Three wild type strains of Rhizobium fredii, USDA 191, USDA 257 and HH 303, do not synthesize in vivo or in vitro beta(1-3), beta(1-6) cyclic glucans, all strains form in vitro and in vivo cyclic beta(1-2) glucans. Approximately 80% of the recovered R. fredii cellular cyclic beta(1-2) glucans were anionic and the substituent was identified as phosphoglycerol. Inner membranes prepared from these R. fredii strains have a beta(1-2) glucan-intermediate-protein with apparent molecular mass undistinguishable from Agrobacterium tumefaciens beta(1-2) glucan intermediate protein. Studies of the degree of polymerization of the oligosaccharides recovered from the protein-intermediate after short pulse incubations with UDP-14C-glucose suggested that the rate limiting step in the biosynthesis of cyclic glucan is cyclization. Kinetic studies revealed that the K(m) for UDP-glucose was 0.33 mM. No difference was detected between the K(m) for initiation/elongation and cyclization reactions. Nodulation studies of a ndvB R. fredii mutant with Mc Call and Peking soybean cultivars, revealed that beta(1-2) glucans do not seem to be required for normal nodule invasion of these soybean cultivars.
| Documento: | Artículo |
| Título: | Characterization of the biosynthesis of beta(1-2) cyclic glucan in R. Fredii. Beta(1-2) glucan has no apparent role in nodule invasion of Mc Call and Peking soybean cultivars. |
| Autor: | Iñón de Iannino, N.; Briones, G.; Kreiman, G.; Ugalde, R. |
| Filiación: | ñó Facultad de Ciencias Exactas y Naturales, Instituto de Investigaciones Bioquímicas, CONICET, Universidad de Buenos Aires, Argentina |
| Palabras clave: | bacterial protein; beta glucan; cyclic beta(1 2)glucan; cyclic beta(1-2)glucan; glucan; glycopeptide; membrane protein; uridine diphosphate glucose; article; biosynthesis; chemical structure; chemistry; classification; comparative study; electron microscopy; isolation and purification; kinetics; metabolism; microbiology; molecular weight; Rhizobium; soybean; species difference; symbiosis; ultrastructure; Bacterial Proteins; beta-Glucans; Glucans; Glycopeptides; Kinetics; Membrane Proteins; Microscopy, Electron; Molecular Structure; Molecular Weight; Rhizobium; Soybeans; Species Specificity; Symbiosis; Uridine Diphosphate Glucose |
| Año: | 1996 |
| Volumen: | 42 |
| Número: | 5 |
| Página de inicio: | 617 |
| Página de fin: | 629 |
| Título revista: | Cellular and molecular biology (Noisy-le-Grand, France) |
| Título revista abreviado: | Cell. Mol. Biol. (Noisy-le-grand) |
| ISSN: | 01455680 |
| CAS: | glucan, 9012-72-0, 9037-91-6; uridine diphosphate glucose, 133-89-1; Bacterial Proteins; beta-Glucans; cyclic beta(1-2)glucan, 116243-01-7; Glucans; Glycopeptides; Membrane Proteins; Uridine Diphosphate Glucose, 133-89-1 |
| PDF: | https://bibliotecadigital.exactas.uba.ar/download/paper/paper_01455680_v42_n5_p617_InondeIannino.pdf |
| Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01455680_v42_n5_p617_InondeIannino |
