Artículo

Chimeo, C.; Fernandez-Gimenez, A.V.; Campanella, M.; Mendez-Romero, O.; Muhlia-Almazan, A. "The shrimp mitochondrial FoF1-ATPase inhibitory factor 1 (IF1)" (2015) Journal of Bioenergetics and Biomembranes. 47(5):383-393
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Abstract:

The whiteleg shrimp species Litopenaeus vannamei is exposed to cyclic changes of the dissolved oxygen concentration of seawater and must neutralize the adverse effects of hypoxia by using ATP as energy source. In crustaceans, the mitochondrial FOF1-ATP synthase is pivotal to the homeostasis of ATP and function prevalently as a FOF1-ATPase. Hitherto, it is unknown whether these marine invertebrates are equipped with molecules able to control the FOF1-ATPase inhibiting the ATP consumption. In this study, we report two variants of the mitochondrial FOF1-ATPase Inhibitory Factor 1 (IF1) ubiquitously expressed across tissues of the Litopenaeus vannamei transcriptome: the IF1_Lv1 and the IF1_Lv2. The IF1_Lv1, with a full-length sequence of 550 bp, encodes a 104 aa long protein and its mRNA amounts are significantly affected by hypoxia and re-oxygenation. The IF1_Lv2, with a sequence of 654 bp, encodes instead for a protein of 85 aa. Both proteins share a 69 % homology and contain a conserved minimal inhibitory sequence (IATP domain) along with a G-rich region on their N-terminus typical of the invertebrate. In light of this characterization IF1 is here discussed as an adaptive mechanism evolved by this marine species to inhibit the FOF1-ATPase activity and avoid ATP dissipation to thrive in spite of the changes in oxygen tension. © 2015, Springer Science+Business Media New York.

Registro:

Documento: Artículo
Título:The shrimp mitochondrial FoF1-ATPase inhibitory factor 1 (IF1)
Autor:Chimeo, C.; Fernandez-Gimenez, A.V.; Campanella, M.; Mendez-Romero, O.; Muhlia-Almazan, A.
Filiación:Bioenergetics and Molecular Genetics Lab, Centro de Investigacion en Alimentacion y Desarrollo (CIAD) A. C, Carretera a Ejido La Victoria Km 0.6, PO Box 1735, Hermosillo, Sonora, Mexico
Laboratorio Fisiologia de Organismos Acuaticos. Instituto de Investigaciones Marinas y Costeras, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, Buenos Aires, Argentina
Department of Comparative Biomedical Sciences, The Royal Veterinary College, University of London, London, United Kingdom
University College London Consortium for Mitochondrial Research, Royal College Street NW1 0TU, London, United Kingdom
Palabras clave:Hypoxia; IF1, FoF1-ATPase; Inhibitor; Shrimp; Transcriptional regulation; adenosine triphosphatase; complementary DNA; fibrinogen; inhibitory factor 1; messenger RNA; transcriptome; unclassified drug; arthropod protein; ATPase inhibitory protein; protein; proton transporting adenosine triphosphatase; adult; amino acid sequence; animal tissue; Article; controlled study; DNA sequence; enzyme activity; gene expression; gene sequence; genetic variability; Litopenaeus vannamei; marine species; mitochondrion; nonhuman; nucleotide sequence; oxygen tension; phylogeny; reoxygenation; RNA sequence; sequence homology; animal; genetics; metabolism; molecular genetics; Penaeidae; Animals; Arthropod Proteins; Base Sequence; Molecular Sequence Data; Penaeidae; Proteins; Proton-Translocating ATPases
Año:2015
Volumen:47
Número:5
Página de inicio:383
Página de fin:393
DOI: http://dx.doi.org/10.1007/s10863-015-9621-0
Título revista:Journal of Bioenergetics and Biomembranes
Título revista abreviado:J. Bioenerg. Biomembr.
ISSN:0145479X
CODEN:JBBID
CAS:adenosine triphosphatase, 37289-25-1, 9000-83-3; fibrinogen, 9001-32-5; protein, 67254-75-5; proton transporting adenosine triphosphatase; Arthropod Proteins; ATPase inhibitory protein; Proteins; Proton-Translocating ATPases
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_0145479X_v47_n5_p383_Chimeo

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Citas:

---------- APA ----------
Chimeo, C., Fernandez-Gimenez, A.V., Campanella, M., Mendez-Romero, O. & Muhlia-Almazan, A. (2015) . The shrimp mitochondrial FoF1-ATPase inhibitory factor 1 (IF1). Journal of Bioenergetics and Biomembranes, 47(5), 383-393.
http://dx.doi.org/10.1007/s10863-015-9621-0
---------- CHICAGO ----------
Chimeo, C., Fernandez-Gimenez, A.V., Campanella, M., Mendez-Romero, O., Muhlia-Almazan, A. "The shrimp mitochondrial FoF1-ATPase inhibitory factor 1 (IF1)" . Journal of Bioenergetics and Biomembranes 47, no. 5 (2015) : 383-393.
http://dx.doi.org/10.1007/s10863-015-9621-0
---------- MLA ----------
Chimeo, C., Fernandez-Gimenez, A.V., Campanella, M., Mendez-Romero, O., Muhlia-Almazan, A. "The shrimp mitochondrial FoF1-ATPase inhibitory factor 1 (IF1)" . Journal of Bioenergetics and Biomembranes, vol. 47, no. 5, 2015, pp. 383-393.
http://dx.doi.org/10.1007/s10863-015-9621-0
---------- VANCOUVER ----------
Chimeo, C., Fernandez-Gimenez, A.V., Campanella, M., Mendez-Romero, O., Muhlia-Almazan, A. The shrimp mitochondrial FoF1-ATPase inhibitory factor 1 (IF1). J. Bioenerg. Biomembr. 2015;47(5):383-393.
http://dx.doi.org/10.1007/s10863-015-9621-0