The aim of this work was to characterize the 4-aminobutyric acid (GABA) transport in the Saccharomyces cerevisiae D27 strain, followed by the study of the relationship between 5-aminolevulinic acid (ALA) and GABA transport systems. It was found that the general amino acid permease (GAP) is not active in D27 strain, suggesting that GABA incorporation should be mediated by PUT4 and UGA4 permeases. However, after kinetic studies only one system was detected. It was also shown that GABA uptake is competitively inhibited by ALA. GABA incorporation is regulated by the carbon source but not by the nitrogen source. When cells were grown in the presence of GABA, its entrance was very low.
Documento: | Artículo |
Título: | GABA uptake in a Saccharomyces cerevisiae strain. |
Autor: | Bermúdez Moretti, M.; Correa García, S.; Ramos, E.H.; Batlle, A. |
Filiación: | Centro de Investigaciones sobre Porfirinas y Porfirias, CIPYP (CONICET, FCEyN, UBA), Ciudad Universitaria, Buenos Aires, Argentina |
Palabras clave: | 4 aminobutyric acid; 4 aminobutyric acid carrier; amino acid; amino acid transporter; aminolevulinic acid; carrier protein; GABA permease; organic anion transporter; proline transporter; Saccharomyces cerevisiae protein; UGA4 protein, S cerevisiae; article; comparative study; drug effect; growth, development and aging; kinetics; metabolism; Saccharomyces cerevisiae; species difference; transport at the cellular level; Amino Acid Transport Systems; Amino Acid Transport Systems, Neutral; Amino Acids; Aminolevulinic Acid; Biological Transport; GABA Plasma Membrane Transport Proteins; gamma-Aminobutyric Acid; Kinetics; Membrane Transport Proteins; Organic Anion Transporters; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; Species Specificity |
Año: | 1995 |
Volumen: | 41 |
Número: | 6 |
Página de inicio: | 843 |
Página de fin: | 849 |
Título revista: | Cellular and molecular biology (Noisy-le-Grand, France) |
Título revista abreviado: | Cell. Mol. Biol. (Noisy-le-grand) |
ISSN: | 01455680 |
CAS: | 4 aminobutyric acid, 28805-76-7, 56-12-2; amino acid, 65072-01-7; aminolevulinic acid, 106-60-5; carrier protein, 80700-39-6; Amino Acid Transport Systems; Amino Acid Transport Systems, Neutral; Amino Acids; Aminolevulinic Acid, 106-60-5; GABA permease, 69913-01-5, 56-12-2; GABA Plasma Membrane Transport Proteins; gamma-Aminobutyric Acid, 56-12-2; Membrane Transport Proteins; Organic Anion Transporters; proline transporter, 9040-98-6; Saccharomyces cerevisiae Proteins; UGA4 protein, S cerevisiae |
Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01455680_v41_n6_p843_BermudezMoretti |