Abstract:
The effect of β-cyclodextrin modifications (polymerization (PCD) and later carboxymethylation (CMPCD)) on their action as enzyme stabilizers was analyzed during freeze-drying and thermal treatment. Combined polymer-trehalose matrices were also employed. Due to their higher Tg values, PCD and CMPCD provided better structural stability to the freeze-dried formulations than β-CD. However, only PCD was a good excipient to protect invertase both in amorphous and supercooled systems. FT-IR revealed increased protein denaturation in the presence of CMPCD, but not in the presence of PCD. Even though all polymers inhibited/delayed trehalose crystallization, only trehalose (T) combined with PCD (PCD + T) and with β-cyclodextrin (β-CD + T) offered the best stability to the enzyme. In β-CD + T system, trehalose was the main responsible for the protection. In PCD + T system, both additives contributed to improve the enzyme stability. FT-IR and DSC were useful to analyze the combined role of molecular and supramolecular interactions on enzyme stability in dehydrated model systems. © 2010 Elsevier Ltd.
Registro:
Documento: |
Artículo
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Título: | β-cyclodextrin modifications as related to enzyme stability in dehydrated systems: Supramolecular transitions and molecular interactions |
Autor: | Santagapita, P.R.; Brizuela, L.G.; Mazzobre, M.F.; Ramírez, H.L.; Corti, H.R.; Santana, R.V.; Buera, M.P. |
Filiación: | Departamento de Industrias y Departamento de Química Orgánica, Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria, 1428 Buenos Aires, Argentina Centro de Estudios de Tecnología Enzimática, Facultad de Agronomía, Universidad de Matanzas Camilo Cienfuegos, Autopista a Varadero km 3, 44740 Matanzas, Cuba Departamento de Física de la Materia Condensada, Comisión Nacional de Energía Atómica, Centro Atómico Constituyentes, Avda. Constituyentes, San Martín, Buenos Aires, Argentina Instituto de Química Física de Los Materiales, Ambiente y Energía (INQUIMAE), Facultad de Ciencias Exactas y Naturales, Ciudad Universitaria, 1428 Buenos Aires, Argentina Department of Analytical Chemistry, Faculty of Chemistry, Complutense University of Madrid, Madrid, Spain
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Palabras clave: | Additives; Enzyme stability; Freeze-dried enzyme formulations; Modified cyclodextrins; Polycyclodextrin; Carboxymethylation; Enzyme stability; Freeze-dried enzymes; Freeze-dried formulations; Higher T; Model system; Modified cyclodextrins; Polycyclodextrin; Protein denaturation; Structural stabilities; Supramolecular interactions; T-Systems; Thermal treatment; Cyclodextrins; Data storage equipment; Enzymes; Polymers; Silanes; Supramolecular chemistry; System stability |
Año: | 2011
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Volumen: | 83
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Número: | 1
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Página de inicio: | 203
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Página de fin: | 209
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DOI: |
http://dx.doi.org/10.1016/j.carbpol.2010.07.041 |
Título revista: | Carbohydrate Polymers
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Título revista abreviado: | Carbohydr Polym
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ISSN: | 01448617
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CODEN: | CAPOD
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Registro: | https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01448617_v83_n1_p203_Santagapita |
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Citas:
---------- APA ----------
Santagapita, P.R., Brizuela, L.G., Mazzobre, M.F., Ramírez, H.L., Corti, H.R., Santana, R.V. & Buera, M.P.
(2011)
. β-cyclodextrin modifications as related to enzyme stability in dehydrated systems: Supramolecular transitions and molecular interactions. Carbohydrate Polymers, 83(1), 203-209.
http://dx.doi.org/10.1016/j.carbpol.2010.07.041---------- CHICAGO ----------
Santagapita, P.R., Brizuela, L.G., Mazzobre, M.F., Ramírez, H.L., Corti, H.R., Santana, R.V., et al.
"β-cyclodextrin modifications as related to enzyme stability in dehydrated systems: Supramolecular transitions and molecular interactions"
. Carbohydrate Polymers 83, no. 1
(2011) : 203-209.
http://dx.doi.org/10.1016/j.carbpol.2010.07.041---------- MLA ----------
Santagapita, P.R., Brizuela, L.G., Mazzobre, M.F., Ramírez, H.L., Corti, H.R., Santana, R.V., et al.
"β-cyclodextrin modifications as related to enzyme stability in dehydrated systems: Supramolecular transitions and molecular interactions"
. Carbohydrate Polymers, vol. 83, no. 1, 2011, pp. 203-209.
http://dx.doi.org/10.1016/j.carbpol.2010.07.041---------- VANCOUVER ----------
Santagapita, P.R., Brizuela, L.G., Mazzobre, M.F., Ramírez, H.L., Corti, H.R., Santana, R.V., et al. β-cyclodextrin modifications as related to enzyme stability in dehydrated systems: Supramolecular transitions and molecular interactions. Carbohydr Polym. 2011;83(1):203-209.
http://dx.doi.org/10.1016/j.carbpol.2010.07.041