Artículo
Abstract:
UDP-glucose: protein transglucosylase (UPTG, EC 2.4.1.112) catalyzes the first step of protein-bound alpha-glucan synthesis in potato tuber and developing maize endosperm. The presence of a non-dialyzable, heat labile protein responsible for low levels of UPTG activity in developing maize endosperm was investigated. UPTG activity in 5-day old maize seedlings and potato tuber solubilized preparations was also reduced by the endosperm preparation. FPLC-Mono Q column chromatography of developing maize endosperm was effective in separating the inhibitor protein (IP) from UPTG. After gel filtration on Superose 12, IP yielded a major polypeptide of about 80 kDa on SDS-PAGE. IP was purified by gel filtration on Superose 12 and preparative SDS-PAGE, and specific antibodies were prepared. Polyclonal antibodies reacted specifically with an 80 kDa polypeptide of developing maize endosperm on Western blot. They also recognized a similar band in 5-day old maize seedlings, but not in potato tubers. The identification of a factor that regulates the level of UPTG activity in developing maize endosperm may help to elucidate the functional role of the enzyme in the initiation of starch synthesis during seed development.
Registro:
| Documento: | Artículo |
| Título: | Inhibition of UDP-glucose: protein transglucosylase by a maize endosperm protein factor. |
| Autor: | Rothschild, A.; Wald, F.A.; Bocca, S.N.; Tandecarz, J.S. |
| Filiación: | Instituto de Investigaciones Bioquímicas Fundación Campomar, I.I.B.B.A., CONICET, Facultad de Ciencias Exactas y Naturales, U.B.A., Argentina. |
| Palabras clave: | alpha 1,4 glucan protein synthase (UDP forming); alpha-1,4-glucan-protein synthase (UDP-forming); enzyme inhibitor; glucosyltransferase; starch; vegetable protein; article; biosynthesis; drug antagonism; enzymology; growth, development and aging; isolation and purification; maize; metabolism; molecular weight; solubility; Enzyme Inhibitors; Glucosyltransferases; Molecular Weight; Plant Proteins; Solubility; Starch; Zea mays |
| Año: | 1996 |
| Volumen: | 42 |
| Número: | 5 |
| Página de inicio: | 645 |
| Página de fin: | 651 |
| Título revista: | Cellular and molecular biology (Noisy-le-Grand, France) |
| Título revista abreviado: | Cell. Mol. Biol. (Noisy-le-grand) |
| ISSN: | 01455680 |
| CAS: | alpha-1,4-glucan-protein synthase (UDP-forming), EC 2.4.1.112; Enzyme Inhibitors; Glucosyltransferases, EC 2.4.1.-; Plant Proteins; Starch, 9005-25-8 |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01455680_v42_n5_p645_Rothschild |
Citas:
---------- APA ----------
Rothschild, A., Wald, F.A., Bocca, S.N. & Tandecarz, J.S. (1996) . Inhibition of UDP-glucose: protein transglucosylase by a maize endosperm protein factor. Cellular and molecular biology (Noisy-le-Grand, France), 42(5), 645-651.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01455680_v42_n5_p645_Rothschild [ ]
---------- CHICAGO ----------
Rothschild, A., Wald, F.A., Bocca, S.N., Tandecarz, J.S. "Inhibition of UDP-glucose: protein transglucosylase by a maize endosperm protein factor." Cellular and molecular biology (Noisy-le-Grand, France) 42, no. 5 (1996) : 645-651.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01455680_v42_n5_p645_Rothschild [ ]
---------- MLA ----------
Rothschild, A., Wald, F.A., Bocca, S.N., Tandecarz, J.S. "Inhibition of UDP-glucose: protein transglucosylase by a maize endosperm protein factor." Cellular and molecular biology (Noisy-le-Grand, France), vol. 42, no. 5, 1996, pp. 645-651.Recuperado de https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01455680_v42_n5_p645_Rothschild [ ]
---------- VANCOUVER ----------
Rothschild, A., Wald, F.A., Bocca, S.N., Tandecarz, J.S. Inhibition of UDP-glucose: protein transglucosylase by a maize endosperm protein factor. Cell. Mol. Biol. (Noisy-le-grand). 1996;42(5):645-651.Available from: https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01455680_v42_n5_p645_Rothschild [ ]
