Artículo
Nastri, H.G.; Algranati, I.D. "Effect of polyamines on plasmid-mediated kanamycin resistance and kanamycin phosphotransferase gene expression in Escherichia coli." (1996) Cellular and molecular biology (Noisy-le-Grand, France). 42(5):711-717
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Abstract:
The emergence of kanamycin resistance in a polyamine-deficient mutant of E. coli transformed with a plasmid encoding the kanamycin phosphotransferase gene has been studied. The initial inhibition of growth and protein synthesis caused by the addition of the antibiotic could be reversed earlier in polyamine-supplemented bacteria than in those depleted of the organic bases. Concomitantly, we have observed that the increase of kanamycin phosphotransferase activity evoking the antibiotic resistance was higher in bacteria cultivated in the presence of putrescine. This result seems to depend exclusively on the enhanced capacity of the translation process in bacteria grown with polyamines since the transcription of phosphotransferase gene was higher in cells subjected to polyamine starvation.
Registro:
| Documento: | Artículo |
| Título: | Effect of polyamines on plasmid-mediated kanamycin resistance and kanamycin phosphotransferase gene expression in Escherichia coli. |
| Autor: | Nastri, H.G.; Algranati, I.D. |
| Filiación: | Instituto de Investigaciones Bioquímicas Fundación Campomar, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina |
| Palabras clave: | bacterial protein; bacterial RNA; kanamycin kinase; messenger RNA; phosphotransferase; polyamine; putrescine; antibiotic resistance; article; bacterial gene; biosynthesis; drug effect; enzymology; Escherichia coli; gene expression; genetic transformation; genetics; kinetics; metabolism; plasmid; Bacterial Proteins; Escherichia coli; Gene Expression; Genes, Bacterial; Kanamycin Kinase; Kanamycin Resistance; Kinetics; Phosphotransferases (Alcohol Group Acceptor); Plasmids; Polyamines; Putrescine; RNA, Bacterial; RNA, Messenger; Transformation, Genetic |
| Año: | 1996 |
| Volumen: | 42 |
| Número: | 5 |
| Página de inicio: | 711 |
| Página de fin: | 717 |
| Título revista: | Cellular and molecular biology (Noisy-le-Grand, France) |
| Título revista abreviado: | Cell. Mol. Biol. (Noisy-le-grand) |
| ISSN: | 01455680 |
| CAS: | kanamycin kinase, 62213-36-9; phosphotransferase, 9031-09-8, 9031-44-1; putrescine, 110-60-1, 333-93-7; Bacterial Proteins; Kanamycin Kinase, EC 2.7.1.95, 110-60-1; Phosphotransferases (Alcohol Group Acceptor), EC 2.7.1.-; Polyamines; Putrescine, 110-60-1; RNA, Bacterial; RNA, Messenger |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01455680_v42_n5_p711_Nastri |
Citas:
---------- APA ----------
Nastri, H.G. & Algranati, I.D. (1996) . Effect of polyamines on plasmid-mediated kanamycin resistance and kanamycin phosphotransferase gene expression in Escherichia coli. Cellular and molecular biology (Noisy-le-Grand, France), 42(5), 711-717.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01455680_v42_n5_p711_Nastri [ ]
---------- CHICAGO ----------
Nastri, H.G., Algranati, I.D. "Effect of polyamines on plasmid-mediated kanamycin resistance and kanamycin phosphotransferase gene expression in Escherichia coli." Cellular and molecular biology (Noisy-le-Grand, France) 42, no. 5 (1996) : 711-717.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01455680_v42_n5_p711_Nastri [ ]
---------- MLA ----------
Nastri, H.G., Algranati, I.D. "Effect of polyamines on plasmid-mediated kanamycin resistance and kanamycin phosphotransferase gene expression in Escherichia coli." Cellular and molecular biology (Noisy-le-Grand, France), vol. 42, no. 5, 1996, pp. 711-717.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01455680_v42_n5_p711_Nastri [ ]
---------- VANCOUVER ----------
Nastri, H.G., Algranati, I.D. Effect of polyamines on plasmid-mediated kanamycin resistance and kanamycin phosphotransferase gene expression in Escherichia coli. Cell. Mol. Biol. (Noisy-le-grand). 1996;42(5):711-717.Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01455680_v42_n5_p711_Nastri [ ]
