Artículo

Castro, P.M.; Sousa, F.; Magalhães, R.; Ruiz-Henestrosa, V.M.P.; Pilosof, A.M.R.; Madureira, A.R.; Sarmento, B.; Pintado, M.E. "Incorporation of beads into oral films for buccal and oral delivery of bioactive molecules" (2018) Carbohydrate Polymers. 194:411-421
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Abstract:

The association of alginate beads and guar-gum films in a single delivery system was idealized to promote a more effective buccal and oral delivery of bioactive molecules. A response surface method (experimental design approach) was performed to obtain optimal formulations of alginate beads to be incorporated into guar gum oral films as combined buccal and oral delivery systems for caffeine delivery. The combined formulation was further characterized regarding physicochemical properties, drug release, cell viability and buccal permeability. Beads average size, determined by dynamic light scattering (DLS), was of 3.37 ± 6.36 μm. Film thickness was set to 62 μm. Scanning electron microscopy micrographs revealed that beads were evenly distributed onto the film matrix and beads size was in accordance to data obtained from DLS analysis. Evaluation of Fourier-transform infrared spectra did not indicate the formation of new covalent bonds between the matrix of guar-gum films, alginate beads and caffeine. In vitro release assays by dialysis membrane allowed understanding that the combination of guar-gum films and alginate beads assure a slower release of caffeine when compared with the delivery profile of free caffeine from alginate beads or guar-gum films alone. MTT assay, performed on human buccal carcinoma TR146 cell line, allowed concluding that neither guar-gum film, alginate beads nor guar-gum film incorporated into alginate beads significantly compromised cell viability after 12 h of exposure. As demonstrated by in vitro permeability assay using TR146 human buccal carcinoma cell lines, combination of guar-gum films and alginate beads also promoted a slower release and, thus, lower apparent permeability (1.15E–05 ± 3.50E-06) than for caffeine solution (2.68E–05 ± 7.30E-06), guar-gum film (3.12E–05 ± 4.70E-06) or alginate beads (2.01E–05 ± 3.90E-06). The conjugation of alginate beads within an orodispersible film matrix represents an effective oral/buccal delivery system that induces a controlled release along with an enhanced intimate contact with cell layers that may promote higher in vivo bioavailability of carried drugs. © 2018

Registro:

Documento: Artículo
Título:Incorporation of beads into oral films for buccal and oral delivery of bioactive molecules
Autor:Castro, P.M.; Sousa, F.; Magalhães, R.; Ruiz-Henestrosa, V.M.P.; Pilosof, A.M.R.; Madureira, A.R.; Sarmento, B.; Pintado, M.E.
Filiación:CBQF – Centro de Biotecnologia e Química Fina – Laboratório Associado, Escola Superior de Biotecnologia, Universidade Católica Portuguesa/Porto, Rua Arquiteto Lobão Vital, 172, Porto, 4200-374, Portugal
CESPU, Instituto de Investigação e Formação Avançada em Ciências e Tecnologias da Saúde, Rua Central de Gandra 1317, Gandra-PRD, 4585-116, Portugal
ICBAS – Instituto Ciências Biomédicas Abel Salazar, Universidade do Porto, Porto, 4150-180, Portugal
i3S – Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Rua Alfredo Allen 208, Porto, 4200-393, Portugal
INEB –Instituto Nacional de Engenharia Biomédica, Universidade do Porto, Rua Alfredo Allen 208, Porto, 4200-393, Portugal
Departamento de Industrias, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria, Buenos Aires, 1428, Argentina
Consejo Nacional de Investigaciones Científicas y Tecnicas (CONICET), Argentina
Palabras clave:Alginate beads; Caffeine; Drug delivery; Experimental design; Oral films; Slow release; Alginate; Assays; Biochemistry; Caffeine; Cell culture; Cells; Design of experiments; Dialysis membranes; Drug delivery; Dynamic light scattering; Molecules; Scanning electron microscopy; Statistics; Targeted drug delivery; Alginate beads; Apparent permeabilities; Carcinoma cell lines; Experimental design approaches; Fourier transform infrared spectra; Physicochemical property; Response surface method; Slow release; Controlled drug delivery; alginic acid; caffeine; drug carrier; galactan; glucuronic acid; guar gum; hexuronic acid; mannan; plant gum; cell survival; chemistry; drug delivery system; drug effect; human; oral drug administration; tumor cell line; Administration, Oral; Alginates; Caffeine; Cell Line, Tumor; Cell Survival; Drug Carriers; Drug Delivery Systems; Galactans; Glucuronic Acid; Hexuronic Acids; Humans; Mannans; Plant Gums
Año:2018
Volumen:194
Página de inicio:411
Página de fin:421
DOI: http://dx.doi.org/10.1016/j.carbpol.2018.04.032
Título revista:Carbohydrate Polymers
Título revista abreviado:Carbohydr Polym
ISSN:01448617
CODEN:CAPOD
CAS:alginic acid, 28961-37-7, 29894-36-8, 9005-32-7, 9005-38-3; caffeine, 58-08-2; galactan, 39300-87-3, 9037-55-2; glucuronic acid, 36116-79-7, 576-37-4, 6556-12-3; guar gum, 9000-30-0; mannan, 51395-96-1, 9036-88-8; Alginates; alginic acid; Caffeine; Drug Carriers; Galactans; Glucuronic Acid; guar gum; Hexuronic Acids; Mannans; Plant Gums
Registro:https://bibliotecadigital.exactas.uba.ar/collection/paper/document/paper_01448617_v194_n_p411_Castro

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Citas:

---------- APA ----------
Castro, P.M., Sousa, F., Magalhães, R., Ruiz-Henestrosa, V.M.P., Pilosof, A.M.R., Madureira, A.R., Sarmento, B.,..., Pintado, M.E. (2018) . Incorporation of beads into oral films for buccal and oral delivery of bioactive molecules. Carbohydrate Polymers, 194, 411-421.
http://dx.doi.org/10.1016/j.carbpol.2018.04.032
---------- CHICAGO ----------
Castro, P.M., Sousa, F., Magalhães, R., Ruiz-Henestrosa, V.M.P., Pilosof, A.M.R., Madureira, A.R., et al. "Incorporation of beads into oral films for buccal and oral delivery of bioactive molecules" . Carbohydrate Polymers 194 (2018) : 411-421.
http://dx.doi.org/10.1016/j.carbpol.2018.04.032
---------- MLA ----------
Castro, P.M., Sousa, F., Magalhães, R., Ruiz-Henestrosa, V.M.P., Pilosof, A.M.R., Madureira, A.R., et al. "Incorporation of beads into oral films for buccal and oral delivery of bioactive molecules" . Carbohydrate Polymers, vol. 194, 2018, pp. 411-421.
http://dx.doi.org/10.1016/j.carbpol.2018.04.032
---------- VANCOUVER ----------
Castro, P.M., Sousa, F., Magalhães, R., Ruiz-Henestrosa, V.M.P., Pilosof, A.M.R., Madureira, A.R., et al. Incorporation of beads into oral films for buccal and oral delivery of bioactive molecules. Carbohydr Polym. 2018;194:411-421.
http://dx.doi.org/10.1016/j.carbpol.2018.04.032