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Mossuz, P.; Prandini, M.H.; Leroux, D.; Valiron, O.; Schweitzer, A.; Sassetti, B.; Kolodie, L. "Effects of retinoic acid on a new human erythro-megakaryocytic cell line AP-217" (1997) Leukemia Research. 21(6):529-537
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Abstract:

We have characterized a new human cell line AP-217, derived from the peripheral blood of a patient with chronic myeloid leukemia in blastic crisis. The analysis of cell surface antigens and ploidy showed that AP-217 was an erythro-megakaryocytic cell line. The effects of inducers of differentiation were studied and focused on retinoic acid (RA). Uninduced AP-217 cells produced a low level of hemoglobin (Hb) that showed a moderate but significant dose-dependent increase after 13 cis-RA induction (four times above the control at 10-5 M). To outline this effect, AP-217 cells were cloned at limiting dilution. A subclone (clone 2) was isolated which expressed glycophorin A on 12% of cells, and showed a marked sensitivity to RA. After a 4 day induction with increasing concentrations of RA (1-10 x 10-6) Hb production by clone 2 cells was enhanced 12 times over the control at the highest concentration (10-5 M). No effect of RA on the Hb production of K-562 and HEL was observed. This increased Hb production occurred simultaneously with a growth inhibition in clonogenic cultures (20% reduction) associated with a drastic reduction of the colony size. Moreover, we demonstrated the expression of mRNA for the β globin gene in clone 2 and AP-217-cells. This is the first report of a positive effect of RA on the erythroid differentiation of a human leukemic cell line.

Registro:

Documento: Artículo
Título:Effects of retinoic acid on a new human erythro-megakaryocytic cell line AP-217
Autor:Mossuz, P.; Prandini, M.H.; Leroux, D.; Valiron, O.; Schweitzer, A.; Sassetti, B.; Kolodie, L.
Filiación:Laboratoire d'Hématologie, CHU A. Michallon, 38043 Grenoble, Cedex 9, France
CEA INSERM U217 DBMS CEN, Grenoble, France
Departamento de Quimica Biologica, Fac. de Cie. Exactas y Naturales, Universidad de Buenos Aires, Buenos Aires, Argentina
Lab. de Genet.-Oncohematologique, Faculté de Médecine, Grenoble, France
Palabras clave:Erythromegakaryocytic cell lines; Hb production; Leukemic cell differentiation; Retinoic acid; beta globin; globin messenger rna; glycophorin a; hemoglobin; membrane antigen; retinoic acid; adult; article; blast cell crisis; cell differentiation; cell growth; cell line; cell strain k 562; chronic myeloid leukemia; clonogenesis; controlled study; dose response; drug sensitivity; erythroid cell; globin gene; growth inhibition; hemoglobin synthesis; human; human cell; leukemia cell; male; megakaryocyte; ploidy; priority journal; Adult; Antigens, Surface; Blotting, Northern; Cell Differentiation; Cell Division; Cloning, Molecular; Erythrocytes; Globins; Hemoglobins; Humans; Isotretinoin; Karyotyping; Leukemia, Myeloid, Chronic; Male; Megakaryocytes; Polymerase Chain Reaction; Tumor Cells, Cultured
Año:1997
Volumen:21
Número:6
Página de inicio:529
Página de fin:537
DOI: http://dx.doi.org/10.1016/S0145-2126(97)00008-8
Título revista:Leukemia Research
Título revista abreviado:LEUK. RES.
ISSN:01452126
CODEN:LERED
CAS:Antigens, Surface; Globins, 9004-22-2; Hemoglobins; Isotretinoin, 4759-48-2
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01452126_v21_n6_p529_Mossuz

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Citas:

---------- APA ----------
Mossuz, P., Prandini, M.H., Leroux, D., Valiron, O., Schweitzer, A., Sassetti, B. & Kolodie, L. (1997) . Effects of retinoic acid on a new human erythro-megakaryocytic cell line AP-217. Leukemia Research, 21(6), 529-537.
http://dx.doi.org/10.1016/S0145-2126(97)00008-8
---------- CHICAGO ----------
Mossuz, P., Prandini, M.H., Leroux, D., Valiron, O., Schweitzer, A., Sassetti, B., et al. "Effects of retinoic acid on a new human erythro-megakaryocytic cell line AP-217" . Leukemia Research 21, no. 6 (1997) : 529-537.
http://dx.doi.org/10.1016/S0145-2126(97)00008-8
---------- MLA ----------
Mossuz, P., Prandini, M.H., Leroux, D., Valiron, O., Schweitzer, A., Sassetti, B., et al. "Effects of retinoic acid on a new human erythro-megakaryocytic cell line AP-217" . Leukemia Research, vol. 21, no. 6, 1997, pp. 529-537.
http://dx.doi.org/10.1016/S0145-2126(97)00008-8
---------- VANCOUVER ----------
Mossuz, P., Prandini, M.H., Leroux, D., Valiron, O., Schweitzer, A., Sassetti, B., et al. Effects of retinoic acid on a new human erythro-megakaryocytic cell line AP-217. LEUK. RES. 1997;21(6):529-537.
http://dx.doi.org/10.1016/S0145-2126(97)00008-8