., Deybach -, J.C., Nordmann, Y., Dailey, H., De Verneuil, H., Ferrochelatase structural mutant (Fech(m1Pas)) in the house mouse (1993) Genomics, 16 (3), pp. 645-648. , DOI 10.1006/geno.1993.1242
  • Bradford, M.M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding (1976) Anal. Biochem., 72, pp. 248-254
  • Buzaleh, A.M., Del Carmen Batlle, A.M., Strain and sex differences in the effect of enflurane and isoflurane on heme metabolism in mice (1996) General Pharmacology, 27 (6), pp. 1009-1012. , DOI 10.1016/0306-3623(95)02137-X
  • Buzaleh, A.M., Enriquez De Salamanca, R., Del Carmen Batlle, A.M., Administration of the anesthetic isoflurane to mice: A model for acute intermittent porphyria? (1992) Journal of Pharmacological and Toxicological Methods, 28 (4), pp. 191-197
  • Buzaleh, A.M., Vazquez, E.S., Batlle Del, A.M.C., The involvement of microsomal drug metabolizing system during isoflurane anaesthesia in alcoholized animals (1996) Pharmacology Communications, 7 (2), pp. 101-106
  • Buzaleh, A.M., Vazquez, E., Enríquez De Salamanca, R., Batlle, A.M.D.C., An overview of anaesthesia and porphyria (1997) Endocrinol. Metab., 4, pp. 267-269. , Review
  • Buzaleh, A.M., Garcia-Bravo, M., Navarro, S., Moran-Jimenez, M.-J., Mendez, M., Batlle, A., Fontanellas, A., De Salamanca, R.E., Volatile anaesthetics induce biochemical alterations in the heme pathway in a B-lymphocyte cell line established from hepatoerythropoietic porphyria patients (LBHEP) and in mice inoculated with LBHEP cells (2004) International Journal of Biochemistry and Cell Biology, 36 (2), pp. 216-222. , DOI 10.1016/S1357-2725(03)00214-0
  • Davies, R., Schuurman, A., Barker, C.R., Clothier, B., Chernova, T., Higginson, F.M., Judah, D.J., Smith, A.G., Hepatic gene expression in protoporphyic Fech mice is associated with cholestatic injury but not a marked depletion of the heme regulatory pool (2005) American Journal of Pathology, 166 (4), pp. 1041-1053
  • Demasi, M., Penatti, C.A.A., Delucia, R., Bechara, E.J.H., The prooxidant effect of 5-aminolevulinic acid in the brain tissue of rats: Implications in neuropsychiatric manifestations in porphyrias (1996) Free Radical Biology and Medicine, 20 (3), pp. 291-299. , DOI 10.1016/0891-5849(95)02035-7
  • Eger II, E.I., Isoflurane: A review (1981) Anesthesiology, 55 (5), pp. 559-576
  • Fontanellas, A., Mazurier, F., Landry, M., Taine, L., Morel, C., Larou, M., Daniel, J.-Y., De Verneuil, H., Reversion of hepatobiliary alterations by bone marrow transplantation in a murine model of erythropoietic protoporphyria (2000) Hepatology, 32 (1), pp. 73-81
  • Gouya, L., Puy, H., Robreau, A.-M., Bourgeois, M., Lamoril, J., Da Silva, V., Grandchamp, B., Deybach, J.-C., The penetrance of dominant erythropoietic protoporphyria is modulated by expression of wildtype FECH (2002) Nature Genetics, 30 (1), pp. 27-28. , DOI 10.1038/ng809
  • Herbert, A., Corbin, D., Wiilliams, A., Thompson, D., Buckkels, J., Elias, E., Erythropoietic protoporphyria: Unusual skin and neurologic problems after liver transplantation (1991) Gastroenterology, 100, pp. 1753-1757
  • Jensen, N.F., Fiddler, D.S., Striepe, V., Anesthetic considerations in porphyrias (1995) Anesth. Analg., 80, pp. 591-599
  • Kharasch, E.D., Thummel, K.E., Identification of cytochrome P450 2E1 as the predominant enzyme catalyzing human liver microsomal defluorination of sevoflurane, isoflurane, and methoxyflurane (1993) Anesthesiology, 79 (4), pp. 795-807
  • Lieber, C.S., Cytochrome P-4502E1: Its physiological and pathological role (1997) Physiological Reviews, 77 (2), pp. 517-544
  • Lock, G., Holstege, A., Scholmerich, J., Neuhaus, P., Rank, J., Complications of transplantation for protoporphyric liver disease [5] (1994) Hepatology, 20 (6), pp. 1656-1657. , DOI 10.1002/hep.1840200652
  • Maines, M., Gibbs, P., 30 Some years of heme oxygenase: From a "molecular wrecking ball" to a "mesmerizing" trigger of cellular events (2005) Biochem. Biophys. Res. Commun., 338, pp. 568-577
  • Marver, H., Tschudy, D., Perlroth, M., Collins, A., Aminolevulinic acid synthetase I. Studies in liver homogenates (1966) J. Biol. Chem., 241, pp. 2803-2809
  • Navarro, S., Del Hoyo, P., Campos, Y., Abitbol, M., Moran-Jimenez, M.-J., Garcia-Bravo, M., Ochoa, P., Fontanellas, A., Increased mitochondrial respiratory chain enzyme activities correlate with minor extent of liver damage in mice suffering from erythropoietic protoporphyria (2005) Experimental Dermatology, 14 (1), pp. 26-33. , DOI 10.1111/j.0906-6705.2005.00248.x
  • Omura, T., Sato, R., The carbon monoxide-binding pigment of liver microsomes (1964) J. Biol. Chem., 239, pp. 2370-2378
  • Parera, V., Minderman, G., Koole, R., De Baar, E., Rossetti, M.V., Batlle, A., De Rooij, F., Protoporfiria Eritropoyética. Cinco nuevas mutaciones en el gen de la Ferroquelatasa (2000) Medicina, 60, p. 842
  • Quail, A.M., Modern inhalation anaesthetic agents. A review of halothane, isoflurane and enflurane (1987) Med. J. Austr., 150, pp. 95-102
  • Rank, J.M., Carithers, R., Bloomer, J., Evidence for neurological dysfunction in end-stage protoporphyric liver disease (1993) Hepatology, 18 (6), pp. 1404-1409. , DOI 10.1016/0270-9139(93)90231-B
  • Reinke, L.A., Moyer, M.J., A microsomal oxidation which is highly inducible by ethanol (1985) Drug Metabolism and Disposition, 13 (5), pp. 548-552
  • Rossi, R., Cardaioli, E., Scaloni, A., Amiconi, G., Di Simplicio, P., Thiol groups in proteins as endogenous reductans to determine glutathione protein mixed disulphides in biological systems (1995) Biochim. Biophys. Acta, 1243, pp. 230-238
  • Sarkany, R., Cox, T., Autosomal recessive erythropoietic protoporphyria: A syndrome of severe photosensitivity and liver failure (1995) Q J. Med., 88, pp. 541-549
  • Tenhunen, R., Marver, H.S., Schmid, R., The enzymatic catabolism of hemoglobin: Stimulation of microsomal heme oxygenase by hemin (1970) J Lab Clin Med, 75, pp. 410-421
  • Torrance, J.M., Anaesthetic management of erythropoietic protoporphyria (2000) Paediatr. Anaesth., 10, p. 571
  • Tutois, S., Montagutelli, X., Da Silva, V., Jouault, H., Rouyer-Fessard, P., Leroy-Viard, K., Guénet, J.L., Deybach, J.C., Erythropoietic protoporphyria in the house mouse. A recessive inherited ferrochelatase deficiency with anemia, photosensitivity, and liver disease (1991) J. Clin. Invest., 88, pp. 1730-1736

Citas:

---------- APA ----------
Buzaleh, A.M., Morán-Jiménez, M.J., García-Bravo, M., Sampedro, A., Batlle, A.M.D.C., Enríquez De Salamanca, R. & Fontanellas, A. (2009) . Induction of hepatic aminolevulinate acid synthetase activity by isoflurane in a genetic model for erythropoietic protoporphyria. Cellular and Molecular Biology, 55(1), 38-44.
http://dx.doi.org/10.1170/T836
---------- CHICAGO ----------
Buzaleh, A.M., Morán-Jiménez, M.J., García-Bravo, M., Sampedro, A., Batlle, A.M.D.C., Enríquez De Salamanca, R., et al. "Induction of hepatic aminolevulinate acid synthetase activity by isoflurane in a genetic model for erythropoietic protoporphyria" . Cellular and Molecular Biology 55, no. 1 (2009) : 38-44.
http://dx.doi.org/10.1170/T836
---------- MLA ----------
Buzaleh, A.M., Morán-Jiménez, M.J., García-Bravo, M., Sampedro, A., Batlle, A.M.D.C., Enríquez De Salamanca, R., et al. "Induction of hepatic aminolevulinate acid synthetase activity by isoflurane in a genetic model for erythropoietic protoporphyria" . Cellular and Molecular Biology, vol. 55, no. 1, 2009, pp. 38-44.
http://dx.doi.org/10.1170/T836
---------- VANCOUVER ----------
Buzaleh, A.M., Morán-Jiménez, M.J., García-Bravo, M., Sampedro, A., Batlle, A.M.D.C., Enríquez De Salamanca, R., et al. Induction of hepatic aminolevulinate acid synthetase activity by isoflurane in a genetic model for erythropoietic protoporphyria. Cell. Mol. Biol. 2009;55(1):38-44.
http://dx.doi.org/10.1170/T836