The effect of sucrose on tuber formation, calcium-dependent protein kinase (CDPK) and phosphatase activities was analysed using in vitro cultured potato plants. In short treatments, sucrose induced CDPK and phosphatase activities. In long treatments, sucrose induced tuber formation in the absence of other tuber inducing stimuli. Sorbitol caused a minor increase in CDPK activity and affected plant morphology but did not induce tuber development. The addition of the protein kinase inhibitor Staurosporine precluded sucrose-induced tuberization. Altogether, our results suggest that phosphorylation/dephosphorylation events are involved in sucrose-induced tuber development.
Documento: | Artículo |
Título: | Sucrose increases calcium-dependent protein kinase and phosphatase activities in potato plants. |
Autor: | Raíces, M.; MacIntosh, G.C.; Ulloa, R.M.; Gargantini, P.R.; Vozza, N.F.; Téllez-Inón, M.T. |
Filiación: | Instituto de Investigaciones en Ingeniería Genética y Biología Molecular and Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Vuelta de Obligado 2490, 1428 Buenos Aires, Argentina |
Palabras clave: | calcium; calcium protein kinase; calcium-dependent protein kinase; phosphatase; protein kinase; sucrose; article; enzymology; growth, development and aging; metabolism; potato; Calcium; Phosphoric Monoester Hydrolases; Protein Kinases; Solanum tuberosum; Sucrose |
Año: | 2003 |
Volumen: | 49 |
Número: | 6 |
Página de inicio: | 959 |
Página de fin: | 964 |
Título revista: | Cellular and molecular biology (Noisy-le-Grand, France) |
Título revista abreviado: | Cell. Mol. Biol. (Noisy-le-grand) |
ISSN: | 01455680 |
CAS: | calcium, 7440-70-2, 14092-94-5; phosphatase, 9013-05-2; protein kinase, 9026-43-1; sucrose, 122880-25-5, 57-50-1; Calcium, 7440-70-2; calcium-dependent protein kinase, EC 2.7.1.-; Phosphoric Monoester Hydrolases, EC 3.1.3.-, 7440-70-2; Protein Kinases, EC 2.7.1.37, EC 3.1.3.-; Sucrose, 57-50-1 |
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01455680_v49_n6_p959_Raices |