Rat brain glycogen branching enzyme was partially purified in order to elucidate its mechanism of action. The alpha1,4-alpha1,6-glucan polysaccharide was synthesized using rat brain branching enzyme under two different elongation conditions: Glc-1-P and phosphorylase or UDP-Glc and glycogen synthase. The products obtained demonstrated that the cpolysaccharides synthesized (pattern of the spectra obtained in the presence of Krisman's reagent, lambda max, parameter A and R, % beta-amylolysis and degree of branching) under different incubation times are nearly constant. These results imply that the degree of branching of a polysaccharide depends only on the enzyme specificity.
Documento: | Artículo |
Título: | Glycogen brain branching enzyme. |
Autor: | Tolmasky, D.S.; Labriola, C.; Krisman, C.R. |
Filiación: | Instituto de Investigaciones Bioquímicas Luis F. Leloir Fundación Campomar, Consejo Nacional de Investigaciones Científicas y Técnicas, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina |
Palabras clave: | 1,4 alpha glucan branching enzyme; beta amylase; glucose 1 phosphate; glucose phosphate; phosphorylase; polysaccharide; absorption; animal; article; biosynthesis; brain; enzymology; metabolism; rat; Wistar rat; 1,4-alpha-Glucan Branching Enzyme; Absorption; Animals; beta-Amylase; Brain; Glucosephosphates; Phosphorylases; Polysaccharides; Rats; Rats, Wistar |
Año: | 1998 |
Volumen: | 44 |
Número: | 3 |
Página de inicio: | 455 |
Página de fin: | 460 |
Título revista: | Cellular and molecular biology (Noisy-le-Grand, France) |
Título revista abreviado: | Cell. Mol. Biol. (Noisy-le-grand) |
ISSN: | 01455680 |
CAS: | 1,4 alpha glucan branching enzyme, 9001-97-2; beta amylase, 9000-91-3; glucose 1 phosphate, 59-56-3; phosphorylase, 9035-74-9; 1,4-alpha-Glucan Branching Enzyme, EC 2.4.1.18; beta-Amylase, EC 3.2.1.2, EC 2.4.1.18; glucose-1-phosphate, 59-56-3, EC 2.4.1.-; Glucosephosphates; Phosphorylases, EC 2.4.1.-; Polysaccharides |
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01455680_v44_n3_p455_Tolmasky |