In the yeast Saccharomyces cerevisiae, gamma-aminobutyric acid (GABA) transport is mediated by three permeases: the general amino acid permease GAP1, the proline permease PUT4 and the specific GABA permease UGA4. Expression of UGA4 gene is induced in the presence of GABA. We started a comparative study about UGA4 gene induction in cells grown on different culture media. Results presented here indicate that under certain growth conditions UGA4 permease is constitutive. Therefore, we demonstrate that in S. cerevisiae the UGA4 gene expression depends on cell growth conditions and that its synthesis not always depends on the presence of GABA.
Documento: | Artículo |
Título: | UGA4 gene expression in Saccharomyces cerevisiae depends on cell growth conditions. |
Autor: | Bermúdez Moretti, M.; Correa García, S.; Batlle, A. |
Filiación: | Centro de Investigaciones sobre Porfirinas y Porfirias, (CONCIET, CIPYP, FCEyN, UBA), Pabellón II, Ciudad Universitaria, Buenos Aires, Argentina |
Palabras clave: | 4 aminobutyric acid; 4 aminobutyric acid carrier; amino acid transporter; carrier protein; GABA permease; organic anion transporter; proline; proline transporter; Saccharomyces cerevisiae protein; UGA4 protein, S cerevisiae; article; cell division; comparative study; culture medium; drug effect; enzymology; gene expression regulation; genetics; growth, development and aging; metabolism; mutation; pH; Saccharomyces cerevisiae; Amino Acid Transport Systems, Neutral; Cell Division; Culture Media; GABA Plasma Membrane Transport Proteins; gamma-Aminobutyric Acid; Gene Expression Regulation, Fungal; Hydrogen-Ion Concentration; Membrane Transport Proteins; Mutation; Organic Anion Transporters; Proline; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins |
Año: | 1998 |
Volumen: | 44 |
Número: | 4 |
Página de inicio: | 585 |
Página de fin: | 590 |
Título revista: | Cellular and molecular biology (Noisy-le-Grand, France) |
Título revista abreviado: | Cell. Mol. Biol. (Noisy-le-grand) |
ISSN: | 01455680 |
CAS: | 4 aminobutyric acid, 28805-76-7, 56-12-2; carrier protein, 80700-39-6; proline, 147-85-3, 7005-20-1; Amino Acid Transport Systems, Neutral; Culture Media; GABA permease, 69913-01-5, 9040-98-6; GABA Plasma Membrane Transport Proteins; gamma-Aminobutyric Acid, 56-12-2; Membrane Transport Proteins; Organic Anion Transporters; Proline, 147-85-3; proline transporter, 9040-98-6; Saccharomyces cerevisiae Proteins; UGA4 protein, S cerevisiae |
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01455680_v44_n4_p585_BermudezMoretti |