Artículo

Parussini, F.; Duschak, V.G.; Cazzulo, J.J. "Membrane-bound cysteine proteinase isoforms in different developmental stages of Trypanosoma cruzi." (1998) Cellular and molecular biology (Noisy-le-Grand, France). 44(3):513-519
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Abstract:

Cysteine proteinase isoforms, immunologically cross-reactive with cruzipain and with a similar apparent molecular mass, have been identified in epimastigotes of Trypanosoma cruzi by extraction and phase partition using the detergent Triton X-114. These isoforms are concentrated in the microsomal fraction obtained after differential centrifugation, which is known to consist essentially of plasma membrane, can be labelled by incubation of live parasites with sulfo-NHS-biotin, and bind to cystatin-sepharose affinity columns. They are present, albeit with a different electrophoretic pattern, in the epimastigote, amastigote and trypomastigote stages of the parasite.

Registro:

Documento: Artículo
Título:Membrane-bound cysteine proteinase isoforms in different developmental stages of Trypanosoma cruzi.
Autor:Parussini, F.; Duschak, V.G.; Cazzulo, J.J.
Filiación:Instituto de Investigaciones Bioquímicas Luis F. Leloir, Fundación Campomar, CONICET, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina
Palabras clave:cysteine proteinase; isoenzyme; animal; article; cell membrane; enzymology; metabolism; Trypanosoma cruzi; Animals; Cell Membrane; Cysteine Endopeptidases; Isoenzymes; Trypanosoma cruzi
Año:1998
Volumen:44
Número:3
Página de inicio:513
Página de fin:519
Handle:http://hdl.handle.net/20.500.12110/paper_01455680_v44_n3_p513_Parussini
Título revista:Cellular and molecular biology (Noisy-le-Grand, France)
Título revista abreviado:Cell. Mol. Biol. (Noisy-le-grand)
ISSN:01455680
CAS:cysteine proteinase, 37353-41-6; mucosa associated lymphoid tissue lymphoma translocation protein 1; Cysteine Endopeptidases, EC 3.4.22.-; Isoenzymes
PDF:https://bibliotecadigital.exactas.uba.ar/download/paper/paper_01455680_v44_n3_p513_Parussini.pdf
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01455680_v44_n3_p513_Parussini

Citas:

---------- APA ----------
Parussini, F., Duschak, V.G. & Cazzulo, J.J. (1998) . Membrane-bound cysteine proteinase isoforms in different developmental stages of Trypanosoma cruzi. Cellular and molecular biology (Noisy-le-Grand, France), 44(3), 513-519.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01455680_v44_n3_p513_Parussini [ ]
---------- CHICAGO ----------
Parussini, F., Duschak, V.G., Cazzulo, J.J. "Membrane-bound cysteine proteinase isoforms in different developmental stages of Trypanosoma cruzi." Cellular and molecular biology (Noisy-le-Grand, France) 44, no. 3 (1998) : 513-519.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01455680_v44_n3_p513_Parussini [ ]
---------- MLA ----------
Parussini, F., Duschak, V.G., Cazzulo, J.J. "Membrane-bound cysteine proteinase isoforms in different developmental stages of Trypanosoma cruzi." Cellular and molecular biology (Noisy-le-Grand, France), vol. 44, no. 3, 1998, pp. 513-519.
Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01455680_v44_n3_p513_Parussini [ ]
---------- VANCOUVER ----------
Parussini, F., Duschak, V.G., Cazzulo, J.J. Membrane-bound cysteine proteinase isoforms in different developmental stages of Trypanosoma cruzi. Cell. Mol. Biol. (Noisy-le-grand). 1998;44(3):513-519.
Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01455680_v44_n3_p513_Parussini [ ]